Search and functional analysis of factors involved in the high temperature response of rice pollen formation

2022 Fiscal Year Final Research Report

• Project/Area Number: 21K20588
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Multi-year Fund
• Review Section: 0602:Agricultural and environmental biology and related fields
• Research Institution: National Agriculture and Food Research Organization
• Principal Investigator: Sagehashi Yoshiyuki 国立研究開発法人農業・食品産業技術総合研究機構, 生物機能利用研究部門, 上級研究員 (20414617)
• Project Period (FY): 2021-08-30 – 2023-03-31
• Keywords: 雄性不稔 / イネ / 温度感受性 / 花粉形成 / 高温感受性期 / 相互作用

Outline of Final Research Achievements

In this study, I aimed to clarify the function of a novel temperature-sensitive male sterility factor identified from rice, and to search for factors that interact with the novel factor. My future goal is to elucidate the mechanism by which pollen formation is inhibited at high temperature. First, I attempted to estimate the temperature-sensitive period of temperature sensitive male sterility lines and found that high temperature during the period from 12 to 14 days before ear emergence are important for the male sterility trait. I am now preparing a cDNA library for yeast two-hybrid by extracting RNA from anthers of rice plants including this period.

Free Research Field

作物生理学

Academic Significance and Societal Importance of the Research Achievements

本研究で温度感受性雄性不稔系統の高温感受性期を推定できたことは、温度感受性雄性不稔系統における高温条件下での花粉形成不稔のメカニズム解明のための有力な情報となる。同時に本研究において対象とした温度感受性雄性不稔をハイブリッド育種に利用する際の温度処理の時機に重要である。この高温感受性期に取得済みの新規温度感受性雄性不稔因子と相互作用する因子を明らかにすることで花粉形成不稔のメカニズム解明につながる。