2023 Fiscal Year Final Research Report
Anti-Obesity Effect of Spice Constituents: The Functional Diversity of Vanilloid with Different Points of Action
| Project/Area Number |
21K14889
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 40020:Wood science-related
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| Research Institution | Tohoku University (2023)
Nagoya University (2021-2022) |
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Principal Investigator |
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| Project Period (FY) |
2021-04-01 – 2024-03-31
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| Keywords | Spice / Grains of Paradise / Vanilloid / Anti-obesity / Photoaffinity labeling / 6-paradol / 6-gingerol / Proteomics |
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| Outline of Final Research Achievements |
The purpose of this study is to elucidate the molecular mechanism of the anti-obesity effect demonstrated by Grains of Paradise (GOP), a spice native to West Africa. GOP vanilloids, despite having very similar structures, exhibited different effects on fat accumulation and lipid metabolism. Therefore, photoaffinity probes for several GOP vanilloids were synthesized. A probe of 6-paradol was administered to mouse 3T3-L1 fibroblasts (pre-adipocytes), and specific protein bands that interacted with 6-paradol were detected. Proteomics analysis identified several candidates for target proteins of 6-paradol, suggesting involvement in energy metabolism and nuclear transcription.
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| Free Research Field |
食品化学
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| Academic Significance and Societal Importance of the Research Achievements |
肥満抑制は特に先進国において重要な解決課題の一つであり，これまでに様々な抗肥満成分の探索が行われてきた。しかしながら，化学構造がわずかに違う化合物が各組織でどのタンパク質を標的とするかを調査した例はほとんどなかった。また，トウガラシの代表的な辛味バニロイドであるカプサイシンの肥満抑制作用は，専ら受容体を介した交感神経系の活性化による脂肪分解であるとみなされている。本研究で扱うバニロイドは，カプサイシンと類似した化学構造を有するが作用が異なる。本研究により，バニロイドの直接相互作用する新たな辛味受容タンパク質の候補が発見できたため，異なる作用点で機能する将来の肥満改善薬開発への貢献が期待できる。
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