2022 Fiscal Year Final Research Report
Elucidation of the virulence factor transport mechanism of Entamoeba histolytica based on the retromer complex
Project/Area Number |
21K15426
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Research Category |
Grant-in-Aid for Early-Career Scientists
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Allocation Type | Multi-year Fund |
Review Section |
Basic Section 49040:Parasitology-related
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Research Institution | The University of Tokyo |
Principal Investigator |
Watanabe Natsuki 東京大学, 大学院医学系研究科(医学部), 特任研究員 (00883323)
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Project Period (FY) |
2021-04-01 – 2023-03-31
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Keywords | 赤痢アメーバ / レトロマー / Vps35 |
Outline of Final Research Achievements |
This study, which aims to elucidate the functions of retromer constituents that have diversified in Entamoeba histolytica, has clarified several constituent-factor-specific functions. Notably, Vps35-1 regulates the recruitment of retromers to phagocytic vesicles, and Vps35-2 regulates the activity of secreted cysteine proteases, which are virulence factors. is. In addition, each component forms a different retromeric core complex, and the different core complexes form dimers to tetramers. This study revealed the organization of a highly complex complex of retromers and their distinct functions, especially Vps35-1 and Vps35-2.
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Free Research Field |
分子寄生虫学
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Academic Significance and Societal Importance of the Research Achievements |
レトロマーはパーキンソン病やアルツハイマー病の原因遺伝子であるVps35を含む複合体だが、レトロマー構成因子のisotypeの違いによる役割の違いは未だよく明らかになっていない。本研究により、レトロマー構成因子のそれぞれのisotypeによる異なる働きの一端が明らかとなったことはレトロマーの機能を明らかにするために重要である。 これを軸として、他のisotypeの機能や複合体の形成様式が明らかとなれば、赤痢アメーバのみならず、哺乳類における上記の疾病の原因解明と新規治療方法の発見に繋がことが期待される。
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