Impact of the effects of stem cells on myocardial repair using intravital imaging techniques

2022 Fiscal Year Final Research Report

• Project/Area Number: 21K20949
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Multi-year Fund
• Review Section: 0905:Surgery of the organs maintaining homeostasis and related fields
• Research Institution: Osaka University
• Principal Investigator: Matsuura Ryohei 大阪大学, 大学院医学系研究科, 助教 (10768922)
• Project Period (FY): 2021-08-30 – 2023-03-31
• Keywords: intravital imaging

Outline of Final Research Achievements

We created a mouse model of myocardial infarction and constructed a system for intravital imaging of the mouse heart under general anesthesia. In the near future, we will evaluate cardiac function by dividing the mice into two groups: a sheet-group and a control group. After that, we plan to evaluate cardiac function in the sheet-group and the control group, and then explant the hearts, collect myocardium (3 sections each of infarct-, border-, and non-infarct regions), prepare tissue samples, and perform PCR/ELISA and histopathological evaluations. This system will be used to evaluate the mechanism of action and efficacy of regenerative medicine and other therapies. This system has the potential to be used as a drug screening method, to evaluate the mechanism of action and efficacy of therapies including regenerative medicine.

Free Research Field

心臓血管外科

Academic Significance and Societal Importance of the Research Achievements

心筋梗塞モデルマウスを作成し、全身麻酔下にマウス心臓のintravital imagingを撮影するシステムを構築した。このシステムを使って、再生医療を始めとした治療の作用機序や効果の評価を行うなど、ドラッグスクリーニング方法となる可能性が考えられる。