Regulation of inflammation in dialysis foot ulcers using amnion-derived mesenchymal stem cells

2022 Fiscal Year Final Research Report

• Project/Area Number: 21K16913
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 56070:Plastic and reconstructive surgery-related
• Research Institution: Hokkaido University
• Principal Investigator: Takahashi Hiroko 北海道大学, 医学研究院, 客員研究員 (00829434)
• Project Period (FY): 2021-04-01 – 2023-03-31
• Keywords: 羊膜由来間葉系幹細胞 / Klotho / 透析 / 創傷 / 再生医療

Outline of Final Research Achievements

The number of patients requiring dialysis in Japan has been increasing significantly, leading to a rise in refractory chronic wounds such as critical limb ischemia and calciphylaxis, which have become a significant social issue. Mesenchymal stem cells have been extensively utilized in treating various illnesses and have demonstrated efficacy in promoting wound healing. In this investigation, we evaluated the impact of local administration of amnion-derived mesenchymal stem cells (AMSCs) on wound healing in Klotho mice, which possess kinetics comparable to patients with end-stage renal failure. To create a back wound model, AMSC hypoxic culture supernatant was solidified and applied topically to the ulcer site, which considerably enhanced the rate of wound contraction in Klotho mice. Ulcer tissue was collected and assessed through real-time PCR, which indicated that AMSC topical application suppressed the expression of MMP-3 and MMP-9 in Klotho mice.

Free Research Field

形成外科

Academic Significance and Societal Importance of the Research Achievements

透析患者における難治性創傷は重症化しやすく、かつ治療抵抗性で、長期の入院や外来通院、複数回の外科的治療を要することが多い。時には大切断や敗血症に至る場合もあり、よりよい創傷治癒を目指すことは重要な課題である。本研究ではAMSCの局所投与によって、Klothoマウスを用いた透析創傷モデルにおける創傷治癒への影響を検証することを目指した。透析創傷におけるAMSCの有効性を証明できれば、今後の新規薬剤開発に繋がる可能性がある。