2022 Fiscal Year Final Research Report
Reproduction of kidney glomerulus structure and function using human iPS cells-derived organoid and vascular chip
| Project/Area Number |
21K19487
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Review Section |
Medium-sized Section 53:Organ-based internal medicine and related fields
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| Research Institution | University of Miyazaki |
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Principal Investigator |
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| Project Period (FY) |
2021-07-09 – 2023-03-31
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| Keywords | オルガノイド / 腎糸球体 / 血管灌流 / 微小流体デバイス |
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| Outline of Final Research Achievements |
This study aimed to develop a method which enables to reproduce the higher order structure and function of kidney glomerulus ex vivo by using an integrated techniques that include microfluidic device, rotating culture and organoid creation. Both vascularized organoid with perfusion and rotating culture induced vascular invasion into the glomerulus structure of iPS cell derived kidney organoid at some extent, however it was not sufficient to induce mature glomerulus structure and vasculature. These suggest that additional technical development to reproduce kidney glomerulus structure and function.
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| Free Research Field |
血管生物医学
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| Academic Significance and Societal Importance of the Research Achievements |
血管化・灌流や回転培養という新しい方法論は，オルガノイド糸球体内の血管侵入という，腎糸球体・糸球体血管網の成熟化誘導ために重要な最初のステップの達成に有用であることを示した．更なる技術革新の足がかりとなる基盤データを提供している点において，学術的および社会的意義は大きい．
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