2022 Fiscal Year Final Research Report
Development of a neuronal network evaluation system and application to brain disease research using wide-field two-photon microscopy.
| Project/Area Number |
21K15635
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 51030:Pathophysiologic neuroscience-related
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| Research Institution | Tohoku University (2022)
National Institutes for Quantum Science and Technology (2021) |
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Principal Investigator |
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| Project Period (FY) |
2021-04-01 – 2023-03-31
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| Keywords | in vivoイメージング / 神経ネットワーク / 二光子顕微鏡 |
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| Outline of Final Research Achievements |
The purpose of the present study was to elucidate learning-induced changes in neuronal network on a single cellular level. Calcium imaging using a wide-field two-photon laser microscope was performed on mice conditioned with fear memories to record the activity of thousands of neurons in the cerebral cortex. A novel evaluate method developed to detect changes in neuronal networks revealed changes in brain networks associated with memory formation.
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| Free Research Field |
神経科学
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| Academic Significance and Societal Importance of the Research Achievements |
これまでの脳神経機能研究では単一細胞レベルの空間分解能で神経ネットワークの機能及びその変化を評価する方法は実現されていなかったため、脳疾患や加齢に伴う神経ネットワークの変化についての調査は不十分であった。本研究で開発された評価手法は脳疾患研究における脳機能障害の理解や治療法開発だけでなく、基礎神経科学における神経回路の解読や制御などにも役立つことが考えられる。
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