Study of the molecular mechanism that controls the plasmodesmata permeability in plants

2022 Fiscal Year Final Research Report

• Project/Area Number: 21K19247
• Research Category: Grant-in-Aid for Challenging Research (Exploratory)
• Allocation Type: Multi-year Fund
• Review Section: Medium-sized Section 44:Biology at cellular to organismal levels, and related fields
• Research Institution: Hokkaido University
• Principal Investigator: Fujita Tomomichi 北海道大学, 理学研究院, 教授 (50322631)
• Project Period (FY): 2021-07-09 – 2023-03-31
• Keywords: 原形質連絡 / ヒメツリガネゴケ

Outline of Final Research Achievements

In order to elucidate the molecular mechanism of a novel PD regulatory system independent of callose accumulation, we (a) conducted a screening of 2000 chemical compounds in the moss and (b) searched for a list of substrates of SnRK2 and a list of proteins localized to PD. Based on these results, we identified several candidate proteins and analyzed them. As a result, we found that one of the actin-related proteins, D, is involved in the regulation of PD. We are currently continuing our functional analysis of protein D and aim to elucidate the whole picture of the novel PD regulatory system by D.

Free Research Field

植物科学

Academic Significance and Societal Importance of the Research Achievements

PDは細胞壁を貫く小さな穴であり、様々な情報分子が移動している。近年、細胞壁多糖の1種、カロースの合成と分解の制御がこうした情報分子の細胞間移動に重要であることがわかってきた。この一方で、カロース以外にも重要なPD制御系があると考えられてはいるものの、その分子機構は未だによくわかっていない。本研究によりこの未知の制御系に関与する候補タンパク質を同定てきた可能性は高く、この学術的意義は大きい。今後さらに解析を進めることで、カロース非依存的なPD制御系の分子機構の解明が期待できる。