Secretory Characteristics and Functions of Exercise EVs for the Creation of Innovative Exercise Medicine

2022 Fiscal Year Final Research Report

• Project/Area Number: 21K18848
• Research Category: Grant-in-Aid for Challenging Research (Exploratory)
• Allocation Type: Multi-year Fund
• Review Section: Medium-sized Section 27:Chemical engineering and related fields
• Research Institution: Nagoya University
• Principal Investigator: Honda Hiroyuki 名古屋大学, 工学研究科, 教授 (70209328)
• Co-Investigator(Kenkyū-buntansha): 清水 一憲 名古屋大学, 工学研究科, 准教授 (70402500)
• Project Period (FY): 2021-07-09 – 2023-03-31
• Keywords: 細胞外小胞 / 電気刺激 / 骨格筋細胞 / 運動モデル

Outline of Final Research Achievements

It is not clear how exercise alters the number and quality of EVs secreted by skeletal muscle cells. In this study, we used an in vitro exercise model to examine whether exercise affects the amount of EVs released from skeletal muscle cells. The effects of 1 Hz and 30 Hz electrical stimulation applied under different exercise conditions for 24 hours were examined. The results showed that the amount of EVs secreted increased and the type of mircoRNAs contained in EVs also fluctuated when the subjects were exercised with 30 Hz electrical stimulation. Furthermore, it was suggested that changes in Alix expression and calcium ion concentration were involved in this.

Free Research Field

生物化学工学

Academic Significance and Societal Importance of the Research Achievements

本研究では培養骨格筋細胞への電気刺激によるin vitro運動モデルを用いて、in vivoでは困難である運動時の骨格筋細胞からのEVs分泌量が増加し、含まれるmicroRNAの種類も変化するを明らかにした。今後、分泌されたEVsの特性をさらに解析することで、運動効果の分子メカニズムが解明され、健康長寿社会の実現に貢献できる可能性がある。