Creation of genome manipulation technology using super restriction enzyme

2015 Fiscal Year Final Research Report

• Project/Area Number: 22000007
• Research Category: Grant-in-Aid for Specially Promoted Research
• Allocation Type: Single-year Grants
• Review Section: Science and Engineering; Chemistry
• Research Institution: University of Tsukuba (2011-2015); The University of Tokyo (2010)
• Principal Investigator: KOMIYAMA Makoto 筑波大学, 生命領域学際研究センター, 教授 (50133096)
• Co-Investigator(Kenkyū-buntansha): SUMAOKA Jun 筑波大学, 生命領域学際研究センター, 講師 (10280934) ; XU Yan 宮崎大学, 医学部, 教授 (40506763) ; ITO Yasuhiro 東京大学, 分子細胞生物学研究所, 助教 (50508108) ; HIGUCHI Maiko 東京大学, 分子細胞生物学研究所, 助教 (30420235)
• Co-Investigator(Renkei-kenkyūsha): SHIGI Narumi 筑波大学, 生命領域学際研究センター, 研究員 (00396780) ; AIBA Yuichiro 筑波大学, 生命領域学際研究センター, 研究員 (10581085)
• Project Period (FY): 2010 – 2015
• Keywords: DNA / 制限酵素 / ペプチド核酸 / 遺伝子組換え / セリウム

Outline of Final Research Achievements

Man-mode tool (super artificial restriction enzyme), developed by our group, is highly site-selective and cuts huge human genome at one target site. In this research, this cutter was used to obtain desired fragments from human genome. Telomeres and other fragments were clipped from human genome with high selectivity. It was found that the telomere length is different from a chromosome to another even in the same cells. This unexpected finding has never been obtained by previous methods, showing superb roles of the present chemical cutter for molecular biology. Even in alive human cells, the super artificial restriction enzyme site-selectively cut the genome, inducing desired homologous recombination. Furthermore, the function of the cutters were improved by various chemical modifications. Attachment of nuclear localization signal peptide to PNA was especially effective and allowed the invasion of the PNA into the genome to proceed spontaneously under physiological conditions.

Free Research Field

化学