Development of non-invasive biomarker to detect colorectal cancer using urinary long non-coding RNA

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K15956
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 53010:Gastroenterology-related
• Research Institution: Nagoya City University
• Principal Investigator: Iwasaki Hiroyasu 名古屋市立大学, 医薬学総合研究院(医学), 研究員 (30867627)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: バイオマーカー / 大腸癌 / 尿

Outline of Final Research Achievements

In order to establish diagnostic biomarkers using urinary long noncoding RNA (lncRNA) for colorectal cancer (CRC), we performed comprehensive analysis with RNA sequence, which identified several lncRNAs with aberrant expression between normal tissues and CRC tissues. Since urinary expression levels of lncRNAs were relatively low, only 3 urinary lncRNAs were finally selected as biomarker candidates for early diagnosis of CRC. However, the significance of these biomarkers were limited in a large sample cohort.
On the other hand, another urinary biomarker instead of urinary lncRNA was identified as a potential biomarker in the present study, and we confirmed the significance in another independent cohort.

Free Research Field

消化器内科

Academic Significance and Societal Importance of the Research Achievements

尿中long noncoding RNA(lncRNA)をパネル化することによる大腸癌診断バイオマーカーの樹立を目指し、本研究をすすめた。大腸癌患者の尿中で異常発現をきたす複数のlncRNAは同定したものの、残念ながら実用化にいたるような高い水準の尿中lncRNAバイオマーカーを樹立するに至らなかった。しかしながら、本研究の副産物として、大腸癌診断を可能とする別の尿中バイオマーカー因子を見出したため、今後の実用化が期待される。