Elucidation of the pathogenesis of paroxysmal sympathetic nerve hyperactivity after subarachnoid hemorrhage focusing on IL-17 signaling and development of new treatment methods

2022 Fiscal Year Final Research Report

• Project/Area Number: 21K16589
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 55060:Emergency medicine-related
• Research Institution: Shimane University
• Principal Investigator: Miyajima Hisao 島根大学, 学術研究院医学・看護学系, 助教 (80874362)
• Project Period (FY): 2021-04-01 – 2023-03-31
• Keywords: 脳血管障害 / くも膜下出血 / サイトカイン / 交感神経

Outline of Final Research Achievements

Paroxysmal sympathetic hyperactivity (PSH) is known as one of the complications immediately after subarachnoid hemorrhage (SAH), but the detailed pathomechanism is still unclear. In this study, we focused on the inflammatory cytokine IL-17 to identify IL-17-secreting cells in SAH and to elucidate the pathogenesis of PSH. We investigated the expression levels of various cytokines in the brain parenchyma, meninges, blood, and cerebrospinal fluid by ELISA and quantitative RT-PCR. Then, using transgenic mice, we examined IL-17-expressing cells in the brain parenchyma and meninges by histological analysis using immunostaining methods. In addition, we developed a novel IL-17 antibody to validate the therapeutic effects of PSH.

Free Research Field

神経免疫学

Academic Significance and Societal Importance of the Research Achievements

これまでも、PSHはSAH等の脳損傷後の合併症として高頻度に発症することが報告されているが、PSHの病態メカニズムや異常神経回路に関しての詳細は不明である。したがって、今後の研究成果によって、SAH後のPSHにおけるIL-17シグナルの関与とそのメカニズムを解明できれば、SAHのみならず、頭部外傷における脳損傷などのPSH予防・治療法開発にもつながることが期待される。