Estimation of renal function by measuring D-amino acids using a two-dimensional high-performance liquid chromatography system

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K16730
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 56030:Urology-related
• Research Institution: Osaka University
• Principal Investigator: Kawamura Masataka 大阪大学, 大学院医学系研究科, 招へい教員 (00808925)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: D-アミノ酸 / D-セリン / D-セリンクリアランス / 腎機能 / 腎移植

Outline of Final Research Achievements

Our specially developed two-dimensional high-performance liquid chromatography system has made it possible to detect D-amino acids with high sensitivity. In this study, we analyzed the correlation between D-amino acids and measured renal function using inulin clearance, and examined whether D-amino acids could be used to estimate renal function.
D-amino acids were measured in plasma and urine of living donors and recipients of renal transplants.
D-serine clearance was strongly correlated with glomerular filtration rate and showed less bias than conventional markers of renal function. We also developed an equation for predicting renal function using D-serine clearance and found that the combination of the two equations enabled accurate calculation of GFR.

Free Research Field

腎移植

Academic Significance and Societal Importance of the Research Achievements

慢性腎臓病とその悪化による透析療法導入は、人口高齢化に伴ってさらなる増加が懸念され、世界的に社会、医療、経済上の深刻な問題となっている。本研究成果を利用することによって腎機能を正確に評価することで、腎臓病の早期診断や重症度の正確な判定、腎障害時の投薬量の決定、などが可能となり、腎臓病の治療法改善を通じて人工透析導入の抑制なども期待される。また、D-セリンをバイオマーカーとして、新規治療薬の開発にもつながることが期待される。