2022 Fiscal Year Final Research Report
Study of turn over mechanisms of tight junction
| Project/Area Number |
21K15089
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 44010:Cell biology-related
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Project Period (FY) |
2021-04-01 – 2023-03-31
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| Keywords | tight junction / cholesterol / ZO / claudin |
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| Outline of Final Research Achievements |
It has been widely accepted that the formation of tight junctions (TJ) requires ZO proteins, a scaffold protein. However, I found that proteasome inhibitors can induce TJ formation even in cell loss of ZO proteins. This result indicated that factors other than ZO proteins exist in TJ formation. Further analysis revealed a mechanism of TJ formation in which ZO proteins accumulate cholesterol and claudin accumulates in the cholesterol-rich membrane regions.
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| Free Research Field |
細胞生物学
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| Academic Significance and Societal Importance of the Research Achievements |
タイトジャンクションの形成には、他の細胞接着構造での解析を基にした類推から、裏打ちタンパク質であるZOタンパク質とclaudinの結合が必須であると考えられてきた。しかし、本研究によって、ZOタンパク質との結合は必須ではなく、ZOタンパク質がコレステロールを集積させ、コレステロールに富んだ膜領域にclaudinが集積し、タイトジャンクションの形成に至るという、他の細胞接着構造とは異なる形成機構を有していることを明らかにした点は学術的に大きな意義があると考える。
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