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2021 Fiscal Year Research-status Report

Exploring the functional role of Hijiki in obesity-associated to sphingolipid metabolism

Research Project

Project/Area Number 21K14812
Research InstitutionHokkaido University

Principal Investigator

BommeGowda SiddabasaveGowda  北海道大学, 保健科学研究院, 講師 (50800504)

Project Period (FY) 2021-04-01 – 2025-03-31
KeywordsHijiki / obesity / Sphingolipid metabolism
Outline of Annual Research Achievements

The main goal of the project is to find the active constituent of hijiki responsible for sphingomyelin synthase inhibition. So far, we have completed the methanol extraction of hijiki plant material in bulk and separated the active constituents based on solvent partition. All the solvent partitions are subjected to SMS inhibition screening assay using high-performance liquid chromatography with fluorescence detection. As consistent with our preliminary results, we found the strong SMS inhibition in hexane fraction, and it's further fractionated into several fractions. The SMS screening assay results further confirmed the active compound in fraction 3. The high-resolution mass spectrometry and NMR analysis was performed to reveal the structural information of the active compound.

Current Status of Research Progress
Current Status of Research Progress

2: Research has progressed on the whole more than it was originally planned.

Reason

Based on the analysis results, we judged that molecular weight of the compound is between 300-330,suggesting it as a small molecule inhibitor. Further, the active compound is a mixture of lipids belong to fatty acid family. As we found that the active constituent of hijiki is an inseparable isomeric mixture, there is delay in invitro experiments. We need to confirm the structure and purify the active constituent in order to reveal the in vitro actions of the hijiki derived SMS inhibitor. As a first effort, we made derivatization with a UV chromophore to separate the isomers using HPLC system. We are making all our efforts on the purification process. As soon as we accomplished it, the rest of the aims will be focused.

Strategy for Future Research Activity

In the future, we will focus on the purification of active constituent using HPLC in order to confirm the structure of the major isomers. HRMS and NMR analysis will be formed. Upon obtaining the active constituent of hijiki in mg scale by HPLC purification, the SMS screening assay will be performed to know the inhibitory concentrations. Then the action of active constituent on lipid droplet accumulation in fatty liver cell model will be studied as described in the proposal aim 2.

Causes of Carryover

Not applicable

  • Research Products

    (3 results)

All 2022 2021

All Journal Article (2 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (1 results) (of which Invited: 1 results)

  • [Journal Article] Simple and Sensitive Method for the Quantitative Determination of Lipid Hydroperoxides by Liquid Chromatography/Mass Spectrometry2022

    • Author(s)
      Liang Chongsheng、B. Gowda Siddabasave Gowda、Gowda Divyavani、Sakurai Toshihiro、Sazaki Iku、Chiba Hitoshi、Hui Shu-Ping
    • Journal Title

      Antioxidants

      Volume: 11 Pages: 229~229

    • DOI

      10.3390/antiox11020229

    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Defining the kinetic effects of infection with influenza virus A/PR8/34 (H1N1) on sphingosine-1-phosphate signaling in mice by targeted LC/MS2021

    • Author(s)
      Gowda Divyavani、Ohno Marumi、B. Gowda Siddabasave Gowda、Chiba Hitoshi、Shingai Masashi、Kida Hiroshi、Hui Shu-Ping
    • Journal Title

      Scientific Reports

      Volume: 11 Pages: 20161

    • DOI

      10.1038/s41598-021-99765-0

    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Global lipid fingerprinting for early disease biomarker discovery2021

    • Author(s)
      Siddabasave Gowda B. Gowda
    • Organizer
      The 46th Annual meeting of the Japanese Society for Biomedical Mass Spectrometry
    • Invited

URL: 

Published: 2022-12-28  

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