Comprehensive explore for morphokinetic biomarkers expressed in association with chromosomal abnormalities in fertilized embryos

2022 Fiscal Year Final Research Report

• Project/Area Number: 21K19184
• Research Category: Grant-in-Aid for Challenging Research (Exploratory)
• Allocation Type: Multi-year Fund
• Review Section: Medium-sized Section 42:Veterinary medical science, animal science, and related fields
• Research Institution: Tokyo University of Agriculture and Technology
• Principal Investigator: Satoshi Sugimura 東京農工大学, (連合)農学研究科(研究院), 教授 (00728454)
• Project Period (FY): 2021-07-09 – 2023-03-31
• Keywords: 染色体異数性 / 形態動態 / 受精卵 / ウシ

Outline of Final Research Achievements

In this study, by analyzing the relationship between the developmental kinetics of bovine IVF embryos and chromosomal aneuploidy in blastocysts, I try to identify for biomarkers that predict chromosomal abnormalities. As a result, no relationship between morphokinetics such as pronuclear morphological dynamics, first cleavage patern, and duration of each cell cycle and chromosomal aneuploidy in blastocysts was observed. As a result of live cell imaging, the relationship between abnormal chromosomal segregation at early cleavage and developmental kinetics was demonstrated. Hence, it was suggested that morphokinetics are related to chromosomal aneuploidy in early cleavage rather than to blastocysts. In the future, it will be necessary to search for biomarkers other than morphokinetics related to chromosomal aneuploidy in blastocysts.

Free Research Field

動物生殖学

Academic Significance and Societal Importance of the Research Achievements

本研究成果は、哺乳動物の発生カイネティクスと染色体異数性との関係性を理解するうえで重要な知見となる。また、本研究成果は家畜生産のみならず、生殖補助医療といった医学分野への応用が期待される。