Development of a system to diagnose diverse host factor dependence by analogous viruses.

2022 Fiscal Year Final Research Report

• Project/Area Number: 21K20760
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Multi-year Fund
• Review Section: 0803:Pathology, infection/immunology, and related fields
• Research Institution: Osaka University
• Principal Investigator: Taguwa Shuhei 大阪大学, 感染症総合教育研究拠点, 特任准教授(常勤) (20513493)
• Project Period (FY): 2021-08-30 – 2023-03-31
• Keywords: Hsp70 / DnaJ / フラビウイルス

Outline of Final Research Achievements

Infectious viruses were created from DNA fragments in a simple manner. Arrayed libraries of fluorescent protein-fused PQCs, specifically GFP-fused Hsp70 (14 species), DnaJ (50 species), and NEF (15 species) were individually expressed. The established chaperone/co-chaperone expressing cells were infected with flaviviruses, respectively, to confirm the establishment of a system that can track the subcellular localization of chaperones due to virus infection, especially changes to the viral growth sites (protein synthesis, RNA replication, particle formation) over time.

Free Research Field

ウイルス学

Academic Significance and Societal Importance of the Research Achievements

世界で病原性を示すほぼ全てのフラビウイルスを網羅することに成功した。また異常蛋白質により凝集するシャペロンおよびコシャペロンを同定できるシステムも樹立した。ウイルスのみならず癌や神経変性疾患の病態解明にもつながる貴重なリソースである。