2022 Fiscal Year Final Research Report
Development of a Novel Diagnostic Method for Renal Disease by Profilings of Modified Nucleosides in Urine
Project/Area Number |
21K20925
|
Research Category |
Grant-in-Aid for Research Activity Start-up
|
Allocation Type | Multi-year Fund |
Review Section |
0903:Organ-based internal medicine and related fields
|
Research Institution | Kumamoto University |
Principal Investigator |
Nagayoshi Yu 熊本大学, 大学院生命科学研究部(医), 特任助教 (60908028)
|
Project Period (FY) |
2021-08-30 – 2023-03-31
|
Keywords | 修飾ヌクレオシド / バイオマーカー / 腎疾患 |
Outline of Final Research Achievements |
RNA has diverse chemical modifications over 100 types. We found that the modified RNA is degraded into single nucleosides and modified nucleosides are excreted into urine. However, the physiological mechanisms of this excretion and the relationships between renal diseases remain unclear. In this study, we investigated the effects of renal stress, mainly glomerular and tubular diseases, on the excretion of modified nucleosides using animal model and clinical specimens. The results of this study revealed that the excretion profiling of modified nucleosides is altered in mouse models of salt loading, diabetes, and tubular disorders, as well as in clinical samples.
|
Free Research Field |
腎臓内科
|
Academic Significance and Societal Importance of the Research Achievements |
これまでに腎疾患における修飾ヌクレオシド排泄変化は、未開拓の研究領域であり、今回の結果は新しい腎生理機能を明らかにするきっかけとなることから学術的意義は高い。加えて現在の臨床現場では、腎疾患の確定診断には腎生検を行う。腎生検は侵襲度の高い検査であるため、患者負担のみならず医療者の負担も大きい。本研究において明らかとなった、腎疾患モデルで排泄変化を認めた修飾ヌクレオシドは新規バイオマーカーとなる可能性がある。この結果は迅速簡便な新規腎疾患診断法に寄与する可能性が高く社会的意義も大きい。
|