Analysis of 53BP1 foci using super-resolution microscopy in response to radiation-induced DNA double-strand breaks

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K17888
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 63020:Radiation influence-related
• Research Institution: Keio University (2023); Gunma University (2021-2022)
• Principal Investigator: Uchihara Yuki 慶應義塾大学, 薬学部(芝共立), 助教 (10883712)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Keywords: DNA修復 / 53BP1 / 超高解像度解析 / DNA二本鎖切断 / クロマチン動態

Outline of Final Research Achievements

It has been reported that 53BP1 foci are composed of 53BP1 nanodomains at radiation-induced DNA double-strand break (DSB) sites using super-resolution fluorescence microscopy. In this study, we developed an experimental system to measure the distance between 53BP1 nanodomains in three dimensions (DMS: Distance from the Median Spot) using a super-resolution fluorescence microscope 3D-SIM (OMX). Using this experimental system, we analyzed the changes in DMS over time after irradiation and found that the distance between 53BP1 nanodomains is maintained regardless of the time after DSB damage.

Free Research Field

シグナル伝達

Academic Significance and Societal Importance of the Research Achievements

DNA二本鎖切断部位 (DSB) に集積する53BP1は、DSBを中心として左右に約1~2 Mb分布するが、その意義は明らかとなっていない。本研究において構築された実験系および成果は、未だ多くが未知である53BP1の機能解明やゲノム安定性維持機構に関わる知見に貢献すると考えている。