2022 Fiscal Year Final Research Report
Structural study of cohesin and HORMAD1 in meiotic chromosome axis formation
| Project/Area Number |
21K15032
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 43020:Structural biochemistry-related
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| Research Institution | Nara Medical University |
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Principal Investigator |
Kikuchi Sotaro 奈良県立医科大学, 医学部, 助教 (90866386)
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| Project Period (FY) |
2021-04-01 – 2023-03-31
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| Keywords | 構造生物化学 |
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| Outline of Final Research Achievements |
The aim of this study was to clarify the steric structure of Human HORMAD1 using X-ray crystallography and to analyze its interaction with cohesin biochemically and cell biologically to obtain the structural basis of HORMAD1 in meiosis. We succeeded in preparing highly purified human HORMAD1 using an E. coli expression system and obtained sufficient yield for X-ray analysis, but did not obtain crystals for structural analysis. It was suggested that HORMAD1 had multiple conformations during the purification process, which may have affected crystallization.
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| Free Research Field |
構造生物化学
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| Academic Significance and Societal Importance of the Research Achievements |
私たちの遺伝情報を担うDNA は、細胞分裂時に染色体として高度に折り畳まれ娘細胞へと均等に分配されていく。染色体の均等分配に関わるタンパク質複合体コヒーシンは 複製や修復など様々な機能に関与しており、その異常は遺伝性疾患や不妊、がんなどを引き起こすことが知られている。本研究対象であるHORMAD1は減数分裂型の制御に関わっており、本研究から示唆されたHORMADの複数のコンフォーメーション変化が、減数分裂特有の制御メカニズムに深く関わっていることが考えられた。
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