Localization analysis of SIX3 effector in Fusarium oxysporum f. sp. cepae

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K14857
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 39040:Plant protection science-related
• Research Institution: Yamaguchi University
• Principal Investigator: Sasaki Kazunori 山口大学, 大学研究推進機構, 准教授 (50766318)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: タマネギ乾腐病 / エフェクター / シャロット / フザリウム

Outline of Final Research Achievements

The aim of this study was to establish the functions and host localizations of FocSIX3 and FocSIX5 effector in Fusarium oxysporum f. sp. cepae. FocSIX3 and FocSIX5 were localized to the apoplastic region in basal plate of susceptible onion plants, but not that in resistant shallots, suggesting that their co-localization in apoplastic region of basal plate is involved in their pathogenicity. In addition, this study revealed that FocSIX5 acts as an avirulence gene against shallots. FocSIX5-receptor candidate genes in shallot were selected, which is expected to be applied to resistance breeding for onion basal rot disease.

Free Research Field

植物病理学

Academic Significance and Societal Importance of the Research Achievements

タマネギ乾腐病は、世界中で発生しているタマネギの重要病害であるが、その感染メカニズムは未解明の部分が多く、完全な耐病性品種も存在しない。本研究により得られた知見は、タマネギ乾腐病菌の感染メカニズム解明の一端を担うものであるとともに、非病原力遺伝子を標的としたタマネギ乾腐病耐病性育種への応用が期待できる。