Collection of Basic Data and Dissemination of Messages for Clinical Applications of Dental Stem Cell Banks

2022 Fiscal Year Final Research Report

• Project/Area Number: 21K21025
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Multi-year Fund
• Review Section: 0907:Oral science and related fields
• Research Institution: The Nippon Dental University
• Principal Investigator: Shimamura Naohiro 日本歯科大学, 生命歯学部, 助教 (40908125)
• Project Period (FY): 2021-08-30 – 2023-03-31
• Keywords: 神経再生 / 紫外線 / 乾燥 / ツバメの巣 / Insert / 環境保護 / 三次元上皮培養 / 三次元神経細胞培養

Outline of Final Research Achievements

Enzyme-digested edible bird's nest (EBND) was found to have a cell-protective effect against oxidative stress, cell death, DNA double-strand breaks, and inflammation reactions caused by a combination of dry environment and UV exposure. EBND partially reduced inflammatory reactions in human HaCaT keratinocyte three-dimensional epithelial cultures, suggesting its potential to protect nerve cells and contribute to future treatments for nerve damage. The experiments also developed an environmentally friendly cell culture insert that provides an in vivo-like microenvironment facilitating cell-cell interactions and cytokine diffusion, which can reduce plastic waste at a low cost. These results are promising for three-dimensional nerve cell culture for clinical applications.

Free Research Field

細胞培養

Academic Significance and Societal Importance of the Research Achievements

本研究は、実現可能な再生医療として、DPSCsから三次元神経細胞への分化を誘導し、神経損傷に対する原因療法の確立へ向けた基礎データの収集を行い、再生医療の基盤を作るものである。また、本研究は若年期に保存した自分の歯の細胞を、未来の疾患罹患時に再利用するコンセプト“細胞バンク・テーラーメイド再生医療”の1モデルとなる可能性がある。本研究成果を発信することは、歯髄の特性や有用性を社会へ向けたメッセージとして発信でき、再生医療の新たな選択肢として歯髄細胞を臨床応用していく道筋ともなる。