2022 Fiscal Year Final Research Report
Functional Analysis of multidrug efflux pumps in Streptococcus pneumonaie
| Project/Area Number |
21K20759
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Multi-year Fund |
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| Review Section |
0803:Pathology, infection/immunology, and related fields
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2021-08-30 – 2023-03-31
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| Keywords | 肺炎球菌 / 薬剤耐性 / 薬剤排出ポンプ / 膜輸送体 / ABCトランスポーター |
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| Outline of Final Research Achievements |
In this study I constructed an efflux pump overexpression library in Streptococcus pneumoniae and tested this library against a variety of antimicrobial compounds to identify efflux pumps that are directly involved in antibiotic resistance. We identified several of these pumps of which one efflux pump turned out to be a previously uncharacterized ABC transporter. I purified this transporter to biochemically examine its activity and succeeded in measuring ATPase activity in vitro. I plan to structurally characterize this transporter in order to elucidate the mechanism of substrate transport.
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| Free Research Field |
微生物学
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| Academic Significance and Societal Importance of the Research Achievements |
肺炎球菌は先進国の死因の中核を占める肺炎の原因菌として広く認知されており、近年多剤耐性を持った肺炎球菌の蔓延が臨床現場において大きな問題となっている。多剤耐性肺炎球菌感染症に対する治療法を確立させるためには薬剤耐性機構の解明及ひ新規抗菌剤の標的の発見が喫緊の課題となっている。今回の研究は肺炎球菌の膜輸送体がどのように抗菌薬耐性に関与しているかを解明しており、本研究成果は革新的な感染症治療法の開発に応用できることが期待できる。
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