Analysis of the role of Fam20C-mediated phosphorylation in mineralized tissues using Fam20C and Dmp1/Dspp overexpression mice

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K16930
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 57010:Oral biological science-related
• Research Institution: Osaka University
• Principal Investigator: Hirose Katsutoshi 大阪大学, 大学院歯学研究科, 助教 (00824898)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: 骨 / 象牙質 / 酸性リン蛋白質 / Fam20C / Dmp1 / Dspp / トランスジェニックマウス / リン酸化

Outline of Final Research Achievements

Family with sequence similarity 20, member C (FAM20C) is highly expressed in mineralized tissues, and phosphorylates an acidic phosphoproteins. Dentin matrix protein 1 (DMP1) is one of the chief acidic phosphoproteins in bone and dentin, and plays an important role for biomineralization. These findings suggest the role of FAM20C-mediated phosphorylation in their homeostasis. However, the role of FAM20C-mediated phosphorylation in bone tissues and tooth dentin remains unclear. Here, we investigated the role of FAM20C-mediated phosphorylation in mineralized tissue formation using osteoblast/odontoblast-specific Fam20C overexpression mice and osteoblast/odontoblast-specific Dmp1 overexpression mice. This study revealed that FAM20C-mediated phosphorylation is involved in mineralized tissue formation.

Free Research Field

骨

Academic Significance and Societal Importance of the Research Achievements

生体硬組織に特異的かつ豊富に存在する酸性リン蛋白質のリン酸化は、硬組織形成に重要な役割を担っていることが明らかとなった。「リン酸化のコントロール」は、骨折治療、直接歯髄覆髄法での新規硬組織誘導、象牙質知覚過敏症、初期う蝕の再石灰化療法などの新規治療ターゲットと考えられた。