Establishment of rapid functional diagnosis of genome information and its application to cancer precision medicine

2023 Fiscal Year Final Research Report

• Project/Area Number: 21K19444
• Research Category: Grant-in-Aid for Challenging Research (Exploratory)
• Allocation Type: Multi-year Fund
• Review Section: Medium-sized Section 52:General internal medicine and related fields
• Research Institution: Shiga University of Medical Science
• Principal Investigator: Daigo Yataro 滋賀医科大学, 医学部, 教授 (30345029)
• Project Period (FY): 2021-07-09 – 2024-03-31
• Keywords: ゲノム機能診断 / がんプレシジョン医療

Outline of Final Research Achievements

To establish a foundation for developing qualitative diagnostic methods for genomic information through rapid functional evaluation of various genetic abnormalities identified in large-scale cancer genome analysis, we developed various gene regulation technologies using cancer-related genes as molecular models. We investigated assays to rapidly control gene function in human cell lines and detect this function in the cells. In order to construct a rapid functional evaluation system for various genetic changes and a foundation for diagnostic and therapeutic applications, we examined cell phenotypes and explored downstream molecules of the cancer-related genes under conditions in which the gene functions are controlled in human cell lines. We also developed a method to detect systematic changes in cell function caused by the cancer-related genes. We reported the results of this study at various conferences and published some manuscripts in international academic journals.

Free Research Field

分子腫瘍学

Academic Significance and Societal Importance of the Research Achievements

本研究で開発する遺伝子異常の高速機能評価技術は、複数の遺伝子異常の蓄積で生ずる発癌のメカニズムの解明と、その情報に基づいた癌診断・治療に向けた開発の迅速化に貢献するのみならず、他の生命科学分野にも応用可能と考えられる。例えば、この技術を用いた遺伝子異常の高速機能診断が可能になれば、癌の治療薬適用率を向上させることが可能になる。また、この手法は、他の医薬品開発技術と組み合わせることで、癌のみならず各種の疾患の治療法の開発につながる可能性がある。本研究成果は、新しい生命科学や医療開発に貢献でき、基礎研究のブレークスルーのみならず、その多分野への展開性が期待される。