生殖制御における新規脳内分子機構の解明

2012 Fiscal Year Annual Research Report

• Project/Area Number: 22227002
• Research Institution: Waseda University
• Principal Investigator: 筒井 和義 早稲田大学, 教育・総合科学学術院, 教授 (20163842)
• Co-Investigator(Kenkyū-buntansha): 緒方 勤 浜松医科大学, 医学部, 教授 (40169173)
• Project Period (FY): 2010-05-31 – 2015-03-31
• Keywords: 視床下部ホルモン / 脳下垂体ホルモン / 発現制御 / シグナル伝達 / 生殖 / 生殖行動 / 生殖機能異常 / 分子進化

Research Abstract

本年度に以下の主たる成果を得た
「GnIH生殖抑制作用の分子機構の解析」：GnIHは生殖腺刺激ホルモン産生細胞においてcAMP合成を低下させ、ERKのリン酸化を阻害して生殖腺刺激ホルモンの合成を抑制することを明らかにした。GnIHはGnRHニューロンにおいてcAMP合成を低下させてGnRHの合成を抑制することを明らかにした。「GnIH発現の分子制御機構の解析」：メラトニンとグルココルチコイドがGnIHの発現を高め、生殖制御鍵遺伝子DIO2が合成する活性型甲状腺ホルモンがGnIHの発現を低下させることを明らかにした。「生殖腺におけるGnIHの局所作用機構の解析」：GnIHは卵巣の顆粒膜細胞においてcAMP合成を低下させてプロゲステロン合成を減少させるというGnIHの新しい生殖抑制作用機構を発見した。「GnIHによる生殖行動の発現制御機構の解析」：GnIHは脳内のエストロゲン合成を変動させて生殖行動を制御するという新しい脳内分子制御機構を発見した「GnIH遺伝子のノックダウンによるGnIH生理作用の解析」：GnIH遺伝子のノックダウンにより、GnIHの生殖抑制作用を証明した。さらに、GnIHは動物の覚醒を抑えるという新しい生理作用を発見した。「GnIHによるヒト生殖機能異常の分子機構の解析」：生殖機能異常症患者のDNA検体を集積し、次世代シーケンサーによりGnIH遺伝子とGnIH受容体遺伝子の変異解析を進めた。「動物界におけるGnIHとGnIH受容体の起源と分子進化の解析」：GnIHとGnIH受容体は全ての脊椎動物に加えて無脊椎動物である原索動物（ナメクジウオ）にも存在することを明らかにし、動物界におけるGnIHとGnIH受容体の起源と分子進化に重要な知見を得た。本年度の研究より、「生殖制御における新規脳内分子機構」に関する重要な新知見が数多く得られた。

Current Status of Research Progress

1: Research has progressed more than it was originally planned.

Reason

事前の十分な準備と適切な研究計画により、当初の目的を超える研究の進展があり、GnIH生殖抑制作用の分子機構、GnIH発現の分子制御機構、生殖腺におけるGnIHの局所作用機構、GnIHによる生殖行動の発現制御機構などに重要な新知見が数多く得られた。また、GnIH遺伝子のノックダウンによりGnIHの生理作用が証明された。さらに、GnIH とGnIH受容体は全ての脊椎動物に加えて無脊椎動物である原索動物にも存在することが明らかとなり、動物界におけるGnIHとGnIH受容体の起源と分子進化に重要な知見が得られた。このように、予想以上の研究進展により、古典的な理解とは遥かにかけ離れた「生殖制御における新規脳内分子機構」の理解が進展した。

Strategy for Future Research Activity

今後の研究では、GnIH生殖抑制作用の分子機構、GnIH発現の分子制御機構、生殖腺におけるGnIHの局所作用機構、GnIHによる生殖行動の発現制御機構などを継続して解析する。また、GnIH遺伝子のノックアウトマウスを作製したので、本格的にGnIH作用の生理的意義とGnIHの新しい生理作用を解析する。生殖機能異常症患者のDNA検体が集積したので、次世代シーケンサーを用いて本格的にGnIH遺伝子とGnIH受容体遺伝子の変異解析を行う。GnIHとGnIH受容体の起源は原索動物に遡ることを明らかにしたので、他の無脊椎動物からGnIHとGnIH受容体を同定し、遺伝子のシンテニー解析を行う。全ての研究準備を終えており、今後の研究推進に問題は無い。予想を上回る研究の成果が見込まれる。

Research Products

• [Journal Article] Review: 7α-Hydroxypregnenolone, a new key stimulator of locomotor activity: biosynthesis, mode of action and functional significance. (2013)
  • Author(s): K. Tsutsui, S. Haraguchi, Y. Fukada, H. Vaudry.
  • Journal Title: Front. Neuroendocrinol. Volume: 印刷中 Pages: in press
  • Peer Reviewed
• [Journal Article] Review: Identification, localization and function of a novel neuropeptide, 26RFa, and its cognate receptor, GPR103 in the avian hypothalamus. (2013)
  • Author(s): K. Ukena, T. Tachibana, Y. Tobari, J. Leprince, H. Vaudry, K. Tsutsui.
  • Journal Title: Gen. Comp. Endocrinol. Volume: 印刷中 Pages: in press
  • DOI: 10.1016/j.ygcen.2013.03.014
  • Peer Reviewed
• [Journal Article] Editorial: 10th International Symposium on Avian Endocrinology. (2013)
  • Author(s): K. Tsutsui, P.J. Sharp, R.M. Dores.
  • Journal Title: Gen. Comp. Endocrinol. Volume: 印刷中 Pages: in press
  • DOI: 10.1016/j.ygcen.2013.03.011
  • Peer Reviewed
• [Journal Article] Review: Gonadotropin-inhibitory hormone (GnIH), GnIH receptor and cell signaling. (2013)
  • Author(s): T. Ubuka, Y.L. Son, G.E. Bentley, R.P. Millar, K. Tsutsui.
  • Journal Title: Gen. Comp. Endocrinol. Volume: 印刷中 Pages: in press
  • DOI: 10.1016/j.ygcen.2013.02.030
  • Peer Reviewed
• [Journal Article] A genetically female brain is required for a regular reproductive cycle in chicken brain chimeras. (2013)
  • Author(s): F. Maekawa, M. Sakurai, Y. Yamashita, K. Tanaka, S. Haraguchi, K. Yamamoto, K. Tsutsui et al.
  • Journal Title: Nature Communications Volume: 4 Pages: Art. No. 1372
  • DOI: 10.1038/ncomms2372
  • Peer Reviewed
• [Journal Article] Contribution of Leydig and Sertoli cells to testosterone production in mouse fetal testes. (2013)
  • Author(s): Y. Shima, K. Miyabayashi, S. Haraguchi, T. Arakawa, H. Otake, T. Baba, S. Matsuzaki, Y. Shishido, H. Akiyama, T. Tachibana, K. Tsutsui, K. Morohashi.
  • Journal Title: Mol. Endocrinol. Volume: 27 Pages: 63-73
  • DOI: 10.1210/me.2012-1256
  • Peer Reviewed
• [Journal Article] Review: Regulatory mechanisms of gonadotropin-inhibitory hormone (GnIH) synthesis and release in photoperiodic animals. (2013)
  • Author(s): K. Tsutsui, T. Ubuka, G.E. Bentley, L.J. Kriegsfeld.
  • Journal Title: Front. Neurosci. Volume: 3 Pages: Art. No. 126
  • DOI: 10.3389/fnins.2013.00060
  • Peer Reviewed
• [Journal Article] Review: Neuroendocrine regulation of gonadotropin secretion in seasonally breeding birds. (2013)
  • Author(s): T. Ubuka, G.E. Bentley, K. Tsutsui.
  • Journal Title: Front. Neurosci. Volume: 7 Pages: Art. No. 38
  • DOI: 10.3389/fnins.2013.00038
  • Peer Reviewed
• [Journal Article] Review: RNA interference of gonadotropin-inhibitory hormone gene induces aggressive and sexual behaviors in birds. (2013)
  • Author(s): T. Ubuka, T. Mizuno, Y. Fukuda, G.E. Bentley, J.C. Wingfield, K. Tsutsui.
  • Journal Title: Gen. Comp. Endocrinol. Volume: 181 Pages: 179-186
  • DOI: 10.1016/j.ygcen.2012.09.010
  • Peer Reviewed
• [Journal Article] Review: Melatonin stimulates the synthesis and release of gonadotropin-inhibitory hormone in birds. (2013)
  • Author(s): V.S. Chowdhury, T. Ubuka, K. Tsutsui.
  • Journal Title: Gen. Comp. Endocrinol. Volume: 181 Pages: 175-178
  • DOI: 10.1016/j.ygcen.2012.08.005
  • Peer Reviewed
• [Journal Article] Review: Create new research directions in comparative endocrinology from Asia and Oceania. (2013)
  • Author(s): K. Tsutsui.
  • Journal Title: Gen. Comp. Endocrinol. Volume: 181 Pages: 192-196
  • DOI: 10.1016/j.ygcen.2012.04.015
  • Peer Reviewed
• [Journal Article] 総説：松果体で合成・放出されたアロプレグナノロンは発達期の小脳プルキンエ細胞の生存に不可欠である. (2013)
  • Author(s): 原口省吾・原桜子・産賀崇由・三田雅敏・筒井和義
  • Journal Title: 比較内分泌学 Volume: 39 Pages: 44-48
  • Peer Reviewed
• [Journal Article] A report of two novel NR5A1 mutation families: possible clinical phenotype of psychiatric symptoms of anxiety and/or depression. (2013)
  • Author(s): A. Suzuki-Suwanai, T. Ishii, H. Haruna, A. Yamataka, S. Narumi, R. Fukuzawa, T. Ogata, T. Hasegawa.
  • Journal Title: Clin. Endocrinol. Volume: 印刷中 Pages: in press
  • DOI: 10.1111/cen.12054
  • Peer Reviewed
• [Journal Article] Mamld1 deficiency significantly reduces mRNA expression levels of multiple genes expressed in mouse fetal Leydig cells but permits normal genital and reproductive development. (2013)
  • Author(s): M. Miyado, M. Nakamura, K. Miyado, K. Morohashi, S. Sano, E. Nagata, M. Fukami, T. Ogata.
  • Journal Title: Endocrinology Volume: 印刷中 Pages: in press
  • DOI: 10.12010/en.2012-1324
  • Peer Reviewed
• [Journal Article] Possible contribution of fetal size and gestational age to myocardial tissue Doppler velocities in preterm fetuses. (2013)
  • Author(s): K. Sekii, H. Itoh, T. Ogata, S. Iwashima.
  • Journal Title: Eur. J. Obstet. Gynecol. Reprod. Biol. Volume: 印刷中 Pages: in press
  • DOI: 10.1016/j.ejogrb.2012.09.027
  • Peer Reviewed
• [Journal Article] Neuromuscular symptoms in a patient with familial pseudohypoparathyroidism type Ib diagnosed by methylation-specific multiplex ligation-dependent probe amplification. (2013)
  • Author(s): K. Nagasaki, S. Tsuchiya, A. Saitoh, T. Ogata, M. Fukami.
  • Journal Title: Endocr. J. Volume: 印刷中 Pages: in press
  • DOI: 10.1507/endocrj.EJ12-0257
  • Peer Reviewed
• [Journal Article] Glucose-6-phosphate dehydrogenase deficiency and adrenal hemorrhage in a Filipino neonate with hyperbilirubinemia. (2013)
  • Author(s): A. Ohishi, D. Ueno, T. Ogata.
  • Journal Title: Am. J. Perinatol. Reports Volume: 印刷中 Pages: in press
  • DOI: 10.1055/s-0032-1329125
  • Peer Reviewed
• [Journal Article] Molecular and clinical studies in 138 Japanese patients with Silver-Russell syndrome. (2013)
  • Author(s): T. Fuke, S. Mizuno, T. Nagai, T. Hasegawa, R. Horikawa, Y. Miyoshi, K. Muroya, T. Kondoh, C. Numakura, S. Sato, K. Nakabayashi, C. Tayama, K. Hata, S. Sano, K. Matsubara, T. Ogata. et al.
  • Journal Title: PLoS ONE Volume: 印刷中 Pages: in press
  • DOI: 10.1371/journal.pone.0060105
  • Peer Reviewed
• [Journal Article] Birth seasonality in Prader-Willi syndrome resulting from chromosome 15 microdeletion. (2013)
  • Author(s): T. Ayabe, K. Matsubara, T. Ogata, A. Ayabe, N. Murakami, T. Nagai, M. Fukami.
  • Journal Title: Am. J. Med. Genet. A Volume: 印刷中 Pages: in press
  • Peer Reviewed
• [Journal Article] Mutation and deletion of fibroblast growth factor receptor 1 gene in two patients with combined pituitary hormone deficiency. (2013)
  • Author(s): M. Fukami, M. Iso, N. Sato, M. Igarashi, M. Seo, I. Kazukawa, E. Kinoshita, S. Dateki, T. Ogata.
  • Journal Title: Endocr. J. Volume: 印刷中 Pages: in press
  • Peer Reviewed
• [Journal Article] A novel homozygous mutation of the nicotinamide nucleotide transhydrogenase gene in a Japanese patient with familial glucocorticoid deficiency. (2013)
  • Author(s): R. Yamaguchi, F. Kato, T. Hasegawa, N. Katsumata, M. Fukami, T. Matsui, K. Nagasaki, T. Ogata.
  • Journal Title: J. Hum. Genet. Volume: 印刷中 Pages: in press
  • DOI: 10.1507/endocrj.EJ13-0024
  • Peer Reviewed
• [Journal Article] Advanced maternal age at childbirth and the development of uniparental disomy. A commentary on the proportion of uniparental disomy is increased in Prader-Willi syndrome due to an advanced maternal childbearing age in Korea. (2013)
  • Author(s): K. Matsubara, T. Ogata.
  • Journal Title: J. Hum. Genet. Volume: 58 Pages: 118-119
  • DOI: 10.1038/jhg.2013.4
  • Peer Reviewed
• [Journal Article] Review: Backdoor pathway for dihydrotestosterone biosynthesis: implications for normal and abnormal human sex development. (2013)
  • Author(s): M. Fukami, K. Homma, T. Hasegawa, T. Ogata.
  • Journal Title: Dev Dyn. Volume: 242 Pages: 320-329
  • DOI: 10.1002/dvdy.23892
  • Peer Reviewed
• [Journal Article] Possible role of pineal allopregnanolone in Purkinje cell survival. (2012)
  • Author(s): S. Haraguchi, S. Hara, T. Ubuka, M. Mita, K. Tsutsui.
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 109 Pages: 21110-21115
  • DOI: 10.1073/pnas.1210804109
  • Peer Reviewed
• [Journal Article] Localization of gonadotropin-releasing hormone (GnRH), gonadotropin-inhibitory hormone (GnIH), kisspeptin and GnRH receptor and their possible roles in testicular activities from birth to senescence in mice. (2012)
  • Author(s): S. Anjum, A. Krishna, R. Sridaran, K. Tsutsui.
  • Journal Title: J. Exp. Zool. Part A Volume: 317 Pages: 630-644
  • DOI: 10.1002/jez.1765
  • Peer Reviewed
• [Journal Article] Involvement of the neurosteroid 7α-hydroxypregnenolone in the courtship behavior of the male newt, Cynops pyrrhogaster. (2012)
  • Author(s): F. Toyoda, I. Hasunuma, T. Nakada, S. Haraguchi, K. Tsutsui, S. Kikuyama.
  • Journal Title: Horm. Behav. Volume: 62 Pages: 375-380
  • DOI: 10.1016/j.yhbeh.2012.07.001
  • Peer Reviewed
• [Journal Article] Disrupted organization of RFamide pathways in the hypothalamus is associated with advanced puberty in female rats neonatally exposed to Bisphenol A. (2012)
  • Author(s): S.M. Losa-Ward, K.L. Todd, K.A. McCaffrey, K. Tsutsui, H.B. Patisaul.
  • Journal Title: Biol. Reprod. Volume: 87 Pages: 1-9
  • DOI: 10.1095/biolreprod.112.100826
  • Peer Reviewed
• [Journal Article] Effects of lamprey PQRFamide peptides on brain gonadotropin-releasing hormone concentrations and pituitary gonadotropin-β mRNA expression. (2012)
  • Author(s): D. Daukss, K. Gazda, T. Kosugi, T. Osugi, K. Tsutsui, S.A. Sower.
  • Journal Title: Gen. Comp. Endocrinol. Volume: 177 Pages: 215-219
  • DOI: 10.1016/j.ygcen.2012.04.024
  • Peer Reviewed
• [Journal Article] Hypothalamic gonadotropin-inhibitory hormone precursor mRNA is increased during depressed food intake in heat-exposed chicks. (2012)
  • Author(s): V.S. Chowdhury, S. Tomonaga, S. Nishimura, S. Tabata, J.F. Cockrem, K. Tsutsui, M. Furuse.
  • Journal Title: Comp. Biochem. Physiol. Part A Volume: 162 Pages: 227-233
  • DOI: 10.1016/j.cbpa.2012.03.009
  • Peer Reviewed
• [Journal Article] Evolutionary origin of the structure and function of gonadotropin-inhibitory hormone: Insights from lampreys. (2012)
  • Author(s): T. Osugi, T. Kosugi, T. Ubuka, K. Gazda, D. Daukss, M. Nozaki, S.A. Sower, K. Tsutsui.
  • Journal Title: Endocrinology Volume: 153 Pages: 2362-2374
  • DOI: 10.1210/en.2011-2046
  • Peer Reviewed
• [Journal Article] Mollusc gonadotropin-releasing hormone directly regulates gonadal functions: A primitive endocrine system controlling reproduction. (2012)
  • Author(s): N. Treen, N. Itoh, H. Miura, I. Kikuchi, T. Ueda, K.G. Takahashi, T. Ubuka, K. Yamamoto, P.J. Sharp, K. Tsutsui, M. Osada.
  • Journal Title: Gen. Comp. Endocrinol. Volume: 176 Pages: 167-172
  • DOI: 10.1016/j.ygcen.2012.01.008
  • Peer Reviewed
• [Journal Article] Gonadotropin-inhibitory hormone inhibits gonadotropin-releasing hormone-Induced gonadotropin synthesis mediated by adenylate cyclase/cAMP/PKA-dependent ERK pathway in LβT2 gonadotrope cells. (2012)
  • Author(s): Y.L. Son, T. Ubuka, R.P. Millar, H. Kanasaki, K. Tsutsui.
  • Journal Title: Endocrinology Volume: 153 Pages: 2332-2343
  • DOI: 10.1210/en.2011-1904
  • Peer Reviewed
• [Journal Article] Resveratrol promotes expression of SIRT1 and StAR in rat ovarian granulosa cells: an implicative role of SIRT1 in the ovary. (2012)
  • Author(s): Y. Morita, O. Wada-Hiraike, T. Yano, S. Nakagawa, A. Shirane, H. Hiraike, S. Koyama, M. Hirano, H. Oishi, O. Yoshino, Y. Miyamoto, K. Sone, K. Oda, K. Tsutsui, Y. Taketani.
  • Journal Title: Reprod. Biol. Endocrinol. Volume: 10 Pages: 14-24
  • DOI: 10.1186/1477-7827-10-14
  • Peer Reviewed
• [Journal Article] The human gonadotropin-inhibitory hormone ortholog RFamide-related peptide-3 suppresses gonadotropin-induced progesterone production in human granulosa cells. (2012)
  • Author(s): H. Oishi, C. Klausen, G.E. Bentley, T. Osugi, K. Tsutsui, C.B. Gilks, T. Yano, P.C.K. Leung.
  • Journal Title: Endocrinology Volume: 153 Pages: 3435-3445
  • DOI: 10.1210/en.2012-1066
  • Peer Reviewed
• [Journal Article] Developmental changes in the mammalian gonadotropin-inhibitory hormone (GnIH) ortholog RFamide-related peptide (RFRP) and its cognate receptor GPR147 in the rat hypothalamus. (2012)
  • Author(s): T. Iwasa, T. Matsuzaki, M. Murakami, R. Kinouchi, T. Osugi, G. Gereltsetseg, S. Yoshida, M. Irahara, K. Tsutsui.
  • Journal Title: Int. J. Dev. Neurosci. Volume: 30 Pages: 31-37
  • DOI: 10.1016/j.ijdevneu.2011.10.003
  • Peer Reviewed
• [Journal Article] RNA interference of gonadotropin-inhibitory hormone gene induces arousal in songbirds. (2012)
  • Author(s): T. Ubuka, M. Mukai, J. Wolfe, R. Beverly, S. Clegg, A. Wang, S. Hsia, M. Li, J.S. Krause, T. Mizuno, Y. Fukuda, K. Tsutsui, G.E. Bentley, J.C. Wingfield.
  • Journal Title: PLoS ONE Volume: 7 Pages: Art. No. e30202
  • DOI: 10.1371/journal.pone.0030202
  • Peer Reviewed
• [Journal Article] Acute stress increases the synthesis of 7α-hydroxypregnenolone, a new key neurosteroid stimulating locomotor activity, through corticosterone action. (2012)
  • Author(s): S. Haraguchi, T. Koyama, I. Hasunuma, S. Okuyama, T. Ubuka, S. Kikuyama, J.L. Do Rego, H. Vaudry, K. Tsutsui.
  • Journal Title: Endocrinology Volume: 153 Pages: 794-805
  • DOI: 10.1210/en.2011-1422
  • Peer Reviewed
• [Journal Article] Identification, expression, and physiological functions of Siberian hamster gonadotropin-inhibitory hormone. (2012)
  • Author(s): T. Ubuka, K. Inoue, Y. Fukuda, T. Mizuno, K. Ukena, L.J. Kriegsfeld, K. Tsutsui.
  • Journal Title: Endocrinology Volume: 153 Pages: 373-385
  • DOI: 10.1210/en.2011-1110
  • Peer Reviewed
• [Journal Article] Editorial: Research Topic on Neurosteroids. (2012)
  • Author(s): H. Vaudry, K. Tsutsui.
  • Journal Title: Front. Endocrinol. Volume: 3 Pages: Art. No. 126
  • DOI: 10.3389/fendo.2012.00126
  • Peer Reviewed
• [Journal Article] Review: Gonadotropin-inhibitory hormone action in the brain and pituitary. (2012)
  • Author(s): T. Ubuka, Y.L. Son, Y. Tobari, K. Tsutsui.
  • Journal Title: Front. Endocrinol. Volume: 3 Pages: Art. No. 148
  • DOI: 10.3389/fendo.2012.00148
  • Peer Reviewed
• [Journal Article] Review: Gonadotropin-inhibitory hormone (GnIH): discovery, progress and prospect. (2012)
  • Author(s): K. Tsutsui, T. Ubuka, G.E. Bentley, L.J. Kriegsfeld.
  • Journal Title: Gen. Comp. Endocrinol. Volume: 177 Pages: 305-314
  • DOI: 10.1016/j.ygcen.2012.02.013
  • Peer Reviewed
• [Journal Article] Review: Neurosteroid biosynthesis and action during cerebellar development. (2012)
  • Author(s): K. Tsutsui.
  • Journal Title: Cerebellum Volume: 11 Pages: 414-415
  • DOI: 10.1007/s12311-011-0341-7
  • Peer Reviewed
• [Journal Article] Review: Estradiol promotes Purkinje dendritic growth, spinogenesis and synaptogenesis during neonatal life by inducing the expression of BDNF. (2012)
  • Author(s): S. Haraguchi, K. Sasahara, H. Shikimi, S. Honda, N. Harada, K. Tsutsui.
  • Journal Title: Cerebellum Volume: 11 Pages: 416-417
  • DOI: 10.1007/s12311-011-0342-6
  • Peer Reviewed
• [Journal Article] Review: Control of circadian activity of birds by the interaction of melatonin with 7α-hydroxypregnenolone, a newly discovered neurosteroid stimulating locomotion. (2012)
  • Author(s): K. Tsutsui, S. Haraguchi, K. Inoue, H. Miyabara, S. Suzuki, T. Ubuka.
  • Journal Title: J. Ornitol. Volume: 153 Pages: S235-S243
  • DOI: 10.1007/s10336-011-0795-x
  • Peer Reviewed
• [Journal Article] Review: 7α-Hydroxypregnenolone, a new key regulator of amphibian locomotion: discovery, progress and prospect. (2012)
  • Author(s): K. Tsutsui, S. Haraguchi, M. Matsunaga, T. Koyama, J.L. Do Rego, H. Vaudry.
  • Journal Title: Gen. Comp. Endocrinol. Volume: 176 Pages: 440-447
  • DOI: 10.1016/j.ygcen.2011.11.020
  • Peer Reviewed
• [Journal Article] Review: Regulation of neurosteroid biosynthesis by neurotransmitters and neuropeptides. (2012)
  • Author(s): J.L. Do Rego, J.Y. Seong, D. Burel, J. Leprince, D. Vaudry, V. Luu-The, M.C. Tonon, K. Tsutsui, G. Pelletier, H. Vaudry.
  • Journal Title: Front. Endocrinol. Volume: 3 Pages: Art. No. 4
  • DOI: 10.3389/fendo.2012.00004
  • Peer Reviewed
• [Journal Article] 総説：生殖を制御する新規脳ホルモンの作用機構と発現制御機構 Current Topics｢New Insights of Molecular Genetics on Growth Disorders｣. (2012)
  • Author(s): 筒井和義.
  • Journal Title: ファイザー社 Kokua Volume: 7 Pages: 13
  • Peer Reviewed
• [Journal Article] 総説：生殖を制御する新規脳ホルモンGnIHの起源と分子進化. (2012)
  • Author(s): 筒井和義・大杉知裕・戸張靖子・孫ユリ・産賀崇由.
  • Journal Title: 比較内分泌学 Volume: 38 Pages: 76-86
  • Peer Reviewed
• [Journal Article] 総説：「ニューロペプチド-update」生殖腺刺激ホルモン放出抑制ホルモン. (2012)
  • Author(s): 筒井和義.
  • Journal Title: 中外医学社 Clinical Neuroscience Volume: 30 Pages: 223-225
  • Peer Reviewed
• [Journal Article] Identification of a novel mutation in the exon 2 splice donor site of the POU1F1/PIT-1 gene in Japanese identical twins with mild combined pituitary hormone deficiency. (2012)
  • Author(s): H. Inoue, T. Mukai, Y. Sakamoto, C. Kimura, N. Kangawa, M. Itakura, T. Ogata, Y. Ito, K. Fujieda.
  • Journal Title: Clin. Endocrinol. Volume: 76 Pages: 78-87
  • DOI: 10.1111/j.1365-2265.2011.04165.x
  • Peer Reviewed
• [Journal Article] Genetic characteristics on HLA-cass II and class I among Japanese type 1A and type 1B diabetic children and their families. (2012)
  • Author(s): S. Sugihara, T. Ogata, T. Kawamura, T. Urakami, K. Takemoto, N. Kikuchi, N. Takubo, K. Tsubouchi, R. Horikawa, K. Kobayashi, Y. Kasahara, T. Kikuchi, A. Koike, T. Mochizuki, et al.
  • Journal Title: Pediatr. Diabetes Volume: 13 Pages: 33-44
  • DOI: 10.1111/j.1399-5448.2011.00833.x
  • Peer Reviewed
• [Journal Article] Relative frequency of underlying genetic causes for the development of UPD(14)pat-like phenotype. (2012)
  • Author(s): M. Kagami, F. Kato, K. Matsubara, T. Sato, G. Nishimura, T. Ogata.
  • Journal Title: Eur. J. Hum. Genet. Volume: 20 Pages: 928-932
  • DOI: 10.1038/ejhg.2012.26
  • Peer Reviewed
• [Journal Article] Growth hormone secretion and its effect on height in pediatric patients with different genotypes of Prader-Willi syndrome. (2012)
  • Author(s): Y. Oto, K. Obata, K. Matsubara, Y. Kozu, T. Tsuchiya, S. Sakazume, A. Yoshino, N. Murakami, T. Ogata, T. Nagai.
  • Journal Title: Am. J. Med. Genet. A. Volume: 158A Pages: 1477-1480
  • DOI: 10.1002/ajmg.a35378
  • Peer Reviewed
• [Journal Article] Mosaic upd(7)mat in a patient with Silver-Russell syndrome: correlation between phenotype and mosaic ratio in the body and the placenta. (2012)
  • Author(s): T. Fuke-Sato, K. Yamazawa, K. Nakabayashi, K. Matsubara, K. Matsuoka, T. Hasegawa, K. Dobashi, T. Ogata.
  • Journal Title: Am. J. Med. Genet. A. Volume: 158A Pages: 465-468
  • DOI: 10.1002/ajmg.a.34404
  • Peer Reviewed
• [Journal Article] 46, XY gonadal dysgenesis: new SRY point mutation in two siblings with paternal germ line mosaicism. (2012)
  • Author(s): S. Stoppa-Vaucher, T. Ayabe, J. Paquette, N. Patey, D. Francoeur, J.M. Vuissoz, J. Deladoey, M.E. Samuels, T. Ogata, C.L. Deal.
  • Journal Title: Clin. Genet. Volume: 82 Pages: 505-513
  • DOI: 10.1111/j.1399-0004.2011.01832.x
  • Peer Reviewed
• [Journal Article] Prevalence and clinical features of Costello syndrome and cardio-facio-cutaneous syndrome in Japan: Findings from a nationwide epidemiological survey. (2012)
  • Author(s): Y. Abe, Y. Aoki, S. Kuriyama, H. Kawame, N. Okamoto, K. Kurosawa, H. Ohashi, S. Mizuno, T. Ogata, S. Kure, T. Niihori, Y. Matsubara.
  • Journal Title: Am. J. Med. Genet. A. Volume: 158A Pages: 1083-1094
  • DOI: 10.1002/ajmg.a.35292
  • Peer Reviewed
• [Journal Article] Two-step biochemical differential diagnosis of classical 21-hydroxylase deficiency and cytochrome P450 oxidoreductase deficiency in Japanese infants using uUrinary Pregnanetriolone / Tetrahydroxycortisone Ratio and 11β-hydroxyandrosterone by Gas chromatography - mass spectrometry. (2012)
  • Author(s): Y. Koyama, K. Homma, M. Fukami, M. Miwa, K. Ikeda, T. Ogata, T. Hasegawa, M. Murata.
  • Journal Title: Clin. Chem. Volume: 58 Pages: 741-747
  • DOI: 10.1373/clinchem.2011.173286
  • Peer Reviewed
• [Journal Article] Deterioration of myocardial tissue Doppler indices in a case of fetal hydrothorax as a promising indication for clinical intervention before the development of nonimmune hydrops fetalis. (2012)
  • Author(s): K. Sekii, H. Itoh, T. Ogata, S. Iwashima.
  • Journal Title: Arch. Gynecol. Obstet. Volume: 286 Pages: 1079-1080
  • DOI: 10.1007/s00404-012-2375-6
  • Peer Reviewed
• [Journal Article] Screening of MAMLD1 mutations in 70 Children with 46,XY DSD: Identification and functional analysis of two new mutations. (2012)
  • Author(s): N. Kalfa, M. Fukami, P. Philibert, F. Audran, C. Pienkowski, G. Weill, C. Pinto, S. Manouvrier, M. Polak, T. Ogata, C. Sultan.
  • Journal Title: PLoS One Volume: 7 Pages: Art. No. e32505
  • DOI: 10.1371/journal.pone.0032505
  • Peer Reviewed
• [Journal Article] Identification of novel low-dose bisphenol A targets in human foreskin fibroblast cells derived from hypospadias patients. (2012)
  • Author(s): X.Y. Qin, M. Miyado, Y. Kojima, H. Zaha, H. Akanuma, Q. Zeng, J. Yoshinaga, J. Yonemoto, M. Fukami, T. Ogata, H. Sone.
  • Journal Title: PLoS One Volume: 7 Pages: Art. No. e36711
  • DOI: 10.1371/journal.pone.0036711
  • Peer Reviewed
• [Journal Article] Fetal myocardial tissue Doppler indices before birth physiologically change in proportion to body size adjusted for gestational age in low-risk term pregnancies. (2012)
  • Author(s): K. Sekii, T. Ishikawa, T. Ogata, H. Itoh, S. Iwashima.
  • Journal Title: Early Hum. Dev. Volume: 88 Pages: 517-523
  • DOI: 10.1016/j.earlhumdev.2011.12.014
  • Peer Reviewed
• [Journal Article] Complex genomic rearrangements in the SOX9 5' region in a patient with Pierre Robin sequence and hypoplastic left scapula. (2012)
  • Author(s): M. Fukami, T. Tsuchiya, S. Takada, A. Kanbara, H. Asahara, A. Igarashi, Y. Kamiyama, G. Nishimura, T. Ogata.
  • Journal Title: Am. J. Med. Genet. A. Volume: 158A Pages: 1529-1534
  • DOI: 10.1002/ajmg.a.35308
  • Peer Reviewed
• [Journal Article] Haplotype analysis of ESR2 in Japanese patients with spermatogenic failure. (2012)
  • Author(s): T. Ogata, M. Fukami, R. Yoshida, E. Nagata, Y. Fujisawa, A. Yoshida, Y. Yoshimura.
  • Journal Title: J. Hum. Genet. Volume: 57 Pages: 449-452
  • DOI: 10.1038/jhg.2012.23
  • Peer Reviewed
• [Journal Article] Association of variants in genes involved in environmental chemical metabolism and risk of cryptorchidism and hypospadias. (2012)
  • Author(s): X.Y. Qin, Y. Kojima, K. Mizuno, K. Ueoka, F. Massart, C. Spinelli, H. Zaha, M. Okura, J. Yoshinaga, J. Yonemoto, K. Kohri, Y. Hayashi, T. Ogata, H. Sone.
  • Journal Title: J. Hum. Genet. Volume: 57 Pages: 434-441
  • DOI: 10.1038/jhg.2012.48
  • Peer Reviewed
• [Journal Article] Characterization of DNA methylation errors in patients with imprinting disorders conceived by assisted reproduction technologies. (2012)
  • Author(s): H. Hiura, H. Okae, N. Miyauchi, F. Sato, A. Sato, M. Van De Pette, R.M. John, M. Kagami, K. Nakai, H. Soejima, T. Ogata, T. Arima.
  • Journal Title: Hum. Reprod. Volume: 27 Pages: 2541-2548
  • DOI: 10.1093/humtep/des197
  • Peer Reviewed
• [Journal Article] PRKAR1A mutation affecting cAMP-mediated G-protein-coupled receptor signaling in a patient with acrodysostosis and hormone resistance. (2012)
  • Author(s): K. Nagasaki, T. Iida, H. Sato, Y. Ogawa, T. Kikuchi, A. Saitoh, T. Ogata, M. Fukami.
  • Journal Title: J. Clin. Endocrinol. Metab. Volume: 97 Pages: E1808-1813
  • DOI: 210/jc.2012-1369
  • Peer Reviewed
• [Journal Article] Paternal uniparental disomy 14 and related disorders: placental gene expression analyses and histological examinations. (2012)
  • Author(s): M. Kagam, K. Matsuoka, T. Nagai, M. Yamanaka, K. Kurosawa, N. Suzumori, Y. Sekita, M. Miyado, K. Matsubara, T. Fuke, F. Kato, M. Fukami, T. Ogata.
  • Journal Title: Epigenetics Volume: 7 Pages: 1142-1150
  • DOI: 10.4161/epi.21937
  • Peer Reviewed
• [Journal Article] Individual variation of the genetic response to low-dose bisphenol A in human foreskin fibroblast cells derived from cryptorchidism and hypospadias patients. (2012)
  • Author(s): X.Y. Qin, H. Sone, Y. Kojima, K. Ueoka, K. Muroya, M. Miyado, H. Zaha, T. Fukuda, J. Yoshinaga, J. Yonemoto, M. Fukami, T. Ogata.
  • Journal Title: PLoS One Volume: 7 Pages: Art. No. e52756
  • DOI: 10.137/journal.pone.0052756
  • Peer Reviewed
• [Journal Article] Identification of INS and KCNJ11 gene mutations in type 1B diabetes in Japanese children with onset of diabetes before 5 yr of age. (2012)
  • Author(s): M. Moritani, I. Yokota, K. Tsubouchi, R. Takaya, K. Takemoto, K. Minamitani, T. Urakami, T. Kawamura, N. Kikuchi, M. Itakura, T. Ogata, S. Sugihara, S. Amemiya.
  • Journal Title: Pediatr. Diabetes Volume: 14 Pages: 112-120
  • DOI: 10.1111/j.1399-5448.2012.00917.x
  • Peer Reviewed
• [Journal Article] Review: Molecular bases and phenotypic determinants of aromatase excess syndrome. (2012)
  • Author(s): M. Fukami, M. Shozu, T. Ogata.
  • Journal Title: Int. J. Endocrinol. Volume: 2012 Pages: Art. No. 584807
  • DOI: 10.1155/2012/584807
  • Peer Reviewed
• [Journal Article] Review: MAMLD1 and 46,XY disorders of sex development. (2012)
  • Author(s): T. Ogata, S. Sano, E. Nagata, F. Kato, M. Fukami.
  • Journal Title: Semi. Reprod. Med. Volume: 30 Pages: 410-416
  • DOI: 10.1055/s-0032-1324725
  • Peer Reviewed
• [Presentation] 腺機能を抑制する新規脳ホルモン、生殖腺刺激ホルモン放出抑制ホルモン（GnIH）の作用機構と発現制御機構. (2012)
  • Author(s): 筒井和義.
  • Organizer: 第35回日本分子生物学会
  • Place of Presentation: 福岡国際会議場(福岡県)
  • Year and Date: 20121213-20121213
  • Invited
• [Presentation] 哺乳類の性分化・性成熟の新知見：性腺機能を制御する遺伝子ネットワークとその破綻による疾患：バックドア経路：テストステロンを経由しないジヒドロテストステロン産生経路. (2012)
  • Author(s): 緒方勤.
  • Organizer: 第35回日本分子生物学会学術集会ワークショップ
  • Place of Presentation: 福岡国際会議場(福岡県)
  • Year and Date: 20121213-20121213
  • Invited
• [Presentation] クサフグの産卵期における下垂体ホルモン遺伝子の発現上昇とLPXRFaペプチドによる調節. (2012)
  • Author(s): 安東宏徳、M. Shahjahan、大杉知裕、浮穴和義、筒井和義、服部淳彦.
  • Organizer: 第37回日本比較内分泌学会大会
  • Place of Presentation: 福井大学（福井県）
  • Year and Date: 20121130-20121130
• [Presentation] 生殖腺刺激ホルモン放出抑制ホルモン(GnIH)は雄ウズラの攻撃行動を抑制する. (2012)
  • Author(s): 産賀崇由、水野貴信、福田裕治郎、筒井和義.
  • Organizer: 第37回日本比較内分泌学会大会
  • Place of Presentation: 福井大学(福井県)
  • Year and Date: 20121130-20121130
• [Presentation] 頭足類マダコの脳におけるニューロステロイド合成. (2012)
  • Author(s): 喜多悠斗、鹿野紀子、原口省吾、南方宏之、筒井和義.
  • Organizer: 第37回日本比較内分泌学会大会
  • Place of Presentation: 福井大学(福井県)
  • Year and Date: 20121130-20121130
• [Presentation] ウズラの松果体は活発にニューロステロイドを合成している. (2012)
  • Author(s): 原口省吾、原桜子、産賀崇由、三田雅敏、筒井和義.
  • Organizer: 第37回日本比較内分泌学会大会
  • Place of Presentation: 福井大学(福井県)
  • Year and Date: 20121130-20121130
• [Presentation] KisslとKiss2の分子進化に関する研究. (2012)
  • Author(s): 大杉知裕、大滝直人、砂川裕也、飯郷雅之、天野勝文、筒井和義.
  • Organizer: 第37回日本比較内分泌学会大会
  • Place of Presentation: 福井大学（福井県）
  • Year and Date: 20121130-20121130
• [Presentation] キンギョの再生ウロコにおける隆起線形成リズム. (2012)
  • Author(s): 黒田美翔、舟橋久幸、鬼木弘明、宇都理佳、筒井和義、鈴木信雄、服部淳彦.
  • Organizer: 第37回日本比較内分泌学会大会
  • Place of Presentation: 福井大学（福井県）
  • Year and Date: 20121130-20121130
• [Presentation] Biosynthesis, mode of action and functional significance of neurosteroids in the Purkinje cell. (2012)
  • Author(s): K. Tsutsui.
  • Organizer: The 15th International Congress on Hormonal Steroids and Hormones & Cancer (ICHSHC)
  • Place of Presentation: Kanazawa (Japan)
  • Year and Date: 20121116-20121116
  • Invited
• [Presentation] Aromatase excess syndrome as a model for genomic disorder: Identification of molecular bases and phenotypic determinants. (2012)
  • Author(s): T. Ogata, M. Shozu, M. Fukami.
  • Organizer: The 7th Asia Pacific Paediatric Endocrine Society Meeting
  • Place of Presentation: Bali (Indonesia)
  • Year and Date: 20121115-20121115
  • Invited
• [Presentation] DNA methylation analysis of reciprocal genome-wide UPDs to define imprinted differentially methylated regions in the human genome. (2012)
  • Author(s): K. Nakabayashi, A.M. Trujillo, C. Tayama, M. Kagami, H. Soejima, T. Ogata, D. Monk, K. Hata.
  • Organizer: American society of human genetics 62nd annual meeting
  • Place of Presentation: Florence (Italy)
  • Year and Date: 20121108-20121108
• [Presentation] 環境ホルモン物質の子どもへの影響. (2012)
  • Author(s): 緒方勤.
  • Organizer: 松葉のダイオキシン調査実行委員会研修会
  • Place of Presentation: 生活クラブ館(東京都)
  • Year and Date: 20121029-20121029
• [Presentation] 成長障害と遺伝子異常. (2012)
  • Author(s): 緒方勤.
  • Organizer: 第11回東海小児内分泌セミナー
  • Place of Presentation: 名古屋市(愛知県)
  • Year and Date: 20121027-20121027
• [Presentation] 全ゲノム片親性ダイソミー症例のＤＮＡメチル化解析によるヒトインプリントーム解明. (2012)
  • Author(s): 中林一彦、田中千春、A.M. Trujillo、岡村浩司、緒方勤、福島英伸、D. Monk、秦 健一郎.
  • Organizer: 第57回日本人類遺伝学会
  • Place of Presentation: 京王プラザホテル(東京都)
  • Year and Date: 20121025-20121025
• [Presentation] Ｈ19-ＤＭＲにメチル化異常を認めたインプリント疾患におけるＨ19-ＤＭＲの変異解析. (2012)
  • Author(s): 東元健、城崎幸介、八木ひとみ、古庄知己、松原圭子、山田大輔、前田寿幸、大塚泰史、古関明彦、緒方勤、副島英伸.
  • Organizer: 第57回日本人類遺伝学会
  • Place of Presentation: 京王プラザホテル(東京都)
  • Year and Date: 20121025-20121025
• [Presentation] シルバーラッセル症候群における新規責任領域の同定. (2012)
  • Author(s): 福家智子、鏡雅代、松原圭子、吉橋博史、長谷川行洋、沼倉周彦、室谷浩二、三善陽子、永井敏郎、長谷川奉延、田中敏章、水野誠司、緒方勤、深見真紀.
  • Organizer: 第57回日本人類遺伝学会
  • Place of Presentation: 京王プラザホテル(東京都)
  • Year and Date: 20121025-20121025
• [Presentation] 14番染色体インプリティング領域メチル化制御機構の解明：ＭＥＧ３-ＤＭＲエピ変異症例の解析から. (2012)
  • Author(s): 鏡雅代、古庄知己、中林一彦、松岡健太郎、松原圭子、福家智子、深見真紀、緒方勤.
  • Organizer: 第57回日本人類遺伝学会
  • Place of Presentation: 京王プラザホテル(東京都)
  • Year and Date: 20121025-20121025
• [Presentation] ホルモン抵抗性を伴うAcrodysostosisの女児における新規PRKAR1A変異の同定と機能解析. (2012)
  • Author(s): 長崎啓祐、深見真紀、緒方勤.
  • Organizer: 第57回日本人類遺伝学会
  • Place of Presentation: 京王プラザホテル(東京都)
  • Year and Date: 20121025-20121025
• [Presentation] 遺伝性女性化乳房（アロマターゼ過剰症）を招くCYP19A1過剰発見にはNHIJ,NAHR,FoSTeSが関与する. (2012)
  • Author(s): 深見真紀、土屋貴義、五十嵐麻希、阿部修司、大津成之、花木啓一、神埼晋、大山建二、佐野友昭、西垣敏紀、高木博史、稲垣朱実、堀川玲子、生水真紀夫、緒方勤.
  • Organizer: 第57回日本人類遺伝学会
  • Place of Presentation: 京王プラザホテル(東京都)
  • Year and Date: 20121025-20121025
• [Presentation] 15番染色体母性方親性ダイソミーを伴うtetrasomy15q症例の分子遺伝学的解析. (2012)
  • Author(s): 松原圭子、柳田かえで、福家智子、鏡雅代、永井敏郎、緒方勤、深見真紀.
  • Organizer: 第57回日本人類遺伝学会
  • Place of Presentation: 京王プラザホテル(東京都)
  • Year and Date: 20121025-20121025
• [Presentation] プラダーウィリ症候群の出生季節性. (2012)
  • Author(s): 綾部匡之、松原圭子、深見真紀、緒方勤、村上信行、永井敏郎.
  • Organizer: 第57回日本人類遺伝学会
  • Place of Presentation: 京王プラザホテル(東京都)
  • Year and Date: 20121025-20121025
• [Presentation] ゴナドトロピン補充療法と生殖補助医療の併用により拳児に至った中枢性性腺機能低下疾患者の一例. (2012)
  • Author(s): 佐藤直子、堀川玲子、勝又規行、内木康博、田中敏章、緒方勤.
  • Organizer: 第57回日本人類遺伝学会
  • Place of Presentation: 京王プラザホテル(東京都)
  • Year and Date: 20121025-20121025
• [Presentation] 次世代遺伝子解析技術を用いた性分化疾患141例の病因解析. (2012)
  • Author(s): 五十嵐麻希、D.C. Vu、小島祥敬、堀川玲子、緒方勤、深見真紀.
  • Organizer: 第57回日本人類遺伝学会
  • Place of Presentation: 京王プラザホテル(東京都)
  • Year and Date: 20121025-20121025
• [Presentation] Novel mechanisms regulating diurnal and seasonal changes in locomotor activity by 7α-hydroxypregnenolone, a new bioactive neurosteroid. (2012)
  • Author(s): K. Tsutsui.
  • Organizer: The 27th International Congress of the International Society for Chronobiology (Focal theme: “Biological Clocks and Health Issues in the 21st Century”
  • Place of Presentation: Delhi (India)
  • Year and Date: 20121005-20121005
  • Invited
• [Presentation] Discovery of gonadotropin-inhibitory hormone (GnIH) and advancement of reproductive neuroendocrinology. (2012)
  • Author(s): K. Tsutsui.
  • Organizer: International Symposium on Frontiers in Biologically Active Peptide. The 3rd Meeting of the Japan Branch of the International Neuropeptide Society (INPS)
  • Place of Presentation: Kitakyushu (Japan)
  • Year and Date: 20120929-20120929
  • Invited
• [Presentation] 男性における乳房腫大(女性化乳房). (2012)
  • Author(s): 深見真紀、五十嵐麻希、阿部修二、山本幸代、金城さおり、緒方勤.
  • Organizer: 第46回日本小児内分泌学会学術集会
  • Place of Presentation: 大阪国際会議場(大阪府)
  • Year and Date: 20120928-20120928
• [Presentation] 入院管理中に書字障害にはじめて気付いた12歳の肥満男児例―行動療法における問題点. (2012)
  • Author(s): 佐竹栄一郎、中川祐一、内山弘基、松下理恵、藤澤泰子、永田絵子、中島信一、緒方勤.
  • Organizer: 第46回日本小児内分泌学会学術集会
  • Place of Presentation: 大阪国際会議場(大阪府)
  • Year and Date: 20120928-20120928
• [Presentation] 次世代解析技術を用いた性分化疾患141症例の病因解析. (2012)
  • Author(s): 五十嵐麻希、D.C. Vu、小島祥敬、堀川玲子、緒方勤、深見真紀.
  • Organizer: 第46回日本小児内分泌学会学術集会
  • Place of Presentation: 大阪国際会議場(大阪府)
  • Year and Date: 20120928-20120928
• [Presentation] NPR2ヘテロ接合性変異は突発性および家族性低身長症の原因となりうる. (2012)
  • Author(s): 天野直子、向井徳男、伊藤義也、鳴海覚志、田中敏章、横谷進、緒方勤、長谷川奉延.
  • Organizer: 第46回日本小児内分泌学会学術集会
  • Place of Presentation: 大阪国際会議場(大阪府)
  • Year and Date: 20120928-20120928
• [Presentation] シルバーラッセル症候群の新規責任領域の同定. (2012)
  • Author(s): 福家智子、松原圭子、鏡雅代、長谷川行洋、永井敏郎、長谷川奉延、水野誠司、緒方勤、深見真紀.
  • Organizer: 第46回日本小児内分泌学会学術集会
  • Place of Presentation: 大阪国際会議場(大阪府)
  • Year and Date: 20120928-20120928
• [Presentation] 14染色体母親性ダイソミーおよび類縁疾患に関する全国調査：遺伝学的頻度および臨床像. (2012)
  • Author(s): 鏡雅代、長崎啓祐、佐藤英利、鹿島京子、佐藤亨、中村明枝、加藤光弘、沼倉周彦、緒方勤、深見真紀、斎藤伸治.
  • Organizer: 第46回日本小児内分泌学会学術集会
  • Place of Presentation: 大阪国際会議場(大阪府)
  • Year and Date: 20120928-20120928
• [Presentation] 偽性副甲状腺機能低下症la型と類似の臨床像を呈する女児に同定した新規PRKAR14変異例. (2012)
  • Author(s): 長崎啓祐、佐藤英利、小川洋平、菊池透、緒方勤、深見真紀.
  • Organizer: 第46回日本小児内分泌学会学術集会
  • Place of Presentation: 大阪国際会議場(大阪府)
  • Year and Date: 20120928-20120928
• [Presentation] プラダーウィリ症候群の出生季節性. (2012)
  • Author(s): 綾部匡之、松原圭子、深見真紀、緒方勤、村上信行、永井敏郎.
  • Organizer: 第46回日本小児内分泌学会学術集会
  • Place of Presentation: 大阪国際会議場(大阪府)
  • Year and Date: 20120928-20120928
• [Presentation] 小児インスリン治療研究会遺伝素因プロジェクトメンバー1型糖尿病の出生季節性：発症時年齢とＶＤＲ遺伝子多型に関する検討. (2012)
  • Author(s): 綾部匡之、深見真紀、緒方勤、雨宮伸、杉原茂孝.
  • Organizer: 第46回日本小児内分泌学会学術集会
  • Place of Presentation: 大阪国際会議場(大阪府)
  • Year and Date: 20120928-20120928
• [Presentation] ＭＡＭＬＤ1と尿道下裂：新規遺伝子変異および感受性ハプロタイプの同定と変異陽性患者の長期精巣機能. (2012)
  • Author(s): 佐野伸一朗、深見真紀、宮戸真美、上松あゆ美、長谷川奉延、佐竹栄一郎、C. Sultan、緒方勤.
  • Organizer: 第46回日本小児内分泌学会学術集会
  • Place of Presentation: 大阪国際会議場(大阪府)
  • Year and Date: 20120928-20120928
• [Presentation] Nicotinamid Nucleotide Transhydrogenase遺伝子の新規変異が同定された糖質コルチコイド欠乏症症例. (2012)
  • Author(s): 山口理恵、永田絵子、藤澤泰子、長崎啓祐、緒方勤.
  • Organizer: 第46回日本小児内分泌学会学術集会
  • Place of Presentation: 大阪国際会議場(大阪府)
  • Year and Date: 20120928-20120928
• [Presentation] 雌性生殖器官におけるＭａｍｌｄ１の役割. (2012)
  • Author(s): 宮戸真美、宮戸健二、緒方勤、深見真紀.
  • Organizer: 第46回日本小児内分泌学会学術集会
  • Place of Presentation: 大阪国際会議場(大阪府)
  • Year and Date: 20120928-20120928
• [Presentation] Ambiguous genitaliaを呈するSRY(+)46,XXDSD症例における分子細胞遺伝学的解析. (2012)
  • Author(s): 中島信一、大石彰、加藤芙弥子、中西俊樹、五十嵐麻希、深見真紀、緒方勤.
  • Organizer: 第46回日本小児内分泌学会学術集会
  • Place of Presentation: 大阪国際会議場(大阪府)
  • Year and Date: 20120928-20120928
• [Presentation] ＩＭＡＧｅ症候群におけるＣＤＫＮ１Ｃ遺伝解析. (2012)
  • Author(s): 加藤芙弥子、天野直子、永田絵子、中西俊樹、堀川玲子、澤田浩武、福家智子、深見真紀、長谷川奉延、緒方勤.
  • Organizer: 第46回日本小児内分泌学会学術集会
  • Place of Presentation: 大阪国際会議場(大阪府)
  • Year and Date: 20120928-20120928
• [Presentation] 46, XY性分化疾患患者における 2 番染色体長腕部分欠失の同定. (2012)
  • Author(s): 鈴木江莉奈、五十嵐麻希、宮戸真美、D.C. Vu、緒方勤、深見真紀.
  • Organizer: 第46回日本小児内分泌学会学術集会
  • Place of Presentation: 大阪国際会議場(大阪府)
  • Year and Date: 20120928-20120928
• [Presentation] ゴナドトロピン補充療法と生殖補助医療の併用により拳児に至った中枢性性腺機能低下疾患者の一例. (2012)
  • Author(s): 佐藤直子、堀川玲子、内木康弘、田中敏章、勝又規行、緒方勤.
  • Organizer: 第46回日本小児内分泌学会学術集会
  • Place of Presentation: 大阪国際会議場(大阪府)
  • Year and Date: 20120928-20120928
• [Presentation] 早期にＧＨ治療を開始した重度発達遅滞を伴うターナー症候群の１例. (2012)
  • Author(s): 小川玲、藤本陽子、児玉雅彦、布山正貴、塚田大樹、松橋一彦、外山大輔、池田裕一、緒方勤、磯山恵一.
  • Organizer: 第46回日本小児内分泌学会学術集会
  • Place of Presentation: 大阪国際会議場(大阪府)
  • Year and Date: 20120928-20120928
• [Presentation] 広汎性発達障害に思春期早発症を合併した女児の4例. (2012)
  • Author(s): 中西俊樹、藤澤泰子、佐竹栄一郎、鈴木輝彦、佐野伸一朗、松下理恵、永田絵子、山口理恵、中島信一、緒方勤.
  • Organizer: 第46回日本小児内分泌学会学術集会
  • Place of Presentation: 大阪国際会議場(大阪府)
  • Year and Date: 20120928-20120928
• [Presentation] 経時的にコルチゾール分泌能が低下したＰＯＲ異常症の一例. (2012)
  • Author(s): 沼倉周彦、北中幸子、本間桂子、深見真紀、長谷川奉延、緒方勤、早坂清.
  • Organizer: 第46回日本小児内分泌学会学術集会
  • Place of Presentation: 大阪国際会議場(大阪府)
  • Year and Date: 20120928-20120928
• [Presentation] 大後頭孔狭窄をきたし軟骨無形成症様症状を呈した複合型下垂体前葉機能低下症の１例. (2012)
  • Author(s): 遠藤彰、杉江秀夫、緒方勤.
  • Organizer: 第46回日本小児内分泌学会学術集会
  • Place of Presentation: 大阪国際会議場(大阪府)
  • Year and Date: 20120928-20120928
• [Presentation] Catch upのないIUGRモデルラットにおける脂肪量とアディポサイトカインの変化について. (2012)
  • Author(s): 橘田一輝、中西俊樹、永田絵子、山口理恵、中川祐一、大関武彦、石井正浩、緒方勤.
  • Organizer: 第46回日本小児内分泌学会学術集会
  • Place of Presentation: 大阪国際会議場(大阪府)
  • Year and Date: 20120928-20120928
• [Presentation] 生殖腺刺激ホルモン放出抑制ホルモンGnIH：発見、研究の進展と展望. (2012)
  • Author(s): 筒井和義.
  • Organizer: 第81回日本動物学会大会シンポジウム：比較生理生化学の醍醐味－ICCPB2011国際比較生理生化学会に向けて－
  • Place of Presentation: 東京大学 (東京都)
  • Year and Date: 20120924-20120924
  • Invited
• [Presentation] 活動量を高める7α-ヒドロキシプレグネノロンはサケの遡上行動を誘導する. (2012)
  • Author(s): 原口省吾、張雋螢、山本雄三、小山鉄平、鈴木悠子、上田宏、筒井和義.
  • Organizer: 第83回日本動物学会大会
  • Place of Presentation: 大阪大学 (大阪府)
  • Year and Date: 20120915-20120915
• [Presentation] 鳴声刺激は短日条件下にあるウズラの生殖活動を高める. (2012)
  • Author(s): 産賀崇由、長谷川喜久、中野真樹、丸山雄介、服部淳彦、筒井和義.
  • Organizer: 第83回日本動物学会大会
  • Place of Presentation: 大阪大学 (大阪府)
  • Year and Date: 20120915-20120915
• [Presentation] メスの存在がオスウズラの視床下部－下垂体－生殖腺系に及ぼす影響. (2012)
  • Author(s): 戸張靖子、長谷川喜久、筒井和義.
  • Organizer: 第83回日本動物学会大会
  • Place of Presentation: 大阪大学 (大阪府)
  • Year and Date: 20120915-20120915
• [Presentation] GnIH はAC/cAMP/PKA/ERK 系を抑制してゴナドトロピン分泌を抑制する. (2012)
  • Author(s): ソンユリ、産賀崇由、筒井和義.
  • Organizer: 第83回日本動物学会大会
  • Place of Presentation: 大阪大学 (大阪府)
  • Year and Date: 20120915-20120915
• [Presentation] 雄イモリ腹腺由来ステロイドに対する雌鋤鼻嗅覚上皮の応答性. (2012)
  • Author(s): 豊田ふみよ、中田友明、原口省吾、山本和俊、筒井和義、菊山榮.
  • Organizer: 第83回日本動物学会大会
  • Place of Presentation: 大阪大学 (大阪府)
  • Year and Date: 20120915-20120915
• [Presentation] ヒガシナメクジウオの新規RFa ペプチドの同定. (2012)
  • Author(s): 岡村友貴、大杉知裕、逸見泰久、筒井和義.
  • Organizer: 第83回日本動物学会大会
  • Place of Presentation: 大阪大学 (大阪府)
  • Year and Date: 20120915-20120915
• [Presentation] ヤツメウナギの脳におけるGnIH オーソログの同定と機能解析. (2012)
  • Author(s): 大杉知裕、ダークスダナ、ガズダクリステン、産賀崇由、コスギタカヨシ、野崎眞澄、サワーステイシア、筒井和義.
  • Organizer: 第83回日本動物学会大会
  • Place of Presentation: 大阪大学 (大阪府)
  • Year and Date: 20120915-20120915
• [Presentation] Evolutionary origin of gonadotropin-inhibitory hormone: Insights from lampreys. (2012)
  • Author(s): K. Tsutsui.
  • Organizer: The 7th International Symposium on Fish Endocrinology
  • Place of Presentation: Buenos Aires (Argentina)
  • Year and Date: 20120903-20120903
  • Invited
• [Presentation] Characterization of novel RFamide peptides in the central nervous system of the brown hagfish. (2012)
  • Author(s): T. Osugi, K. Uchida, M. Nozaki, K. Tsutsui.
  • Organizer: The 7th International Symposium on Fish Endocrinology
  • Place of Presentation: Buenos Aires (Argentina)
  • Year and Date: 20120903-20120903
• [Presentation] ヌーナン症候群とからだの成長. (2012)
  • Author(s): 緒方勤.
  • Organizer: 厚生労働科研費ヌーナン症候群シンポジウム
  • Place of Presentation: 東京国際フォーラム(東京都)
  • Year and Date: 20120818-20120818
• [Presentation] ゲノム異常としての性分化疾患. (2012)
  • Author(s): 緒方勤.
  • Organizer: 第11回熊本内分泌代謝フォーラム
  • Place of Presentation: 熊本市(熊本県)
  • Year and Date: 20120817-20120817
• [Presentation] 小児の成長と成長障害. (2012)
  • Author(s): 緒方勤.
  • Organizer: 浜松市健康増進課研修会
  • Place of Presentation: 浜松市(静岡県)
  • Year and Date: 20120725-20120725
• [Presentation] 生殖を制御する新規脳ホルモンの作用機構と発現制御機構. (2012)
  • Author(s): 筒井和義.
  • Organizer: ファイザー講演会「New Insights of molecular Genetics on Growth Disorders」
  • Place of Presentation: 富士ソフトアキバプラザ (東京都)
  • Year and Date: 20120714-20120714
  • Invited
• [Presentation] Discovery, progress and prospect of gonadotropin-inhibitory hormone (GnIH), a new key regulator of reproduction. (2012)
  • Author(s): K. Tsutsui.
  • Organizer: The 5th BRC-UK International Conference Symposium on Neuroscience
  • Place of Presentation: Jeju (Korea)
  • Year and Date: 20120628-20120628
  • Invited
• [Presentation] Gonadotropin-inhibitory hormone (GnIH) in the zebra finch: Peptide isolation, cDNA cloning and brain distribution. (2012)
  • Author(s): Y. Tobari, N. Iijima, K. Tsunekawa, T. Osugi, H. Ozawa, K. Tsutsui.
  • Organizer: The 10th International Symposium on Avian Endocrinology (ISAE2012)
  • Place of Presentation: Gifu (Japan)
  • Year and Date: 20120609-20120609
• [Presentation] Gonadotropin-inhibitory hormone precursor mRNA is induced during depressed food intake in the heat-exposed chick brain. (2012)
  • Author(s): V.S. Chowdhury, S. Tomonaga, S. Nishimura, S. Tabata, J.F. Cockrem, T. Osugi, T. Ubuka, K. Tsutsui, M. Furuse.
  • Organizer: The 10th International Symposium on Avian Endocrinology (ISAE2012)
  • Place of Presentation: Gifu (Japan)
  • Year and Date: 20120609-20120609
  • Invited
• [Presentation] Identification, localization and function of a novel neuropeptide, 26RFa, and its cognate receptor, GPR103 in the avian hypothalamus. (2012)
  • Author(s): K. Ukena, T. Tachibana, J. Leprince, H. Vaudry, K. Tsutsui.
  • Organizer: The 10th International Symposium on Avian Endocrinology (ISAE2012)
  • Place of Presentation: Gifu (Japan)
  • Year and Date: 20120609-20120609
  • Invited
• [Presentation] インプリンティング疾患研究アップデート. (2012)
  • Author(s): 緒方勤.
  • Organizer: 第45回新潟小児内分泌懇話会
  • Place of Presentation: 新潟市(新潟県)
  • Year and Date: 20120608-20120608
• [Presentation] Melatonin regulates diurnal changes in locomotor activity by regulating 7α-hydroxypregnenolone synthesis in quail. (2012)
  • Author(s): S. Haraguchi, K. Inoue, H. Miyabara, S. Suzuki, Y. Ogura, K. Tsutsui.
  • Organizer: The 10th International Symposium on Avian Endocrinology (ISAE2012)
  • Place of Presentation: Gifu (Japan)
  • Year and Date: 20120607-20120607
  • Invited
• [Presentation] Discovery of gonadotropin-inhibitory hormone (GnIH) and advancement of avian neuroendocrinology. (2012)
  • Author(s): K. Tsutsui, G.E. Bentley, T. Ubuka, V.S. Chowdhury, G. Bedecarrats, P.J. Sharp, J.C. Wingfield.
  • Organizer: The 10th International Symposium on Avian Endocrinology (ISAE2012)
  • Place of Presentation: Gifu (Japan)
  • Year and Date: 20120605-20120605
  • Invited
• [Presentation] Gonadotropin-inhibitory hormone (GnIH) receptor and cell signaling. (2012)
  • Author(s): T. Ubuka, Y.L. Son, G.E. Bentley, R.P. Millar, K. Tsutsui.
  • Organizer: The 10th International Symposium on Avian Endocrinology (ISAE2012)
  • Place of Presentation: Gifu (Japan)
  • Year and Date: 20120605-20120605
  • Invited
• [Presentation] Effects of thyroid disrupting pesticides on hypothalamo-pituitary-testicular axis of a tropical bird, Amandava amandava. (2012)
  • Author(s): B. Mohanty, S.P. Pandey, S. Aamir, K. Tsutsui.
  • Organizer: The 10th International Symposium on Avian Endocrinology (ISAE2012)
  • Place of Presentation: Gifu (Japan)
  • Year and Date: 20120605-20120605
• [Presentation] Cytochrome P450 oxidoreductase deficiency: A unique disease leading to disorders of sex development in both 46,XY and 46,XX patients. (2012)
  • Author(s): T. Ogata.
  • Organizer: The 6th International Symposium on the Biology of Vertebrate Sex Determination
  • Place of Presentation: Kona (USA)
  • Year and Date: 20120425-20120425
  • Invited
• [Presentation] 性腺機能を調節するGnIHの作用機構と発現制御機構. (2012)
  • Author(s): 筒井和義.
  • Organizer: 第84回日本内分泌学会学術総会
  • Place of Presentation: 神戸国際展示場(兵庫県)
  • Year and Date: 20120421-20120421
  • Invited
• [Presentation] 胎児期における環境化学物質と先天奇形の発症. (2012)
  • Author(s): 緒方勤.
  • Organizer: 第115回日本小児科学会学術集会：子どもの健康と環境に関する全国調査（エコチル調査）と小児内分泌代謝疾患
  • Place of Presentation: 福岡国際会議場(福岡県)
  • Year and Date: 20120421-20120421
• [Presentation] Neuroendocrine regulation of neurosteroid biosynthesis by hypothalamic neurohormones. (2012)
  • Author(s): H. Vaudry, J.Y. Seong, D. Burel, G. Pelletier, M.C. Tonon, K. Tsutsui, J.L. Do Rego.
  • Organizer: Korean Endocrine Society
  • Place of Presentation: Seoul (Korea)
  • Year and Date: 20120420-20120420
  • Invited
• [Presentation] 性分化疾患とゲノム異常. (2012)
  • Author(s): 緒方勤.
  • Organizer: 第15回宮崎代謝内分泌研究会
  • Place of Presentation: 宮崎市(宮崎県)
  • Year and Date: 20120411-20120411
• [Book] The E-Book of Neurosteroids: Frontiers Research Topic (2013)
  • Author(s): H. Vaudry, K. Tsutsui.
  • Total Pages: 476
  • Publisher: Frontiers in Endocrinology
• [Book] Neurosteroids and synaptic formation in the cerebellum. In: Handbook of Cerebellum and Cerebellar Disorders (2013)
  • Author(s): K. Tsutsui.
  • Total Pages: 1277中10
  • Publisher: Springer
• [Book] Gonadotropin-inhibitory hormone (GnIH). In: The Handbook of Biologically Active Peptides (2nd edition) (2013)
  • Author(s): K. Tsutsui, T. Ubuka.
  • Total Pages: 1640中10
  • Publisher: Elsevier publisher
• [Book] 26RFa. In: The Handbook of Biologically Active Peptides (2nd edition) (2013)
  • Author(s): J. Leprince, C. Neveu, B. Lefrance, L. Guilhaudis, I. Segalas-Milazzo, J.C. Do Rego, M. Tena-Sempere, K. Tsutsui, H. Vaudry.
  • Total Pages: 1640中8
  • Publisher: Elsevier publisher
• [Book] 高等学校「生物」 (2013)
  • Author(s): 筒井和義.
  • Total Pages: 432
  • Publisher: 第一学習社
• [Book] 高等学校「生物基礎」 (2013)
  • Author(s): 筒井和義.
  • Total Pages: 304
  • Publisher: 第一学習社
• [Book] 高等学校「新生物基礎」 (2013)
  • Author(s): 筒井和義.
  • Total Pages: 176
  • Publisher: 第一学習社