The Role of AhR in carcinogenesis from inflammatory bowel disease and its usefulness as a novel tumor marker.

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K15400
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 49020:Human pathology-related
• Research Institution: Shinshu University
• Principal Investigator: Matoba Hisanori 信州大学, 医学部, 特任助教 (10849277)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: AhR / CAD / CAC / p53 / Ki-67 / SATB2 / DNAメチル化

Outline of Final Research Achievements

Immunostaining of AhR for colitis-associated dysplasia (CAD) and colitis-associated carcinoma (CAC) showed positivity in 16 of 19 CAD cases and 4 of 4 CAC cases. SATB2 downregulation was observed in 9 of 19 CAD cases and 4 of 4 CAC cases, and p53 positivity was observed in 5 of 19 CAD cases and 3 of 4 CAC cases. GS-II lectin staining was positive in 15 of 19 CAD cases and 4 of 4 CAC cases. Methylation whole genome sequencing analysis showed no methylation of the AhR promoter region in 8 CAC and CAD cases including AhR-negative cases.

Free Research Field

病理学

Academic Significance and Societal Importance of the Research Achievements

本研究では、AhRがCAD, CACに対する感度の高い診断マーカーとなることが示された。また、GS-IIレクチン染色により染色される糖鎖であるterminal βGlcNAcの増加が、sessile serrated lesion(SSL)およびmicrovesicular hyperplastic polyp(MVHP)とCAD, CACに共通に見られる特徴であることも示された。これらの結果は、CAD, CACに対する正確な診断を可能とし、CAD, CACに対する診療に貢献するものと考えられる。