2022 Fiscal Year Final Research Report
Function of neuropeptide, TIP39, in bone marrow microenvironment with hematopoietic neoplasm
Project/Area Number |
21K16273
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Research Category |
Grant-in-Aid for Early-Career Scientists
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Allocation Type | Multi-year Fund |
Review Section |
Basic Section 54010:Hematology and medical oncology-related
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Research Institution | Sapporo Medical University |
Principal Investigator |
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Project Period (FY) |
2021-04-01 – 2023-03-31
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Keywords | neuropeptide / acute myeloid leukemia / TIP39 |
Outline of Final Research Achievements |
First, we analyzed proliferation of AML cell lines under TIP39 stimulation. The difference has not observed between culture with TIP39 and without TIP39. Next, we conducted AML cell culture without serum, in starved condition, to research the effect to survival of AML cell. Surprisingly, TIP39-stimulated AML cells were significantly survival under starved condition. Moreover, increased LC3-II expression in TIP39-stimulated AML cell at one to two hours after the stimulation was observed compared to unstimulated AML cell by western blot analysis. In addition, under normal culture the increased LC3-II was also revealed in the stimulated cells. Furthermore, the proportion of apoptosis in TIP39-stimulated AML cell was decreased compared to unstimulated AML cell. These results suggest that TIP39-induced autophagy could affect survival advantage over normal cell.
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Free Research Field |
Hematology
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Academic Significance and Societal Importance of the Research Achievements |
オートファジーは正常細胞でも細胞生存のため必要な機構であるが、悪性腫瘍でも細胞生存とアポトーシスに大きく関与しており、治療標的としても注目され、それを標的とした薬剤が腎臓がんや乳がんに治療薬として用いられている。神経ペプチドであるTIP39がオートファジーを誘導することは、いままで報告はなく、白血病細胞株での効果も明らかではなかった。 今回TIP39がオートファジーを誘導することが明らかになり、白血病細胞株で細胞生存に関わることが明らかとなることで、造血器腫瘍のみならず固形癌への波及効果が期待される。
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