Development of highly sensitive biosensors based on Hetero-core fiber optics

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K14173
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 21030:Measurement engineering-related
• Research Institution: University of Toyama (2022-2023); National Institute of Genetics (2021)
• Principal Investigator: Hosoki Ai 富山大学, 学術研究部理学系, 特命助教 (30748835)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: ヘテロコア光ファイバ / バイオセンサ / 局在表面プラズモン共鳴 / DNA

Outline of Final Research Achievements

The aim of this study is to construct an innovative biosensor based on Hetero-core fiber optics as a new technological platform for direct and highly sensitive detection of extremely small amounts of target nucleic acid. Methods for immobilizing probe nucleic acid were investigated using peptide bonds and biotin-avidin complexes, and Hetero-core optical fiber biosensors using surface plasmon resonance (SPR) and localized surface plasmon resonance (LSPR) were developed. We investigated in detail the detection sensitivity depending on the concentration of the target nucleic acid, and clarified the concentration which can be detected as extremely small by the sensors.

Free Research Field

光ファイバセンサ、ケミカルセンシング

Academic Significance and Societal Importance of the Research Achievements

コロナウイルス感染症に伴い、PCR検査装置の需要が一気に高まった。近年では、迅速・高感度に特定の核酸を検出可能なセンサデバイスの実現が期待されている。本研究では、ヘテロコア光ファイバセンサの高感度化を図り、標的核酸を高感度に検出可能なバイオセンサの開発に取り組んだ。センサ感度やセンサ作製の再現性には課題が残ったものの、本研究によって、SPRやLSPRによるヘテロコア光ファイババイオセンサの基盤技術を確立できた。