2023 Fiscal Year Final Research Report

Analysis of molecular characteristics and metastatic mechanism of Pancreatic neuroendocrine neoplasm.

Research Project

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Project/Area Number	21KK0146
Research Category	Fund for the Promotion of Joint International Research (Fostering Joint International Research (B))
Allocation Type	Multi-year Fund
Review Section	Medium-sized Section 53:Organ-based internal medicine and related fields
Research Institution	Tokyo Medical and Dental University
Principal Investigator	Ito Go 東京医科歯科大学, 高等研究院, 特任助教 (20733900)
Co-Investigator(Kenkyū-buntansha)	小林正典東京医科歯科大学, 大学院医歯学総合研究科, 助教 (10825459) 工藤篤東京医科歯科大学, 医学部附属病院, 准教授 (20376734) 田邉稔東京医科歯科大学, 大学院医歯学総合研究科, 教授 (50197513) 岡本隆一東京医科歯科大学, 大学院医歯学総合研究科, 教授 (50451935)
Project Period (FY)	2021-10-07 – 2024-03-31
Keywords	膵神経内分泌腫瘍 / 肝転移 / 膵オルガノイド / 網羅的遺伝子発現解析 / RNA-seq
Outline of Final Research Achievements	In this international collaborative study, we aimed to clarify the molecular biological mechanisms that define the metastasis of NEN by using the combination of advanced high throughput technology of the collaborating institution in Germany, and the abundant number of NEN cases and organoid technology of our research group, which is one of the best in Japan.We performed RNA-seq using surgical specimens from primary lesions, liver metastases, normal pancreas tissues, and organoids established from the same specimens. As a results, we identified candidate genes that regulate metastasis by comparing gene expression at each sample. In addition, we established an international exchange agreement between Kiel University and Tokyo medical and dental university, which will lead to the further development of international exchange and collaboration works.
Free Research Field	消化器病態学
Academic Significance and Societal Importance of the Research Achievements	本研究により膵神経内分泌腫瘍の悪性度・転移を規定する遺伝子群の同定に成功しており、pan-NENの分子生物学的特性を解明する新たな基盤となる知見が獲得された。またpan-NEN手術検体・オルガノイドバイオバンクの樹立により今後の創薬や新薬の治療効果判定などへと利用可能な解析系の構築も実施している。今後pan-NENの臨床的特性をより正確に反映する新たな分類の作成などpan-NEN診断・治療につながると考えている。また今後のドイツとの学術上の国際交流の基盤を作りさらなる発展が期待される。