Analysis of anti-tumor drug resistance of esophageal cancer derived cancer stem-like cells

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K16448
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 55020:Digestive surgery-related
• Research Institution: Yamaguchi University
• Principal Investigator: Watanabe Yusaku 山口大学, 医学部附属病院, 助教 (80799437)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: 癌

Outline of Final Research Achievements

In this study, cancer stem-like cells (CSLC) were induced from eight human esophageal cancer cell lines, including KYSE30 and KYSE70. non-sphere-forming KYSE30 cells did not become resistant to anti-cancer drugs under sphere induction medium (SIM) culture, whereas the shpere-forming KYSE70 and YES2 cells became resistant to anti-cancer drugs, such as 5-FU, irinotecan, and cisplatin. Furthermore, an interesting correlation was observed between RAB gene expression/exosomes/Sphere-forming ability/anti-cancer drug resistance.

Free Research Field

消化器外科

Academic Significance and Societal Importance of the Research Achievements

本研究によって、ヒト食道癌細胞株より抗がん剤耐性の亢進するcancer stem-like sphere cells (CSLCs) を誘導することができた。この食道癌CSLCの誘導にはRAB4Bやエクソソームが関与していることが示唆され、食道癌に対する新たな治療標的につながる成果が得られた。