Proteome analysis of serum proteins adsorbed on hydroxyapatite with controlled crystal orientation

2022 Fiscal Year Final Research Report

• Project/Area Number: 21K19890
• Research Category: Grant-in-Aid for Challenging Research (Exploratory)
• Allocation Type: Multi-year Fund
• Review Section: Medium-sized Section 90:Biomedical engineering and related fields
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: Kawashita Masakazu 東京医科歯科大学, 生体材料工学研究所, 教授 (70314234)
• Co-Investigator(Kenkyū-buntansha): 横井 太史 東京医科歯科大学, 生体材料工学研究所, 准教授 (00706781)
• Project Period (FY): 2021-07-09 – 2023-03-31
• Keywords: 水酸アパタイト / 血清タンパク質 / プロテオーム解析 / 結晶配向性 / 吸着

Outline of Final Research Achievements

We found that 38, 33, and 24 serum proteins adsorbed on Hydroxyapatite powders orientated to the c-axis (m-HAp), those oriented to the a(b)-axis (c-HAp), and commercially available hydroxyapatite powders without crystal orientation control (i-HAp), respectively. Among them, the proteins that did not adsorb on i-HAp but adsorbed on m-HAp or c-HAp were selected, and their biological functions were investigated through a literature search. As a result, five kinds of proteins (vitamin D binding protein, transthyretin, ryanodine receptor 2, apolipoprotein E, and keratin) were selected as candidate proteins that are related with osteoconductivity of hydroxyapatite. In addition, it was found that there are some differences among m-HAp, c-HAp and i-HAp in metabolic pathway analysis by PANTHER and cell component classification by Gene Ontology.

Free Research Field

生体材料学

Academic Significance and Societal Importance of the Research Achievements

水酸アパタイトの骨結合性発現機構に関する従来研究の多くは細胞レベルでの検討・議論であり、本研究のように原子（結晶構造）・分子（タンパク質）レベルから水酸アパタイトの骨結合機構の解明に挑む研究はほぼ皆無である。今後検討すべき課題は数多いが、水酸アパタイトの骨結合性発現に関与するタンパク質候補として5種類のタンパク質が見出された点において、本研究を遂行した学術的・社会的意義は十分にある。また、本研究の成果は、生体－バイオマテリアルの界面科学に重要な知見を与え、生体材料学・プロテオミクス・医歯学の異分野融合型学問領域の創出に寄与する可能性がある。