A novel ovarian cancer mouse model derived from genetically engineered organoid

2022 Fiscal Year Final Research Report

• Project/Area Number: 21K16790
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 56040:Obstetrics and gynecology-related
• Research Institution: Osaka University
• Principal Investigator: Nakamura Koji 大阪大学, 大学院医学系研究科, 助教 (00900151)
• Project Period (FY): 2021-04-01 – 2023-03-31
• Keywords: 卵巣がん / オルガノイド / モデルマウス / 遺伝子改変 / CRISPR/Cas9 / 卵巣癌 / マウスモデル / GEMM

Outline of Final Research Achievements

The goal of this study is to establish an organoid-derived ovarian cancer mouse model in which mouse ovarian cancer is generated in vitro by applying oncogenic stimuli to organoids established from the oviducts of immunocompetent mice and transplanted orthotopically into mice. We first isolated oviducts from immunocompetent mice and established organoid culture. We also confirmed that the organoids are derived from the oviduct. CRISPR plasmids for oncogenic stimulation were successfully cloned. As a future plan, we aim to establish ovarian cancer organoids and further transplant them orthotopically into mice.

Free Research Field

産婦人科、卵巣がん

Academic Significance and Societal Importance of the Research Achievements

本研究の成果は今後、ヒト卵巣がんの病態を再現しつつ、安価でスピーディな、かつ特別な設備がなくとも作製できる卵巣がんマウスモデルを樹立へと繋がるものである。そしてこのマウスモデルが樹立されれば卵巣がんの新規治療法の確立のみならず、進展機構の解明、早期診断法の確立など、卵巣がんの予後改善に向けた研究に広く役立てることが可能となる。本研究のマウスモデルが確立されれば、多くの研究者に応用されることが期待され、世界中の卵巣がん研究を加速させる可能性があると考えている。