Efficacy of lactbaciillus species in inhibiting Helicobacter pylori colonization using caries-induced rat models.

2022 Fiscal Year Final Research Report

• Project/Area Number: 21K21037
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Multi-year Fund
• Review Section: 0907:Oral science and related fields
• Research Institution: Osaka University
• Principal Investigator: Kadota Tamami 大阪大学, 歯学部附属病院, 助教 (10908643)
• Project Period (FY): 2021-08-30 – 2023-03-31
• Keywords: ヘリコバクター・ピロリ菌 / 乳酸菌 / ミュータンスレンサ球菌 / う蝕

Outline of Final Research Achievements

Oral specimens were obtained from 204 subjects, with Helicobacter pylori organisms detected in 61, while teeth extracted from 38 subjects were found to be affected by caries into the dentin. Furthermore, 16S metagenomic analysis showed that the detection rate of Lactobacillus delbrueckii tended to be higher in subjects with no caries and H. pylori not detected in oral specimens. Also, the number of colonies formed by Streptococcus mutans, L. delbrueckii, and H. pylori cultured in mitis-salivarius bacitracin agar medium was lower than the number formed by S. mutans alone.
The present findings indicate that L. delbrueckii inhibits formation of biofilms promoted by coexisting S. mutans and H. pylori, as well as the growth of S. mutans. It is possible that H. pylori is transmitted to the stomach via caries cavity induced by S. mutans, thus this study may contribute to not only strategies for dental caries prevention, but also inhibition and prevention of H. pylori colonization.

Free Research Field

口腔科学

Academic Significance and Societal Importance of the Research Achievements

本研究で得られた知見は、Lactobacillus delbrueckii がミュータンスレンサ球菌とピロリ菌の共存により促進されるバイオフィルムの形成や、ミュータンスレンサ球菌の増殖を抑制できる可能性を示すことができたという点に学術的意義を有するといえる。また、ピロリ菌はミュータンスレンサ球菌により誘発されるう窩を介して胃への感染が成立する可能性を有することから、これらの知見はう蝕予防だけでなく、ピロリ菌の定着抑制や定着予防にもつながると考えられ、L. delbrueckii の応用がう蝕予防とピロリ菌感染予防の両方に寄与できる可能性を有することを示した点に社会的意義があると考えられる。