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2023 Fiscal Year Final Research Report

Mechanism of autophagosome-selective membrane degradation by Atg15

Research Project

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Project/Area Number 21K15045
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 43030:Functional biochemistry-related
Research InstitutionMicrobial Chemistry Research Foundation

Principal Investigator

Maruyama Tatsuro  公益財団法人微生物化学研究会, 微生物化学研究所, 研究員 (60795855)

Project Period (FY) 2021-04-01 – 2024-03-31
Keywordsオートファジー / リパーゼ
Outline of Final Research Achievements

Autophagy is an intracellular degradation system, and the degradation of autophagosomes, which are double membrane vesicles, in the vacuole is important. The inner membrane vesicles of autophagosomes released by fusion with the vacuole are thought to be degraded by Atg15, an intravacuolar lipase. However, it is not clear how Atg15 selectively acts on the autophagosome inner membrane without acting on the vacuolar membrane. In this study, we examined the expression and purification of Atg15 with reference to highly accurate structure prediction and discussed the mechanism of membrane lipid degradation.

Free Research Field

構造生物学

Academic Significance and Societal Importance of the Research Achievements

液胞(リソソーム)における選択的な脂質膜分解は、ヒトを含む真核生物に普遍的な現象であるが、そのメカニズムの詳細は解明されていない。Atg15は、520アミノ酸残基から成るリパーゼと推定されているが、リパーゼ活性を担う通常の構造ドメインよりも約200アミノ酸残基以上も長いことから、Atg15に固有な機能に関連した構造領域を有することが強く示唆される。高精度立体構造予測が得られたことで、Atg15の酵素活性の制御の仕組みを理解する足掛かりになることが期待される。

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Published: 2025-01-30  

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