生殖制御における新規脳内分子機構の解明

2014 Fiscal Year Annual Research Report

• Project/Area Number: 22227002
• Research Institution: Waseda University
• Principal Investigator: 筒井 和義 早稲田大学, 教育・総合科学学術院, 教授 (20163842)
• Co-Investigator(Kenkyū-buntansha): 緒方 勤 浜松医科大学, 医学部, 教授 (40169173)
• Project Period (FY): 2010-04-01 – 2015-03-31
• Keywords: 視床下部ホルモン / 脳下垂体ホルモン / 発現制御 / シグナル伝達 / 生殖 / 生殖行動 / 生殖機能異常 / 分子進化

Outline of Annual Research Achievements

「GnIH生殖抑制作用の分子機構」：GnIHニューロンが投射するキスペプチンニューロンにはGnIH受容体が発現していることを明らかにした。次に、GnIHはキスペプチンニューロンに作用してキスペプチンニューロンの活動を制御することを見いだした。「GnIH発現の分子制御機構」：GnIHニューロンにはメラトニン、グルココルチコイド、活性型甲状腺ホルモンの受容体が発現していることを明らかにした。次に、GnIHニューロンにおけるこれらのホルモンによるGnIH発現の分子制御機構を明らかにした。「生殖腺におけるGnIHの局所作用機構」： GnIHの局所作用により卵巣と精巣のステロイド合成酵素の発現と活性が低下してステロイド合成が抑制されることを明らかにした。「GnIHによる生殖行動の発現制御機構」：GnIHは脳内に発現するP450aromの脱リン酸化を導き、P450arom活性とエストロゲン合成を増加させて生殖行動の発現を制御することを明らかにした。「GnIH作用の生理的意義」：GnIH遺伝子のノックアウトマウスを作製してGnIH作用の生理的意義を解析した。その結果、GnIHは生殖腺の機能と生殖行動の発現を抑制することを明らかにした。さらに、GnIHの低下により春期早発が導かれることが示唆された。「GnIHによるヒト生殖機能異常の分子機構」：集積した生殖機能異常症患者のDNA検体を用い、次世代シーケンサーによりGnIH遺伝子とGnIH受容体遺伝子の変異解析と機能解析を行った。「動物界におけるGnIHとGnIH受容体の起源と分子進化」：原索動物（ナメクジウオ）からGnIHとGnIH受容体の祖先型遺伝子を同定した。霊長類から無顎類に至る脊椎動物と原索動物から同定したGnIH遺伝子とGnIH受容体遺伝子のシンテニー解析を行い、GnIHとGnIH受容体の起源と分子進化を明らかにした。

Research Progress Status

26年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

26年度が最終年度であるため、記入しない。

Research Products

• [Journal Article] GnIH and GnRH expressions in the central nervous system and pituitary of Indian major carp, Labeo rohita during ontogeny: An immunocytochemical study. (2015)
  • Author(s): S. Biswas, A. G. Jadhao, C. Pinelli, N. V. Palande and K. Tsutsui
  • Journal Title: Gen. Comp. Endocrinol. Volume: 印刷中 Pages: 印刷中
  • DOI: 10.1016/j.ygcen.2014.06.005
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Duration of melatonin regulates seasonal neural plasticity in subtropical Indian weaver bird, Ploceus philippinus. (2015)
  • Author(s): S. Thakur, Y. Kumari, S. Rani, K. Tsutsui and V. Kumar
  • Journal Title: Gen. Comp. Endocrinol. Volume: 印刷中 Pages: 印刷中
  • DOI: 10.1016/j.ygcen.2014.06.004
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Neuroanatomical organization of brain gonadotropin-inhibitory hormone and gonadotropin-releasing hormone systems in the frog Pelophylax esculentus. (2015)
  • Author(s): C. Pinelli, A. G. Jadhao, S. P. Biswas, K. Tsutsui and B. D’Aniello
  • Journal Title: Brain Behav. Evol. Volume: 印刷中 Pages: 印刷中
  • DOI: 10.1159/000368594
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] A unique mechanism of successful fertilization in a domestic bird. (2015)
  • Author(s): T. Sasanami, S. Izumi, N. Sakurai, T. Hirata, S. Mizushima, M. Matsuzaki, G. Hiyama, E. Yorinaga, T. Yoshimura, K. Ukena and K. Tsutsui
  • Journal Title: Sci. Rep. Volume: 5 Pages: 7700
  • DOI: 10.1038/srep07700
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Review: Seasonal control of gonadotropin-inhibitory hormone (GnIH) in birds and mammals. (2015)
  • Author(s): L. J. Kriegsfeld, T. Ubuka, G. E. Bentley and K. Tsutsui
  • Journal Title: Front. Neuroendocrinol. Volume: 印刷中 Pages: 印刷中
  • DOI: 10.1016/j.yfrne.2014.12.001
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Review: An evolutionary scenario for GnIH in chordates. (2015)
  • Author(s): T. Osugi, T. Ubuka and K. Tsutsui
  • Journal Title: J. Neuroendocrinol. Volume: 印刷中 Pages: 印刷中
  • DOI: 10.1111/jne.12246
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] SOX3 overdosage permits normal sex development in females with random X inactivation. (2015)
  • Author(s): M. Igarashi, H. Mikami, M. Katsumi, M. Miyado, Y. Izumi, T. Ogata and M. Fukami
  • Journal Title: Sex. Dev. Volume: 印刷中 Pages: 印刷中
  • DOI: 10.1159/000377653
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Novel splice site mutation in MAMLD1 in a patient with hypospadias. (2015)
  • Author(s): M. Igarashi, Y. Wada, Y. Kojima, M. Miyado, M. Nakamura, K. Muroya, K. Mizuno, Y. Hayashi, K. Nonomura, K. Jofri, T. Ogata and M. Fukami
  • Journal Title: Sex. Dev. Volume: 印刷中 Pages: 印刷中
  • DOI: 10.1159/000380842
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Comprehensive clinical studies in 34 patients with molecularly defined UPD(14)pat and related conditions (Kagami-Ogata syndrome). (2015)
  • Author(s): M. Kagami, K. Kurosawa, O. Miyazaki, F. Ishino, K. Matsuoka and T. Ogata
  • Journal Title: Eur. J. Hum. Genet. Volume: 印刷中 Pages: 印刷中
  • DOI: 10.1038/ejhg.2015.13
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Hypogonadotropic hypogonadism in a female patient previously diagnosed as having Waardenburg syndrome due to a SOX10 mutation. (2015)
  • Author(s): Y. Izumi, I. Musha, E. Suzuki, M. Iso, T. Jinno, R. Horikawa, S. Amemiya, T. Ogata, M. Fukami and A. Ohtake
  • Journal Title: Endocrine. Volume: 印刷中 Pages: 印刷中
  • DOI: 10.1007/s12020-014-0434-4
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Epimutations of the IG-DMR and the MEG3-DMR at the 14q32.2 imprinted region in two patients with Silver-Russell syndrome-compatible phenotype. (2015)
  • Author(s): M. Kagami, S. Mizuno, K. Matsubars, K. Nakabayashi, S. Sano, T. Fuke, M. Fukami and T. Ogata
  • Journal Title: Eur. J. Hum. Genet. Volume: 印刷中 Pages: 印刷中
  • DOI: 10.1038/ejhg.2014.234
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Copy-number variations in Y chromosomal azoospermia factor regions identified by multiplex ligation-dependent probe amplification. (2015)
  • Author(s): K. Saito, M. Miyado, Y. Kobori, Y. Tanaka, H. Ishikawa, A. Yoshida, M. Katsumi, H. Saito, T. Kubota, H. Okada, T. Ogata and M. Fukami
  • Journal Title: J. Hum. Genet. Volume: 60 Pages: 127-131
  • DOI: 10.1038/jhg.2014.115
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Silver-Russell syndrome without body asymmetry in three patients with duplications of maternally derived chromosome 11p15 involving CDKN1C. (2015)
  • Author(s): S. Nakashima, F. Kato, T. Kosho, K. Nagasaki, T. Kikuchi, M. Kagami, M. Fukami and T. Ogata
  • Journal Title: J. Hum. Genet. Volume: 60 Pages: 91-95
  • DOI: 10.1038/jhg.2014.100
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] A 45,X/46,XY DSD (Disorder of Sexual Development) case with an extremely uneven distribution of 46,XY cells between lymphocytes and gonads. (2015)
  • Author(s): R. Nomura, K. Miyai, M. Okada, M. Kajiwara, M. Ono, T. Ogata, I. Onishi, M. Sato, M. Sekine, T. Akashi, S. Mizutani and K. Kashimada
  • Journal Title: Clin. Pediatr. Endocrinol. Volume: 24 Pages: 11-14
  • DOI: 10.1297/cpe.24.11
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Low temperature-induced circulating triiodothyronine accelerates seasonal testicular regression. (2014)
  • Author(s): K. Ikegami, Y. Atsumi, E. Yorinaga, H. Ono, I. Murayama1, Y. Nakane, W. Ota, N. Arai, A. Tega, M. Iigo, V. M. Darras, K. Tsutsui, Y. Hayashi, S. Yoshida and T. Yoshimura
  • Journal Title: Endocrinology Volume: 56 Pages: 647-659
  • DOI: 10.1210/en.2014-1741
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Inhibitory roles of the mammalian GnIH ortholog RFRP-3 in testicular activities in adult mice. (2014)
  • Author(s): S. Anjum, A. Krishna and K. Tsutsui
  • Journal Title: J. Endocrinol. Volume: 223 Pages: 79-91
  • DOI: 10.1530/JOE-14-0333
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Gonadotropin-inhibitory hormone-stimulation of food intake is mediated by hypothalamic effects in chicks. (2014)
  • Author(s): B. McConn, G. Wang, J. Yi, E. Gilbert, T. Osugi, T. Ubuka, K. Tsutsui, V. Chowdhury, M. Furuse and M. Cline
  • Journal Title: Neuropeptides Volume: 48 Pages: 327-334
  • DOI: 10.1159/000368594
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] A new pathway mediating social effects on the endocrine system: female presence acting via norepinephrine release stimulates gonadotropin-inhibitory hormone in the paraventricular nucleus and suppresses luteinizing hormone in quail. (2014)
  • Author(s): Y. Tobari, Y. L. Son, T. Ubuka, Y. Hasegawa and K. Tsutsui
  • Journal Title: J. Neurosci. Volume: 34 Pages: 9803-9811
  • DOI: 10.1523/JNEUROSCI.3706-13.2014
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Involvement of Gas-proteins in the action of relaxin-like gonad-stimulating substance on starfish ovarian follicle cells. (2014)
  • Author(s): M. Mita, S. Haraguchi, M. Watanabe, Y. Takeshige, K. Yamamoto and K. Tsutsui
  • Journal Title: Gen. Comp. Endocrinol. Volume: 205 Pages: 80-87
  • DOI: 10.1016/j.ygcen.2014.06.003
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Evolutionary origin of GnIH and NPFF in chordates: Insights from novel amphioxus RFamide peptides. (2014)
  • Author(s): T. Osugi, T. Okamura, Y. L. Son, M. Ohkubo, T. Ubuka, Y. Henmi and K. Tsutsui
  • Journal Title: PLoS ONE Volume: 9 Pages: e100962
  • DOI: 10.1371/journal.pone.0100962
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Differential expressions of RFamide-related peptide, a mammalian gonadotropin-inhibitory hormone ortholog, and kisspeptin in the hypothalamus of Abadeh ecotype does during breeding and anestrous seasons. (2014)
  • Author(s): M. R. J. Shirazi, M. J. Zamiri, M. S. Salehi, S. Moradi, A. Tamadon, M. R. Namavar, A. Akhlaghi, K. Tsutsui and A. Caraty
  • Journal Title: J. Neuroendocrinol. Volume: 26 Pages: 186-194
  • DOI: 10.1111/jne.12137
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Molecular basis for the activation of gonadotropin-inhibitory hormone gene transcription by corticosterone. (2014)
  • Author(s): Y. L. Son, T. Ubuka, M. Narihiro, Y. Fukuda, I. Hasunuma, K. Yamamoto, D. D. Belsham and K. Tsutsui
  • Journal Title: Endocrinology Volume: 155 Pages: 1817-1826
  • DOI: 10.1210/en.2013-2076
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Hypothalamic inhibition of socio-sexual behaviour by increasing neuroestrogen synthesis. (2014)
  • Author(s): T. Ubuka, S. Haraguchi, Y. Tobari, M. Narihiro, K. Ishikawa, T. Hayashi, N. Harada and K. Tsutsui
  • Journal Title: Nat. Commun. Volume: 5 Pages: 3061
  • DOI: 10.1038/ncomms4061
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Review: Evolution of GnIH and related peptides structure and function in the chordates. (2014)
  • Author(s): T. Osugi, T. Ubuka and K. Tsutsui
  • Journal Title: Front. Neurosci. Volume: 8 Pages: 255
  • DOI: 10.3389/fnins.2014.00255
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Review: Evolution of gonadotropin-inhibitory hormone receptor and its ligand. (2014)
  • Author(s): T. Ubuka and K. Tsutsui
  • Journal Title: Gen. Comp. Endocrinol. Volume: 209 Pages: 148-161
  • DOI: 10.1016/j.ygcen.2014.09.002
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Review: Breakthrough in neuroendocrinology by discovering novel neuropeptides and neurosteroids: 1) Discovery of gonadotropin-inhibitory hormone (GnIH) across vertebrates. (2014)
  • Author(s): K. Tsutsui and T. Ubuka
  • Journal Title: Gen. Comp. Endocrinol. Volume: 205 Pages: 4-10
  • DOI: 10.1016/j.ygcen.2014.03.006
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Review: Breakthrough in neuroendocrinology by discovering novel neuropeptides and neurosteroids: 2) Discovery of neurosteroids and pineal neurosteroids. (2014)
  • Author(s): K. Tsutsui and S. Haraguchi
  • Journal Title: Gen. Comp. Endocrinol. Volume: 205 Pages: 11-22
  • DOI: 10.1016/j.ygcen.2014.03.008
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Review: Gonadotropin-inhibitory hormone inhibits aggressive behavior of male quail by increasing neuroestrogen synthesis in the brain beyond its optimum concentration. (2014)
  • Author(s): T. Ubuka and K. Tsutsui
  • Journal Title: Gen. Comp. Endocrinol. Volume: 205 Pages: 49-54
  • DOI: 10.1016/j.ygcen.2014.03.014
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Review: Biosynthesis and biological action of pineal allopregnanolone. (2014)
  • Author(s): K. Tsutsui and S. Haraguchi
  • Journal Title: Front. Cell. Neurosci. Volume: 8 Pages: 118
  • DOI: 10.3389/fncel.2014.00118
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Review: Molecular evolution and function of 26RFa/QRFP and its cognate receptor. (2014)
  • Author(s): K. Ukena, T. Osugi, J. Leprince, H. Vaudry and K. Tsutsui
  • Journal Title: J. Mol. Endocrinol. Volume: 52 Pages: T119-T131
  • DOI: 10.1530/JME-13-0207
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Review: Central and direct regulation of testicular activity by gonadotropin-inhibitory hormone and its receptor. (2014)
  • Author(s): T. Ubuka, Y. L. Son, Y. Tobari, M. Narihiro, G. E. Bentley, L. Kriegsfeld and K. Tsutsui
  • Journal Title: Front. Endocrinol. Volume: 5 Pages: 8
  • DOI: 10.3389/fendo.2014.00008
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Japanese founder duplications/triplications involving BHLHA9 are associated with split-hand/foot malformation with or without long bone deficiency and Gallop-Wolfgang complex. (2014)
  • Author(s): E. Nagata, H. Kano, F. Kato, R. Yamaguchi, S. Nakashima, S. Takayams, R. Kosaki, H. Tonoki, S. Mizuno, S. Watanabe, K. Yoshiura, T. Kosho, T. Hasegawa, M. Kimizuka, A. Suzuki, K. Shimizu, H. Ohashi, N. Haga, H. Numabe, E. Horii, T. Nagai, H. Yoshihashi, M. Fukami, S. Ikegawa and T. Ogata
  • Journal Title: Orphanet J. Rare Dis. Volume: 9 Pages: 125
  • DOI: 10.1186/s13023-014-0125-5
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Clinical and molecular studies in four patients with SRY-positive 46,XX testicular disorders of sex development: implications for variable sex development and genomic rearrangements. (2014)
  • Author(s): S. Nakashima, A. Oishi, F. Takada, H. Kawamura, M. Igarashi, M. Fukami and T. Ogata
  • Journal Title: J. Hum. Genet. Volume: 59 Pages: 549-553
  • DOI: 10.1038/jhg.2014.70
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Rationale and study design of the Japan environment and children’s study (JECS). (2014)
  • Author(s): T. Kawamoto, H. Nitta, K. Murata, E. Toda, N. Tsukamoto, M. Hasegawa, Z. Yamagata, F. Kayama, R. Kshi, Y. Ohya, H. Saito, H. Sago, M. Okuyama, T. Ogata, S. Yokoya, Y. Koresawa, Y. Shibata, S. Nakayama, T. Michikawa, A. Takeuchi and H. Saitoh
  • Journal Title: BMC. Public Health Volume: 14 Pages: 25
  • DOI: 10.1186/1471-2458-14-25
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Genome-wide copy number analysis and systematic mutation screening in 58 patients with hypogonadotropic hypogonadism. (2014)
  • Author(s): Y. Izumi, E. Suzuki, S. Kanzaki, S. Yatsuga, S. Kinjo, M. Igarashi, T. Maruyama, S. Sano, R. Horikawa, N. Sato, K. Nakabayashi, K. Hata, A. Umezawa, T. Ogata, Y. Yoshimura and M. Fukami
  • Journal Title: Fertil. Steril. Volume: 102 Pages: 1130-1136
  • DOI: 10.1016/j.fertnstert.2014.06.017
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Comprehensive and quantitative multilocus methylation analysis reveals the susceptibility of specific imprinted differentially methylated regions (DMRs) to aberrant methylation in Beckwith-Wiedemann syndrome with epimutations. (2014)
  • Author(s): T. Maeda, K. Higashimoto, K. Jozaki, H. Hitomi, K. Nakabayashi, Y. Makita, H. Tonoki, N. Okamoto, F. Takada, H. Ohashi, M. Migita, R. Kosaki, K. Matsubara, T. Ogata, M. Matsuo, Y. Hamasaki, Y. Ohtsuka, K. Nishioka, K. Joh, T. Mukai, K. Hata and H. Soejima
  • Journal Title: Genet. Med. Volume: 16 Pages: 903-912
  • DOI: 10.1038/gim.2014.46
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] A novel de novo point mutation of OCT-binding site in the IGF2/H19-imprinting control region in a patient with Beckwith-Wiedemann syndrome. (2014)
  • Author(s): K. Higashimoto, K. Jozaki, T. Kosho, K. Matsubara, T. Sato, D. Yamada, H. Yatsuki, T. Maeda, Y. Ohtsuka, K. Nishioka, K. Joh, H. Koseki, T. Ogata and H. Soejima
  • Journal Title: Clin. Genet. Volume: 86 Pages: 539-544
  • DOI: 10.1111/cge.12318
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] De novo frameshift mutation in fibroblast growth factor 8 in a male patient with gonadotropin deficiency. (2014)
  • Author(s): E. Suzuki, S. Yatsuga, M. Igarashi, M. Miyado, K. Nakabayashi, K. Hayashi, K. Hata, A. Umezawa, G. Yamada, T. Ogata and M. Fukami
  • Journal Title: Horm. Res. Paediatr. Volume: 81 Pages: 139-144
  • DOI: 10.1159/000355380
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Early vitamin K deficiency bleeding in a neonate associated with maternal Crohn's disease. (2014)
  • Author(s): A. Ohishi, S. Nakashima, T. Ogata and S. Iijima
  • Journal Title: J. Perinatol. Volume: 34 Pages: 636-639
  • DOI: 10.1038/jp.2014.64
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Uniparental disomy of chromosome 8 leading to homozygosity of a CYP11B1 mutation in a patient with congenital adrenal hyperplasia: Implication for a rare etiology of an autosomal recessive disorder. (2014)
  • Author(s): K. Matsubara, N. Kataoka, S. Ogita, S. Sano, T. Ogata, M. Fukami and N. Katsumata
  • Journal Title: Endocr. J. Volume: 61 Pages: 629-633
  • DOI: 10.1507/endocrj.EJ13-0509
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] A missense single-nucleotide polymorphism in the sialic acid acetylesterase (SIAE) gene is associated with anti-PIT-1 antibody syndrome. (2014)
  • Author(s): M. Yamamoto, G. Iguchi, H. Bando, H. Fukuoka, K. Suda, M. Takahashi, H. Nishizawa, R. Matsumoto, K. Tojo, A. Mokubo, T. Ogata and Y. Takahashi
  • Journal Title: Endocr. J. Volume: 61 Pages: 641-644
  • DOI: 10.1507/endocrj.EJ13-0539
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] TBX1 mutation identified by exome sequencing in a Japanese family with 22q11.2 deletion syndrome-like craniofacial features and hypocalcemia. (2014)
  • Author(s): T. Ogata, T. Niihori, N. Tanaka, M. Kawai, T. Nagashima, R. Funayama, K. Nakayama, S. Nakashim, F. Kato, M. Fukami, Y. Aoki and Y. Matsubara
  • Journal Title: PLoS ONE Volume: 9 Pages: e91598
  • DOI: 10.1371/journal.pone.0091598
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Identification and functional characterization of two novel NPR2 mutations in Japanese patients with short stature. (2014)
  • Author(s): N. Amano, T. Mukai, Y. Ito, S. Narumi, T. Tanaka, S. Yokoya, T. Ogata and T. Hasegawa
  • Journal Title: J. Clin. Endocrinol. Metab. Volume: 99 Pages: E13-E18
  • DOI: 10.1210/jc.2013-3525
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Genome-wide parent-of-origin DNA methylation analysis reveals the intricacies of the human imprintome and suggests a germline methylation independent establishment of imprinting. (2014)
  • Author(s): F. Court, C. Tayama, V. Romanelli, A. Martin-Trujillo, I. Iglesias-Platas, K. Okamura, N. Sugahara, C. Simon, H. Moore, J. Harness, H. Keirstead, J. Vicente Sanchez-Mut, E. Kaneki, P. Lapunzina, H. Soejima, N. Wake, M. Esteller, T. Ogata, K. Hata, K. Nakabayashi and D. Monk
  • Journal Title: Genome Res. Volume: 24 Pages: 554-569
  • DOI: 10.1101/gr.164913.113
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Aromatase excess syndrome in a family with upstream deletion of CYP19A1. (2014)
  • Author(s): D. Shihara, M. Miyado, K. Nakabayashi, M. Shozu, K. Nagasaki, T. Ogata and M. Fukami
  • Journal Title: Clin. Endocrinol. Volume: 81 Pages: 314-316
  • DOI: 10.1111/cen.12329
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Lack of genomic rearrangements involving the aromatase gene CYP19A1 in breast cancer. (2014)
  • Author(s): M. Fukami, J. Suzuki, K. Nakabayashi, R. Tsunashima, T. Ogata, M. Shozu and S. Noguchi
  • Journal Title: Breast Cancer Volume: 21 Pages: 382-385
  • DOI: 10.1007/s12282-013-0471-5
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Review: Cytochrome P450 oxidoreductase deficiency: Rare congenital disorder leading to skeletal malformations and steroidogenic defects. (2014)
  • Author(s): M. Fukami and T. Ogata
  • Journal Title: Pediatr. Int. Volume: 56 Pages: 805-808
  • DOI: 10.1111/ped.12518
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Review: Understanding the pathological manifestations of aromatase excess syndrome: lessons for the clinic. (2014)
  • Author(s): M. Shozu, M. Fukami and T. Ogata
  • Journal Title: Expert Rev. Endocrinol. Metab. Volume: 9 Pages: 397-409
  • DOI: 10.1586/17446651.2014.926810
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] 生殖制御の新規脳内分子機構 (2015)
  • Author(s): 筒井和義
  • Organizer: 日本生殖再生医学会・第10回学術集会
  • Place of Presentation: メルパルク京都、京都市
  • Year and Date: 2015-03-22 – 2015-03-22
  • Invited
• [Presentation] ノルエピネフリンが雌へのホルモン応答を引き起こす (2014)
  • Author(s): 戸張靖子、ソンユリ、産賀崇由、長谷川喜久、筒井和義
  • Organizer: 第25回マイクロダイアリシス研究会
  • Place of Presentation: お茶の水女子大学、東京都
  • Year and Date: 2014-12-20 – 2014-12-20
• [Presentation] 雌の存在が雄ウズラの生殖内分泌システムを変化させる (2014)
  • Author(s): 戸張靖子、ソンユリ、産賀崇由、長谷川喜久、筒井和義
  • Organizer: 第38回鳥類内分泌研究会
  • Place of Presentation: 紀州鉄道熱海ホテル、熱海市
  • Year and Date: 2014-12-13 – 2014-12-13
• [Presentation] Acute stress increases the synthesis of 7alpha-hydroxypregnenolone, a new key neurosteroid regulating locomotor activity, through corticosterone action in newts. (2014)
  • Author(s): S. Haraguchi, H. Vaudry and K. Tsutsui
  • Organizer: The 8th International Symposium on Amphibian and Reptilian Endocrinology and Neurobiology
  • Place of Presentation: Okazaki, Japan
  • Year and Date: 2014-11-07 – 2014-11-09
  • Invited
• [Presentation] Involvement of multiple hormones in the expression of courtship behavior by the male newt, Cynops pyrrhogaster (2014)
  • Author(s): F. Toyoda, I. Hasunuma, T. Nakada, S. Haraguchi, K. Tsutsui and S. Kikuyama
  • Organizer: The 8th International Symposium on Amphibian and Reptilian Endocrinology and Neurobiology
  • Place of Presentation: Okazaki, Japan
  • Year and Date: 2014-11-07 – 2014-11-09
• [Presentation] Analysis of cDNA sequences of relaxin-like gonad-stimulating substance in starfish Asterina pectinifera from Japanese waters (2014)
  • Author(s): N. Ikeda, S. Haraguchi, K. Tsutsui, M. Mita
  • Organizer: 第39回日本比較内分泌学会大会
  • Place of Presentation: 岡崎コンファレンスセンター、岡崎市
  • Year and Date: 2014-11-07 – 2014-11-09
• [Presentation] Mammalian GnIH inhibits aggressive behavior of male mouse (2014)
  • Author(s): S. Okada, M. Narihiro, T. Ubuka and K. Tsutsui
  • Organizer: 第39回日本比較内分泌学会大会
  • Place of Presentation: 岡崎コンファレンスセンター、岡崎市
  • Year and Date: 2014-11-07 – 2014-11-09
• [Presentation] Developmental changes in the expressions of gonadotropin-inhibitory hormone and its receptor in the Japanese quail (Coturnix japonica) brain (2014)
  • Author(s): K. Ishikawa, T. Ubuka and K. Tsutsui
  • Organizer: 第39回日本比較内分泌学会大会
  • Place of Presentation: 岡崎コンファレンスセンター、岡崎市
  • Year and Date: 2014-11-07 – 2014-11-09
• [Presentation] Gonadotropin-inhibitory hormone (GnIH) inhibits socio-sexual behaviors of male quail by increasing neuroestrogen synthesis in the brain (2014)
  • Author(s): T. Ubuka, S. Haraguchi, Y. Tobari, M. Narihiro, K. Ishikawa and K. Tsutsui
  • Organizer: 第39回日本比較内分泌学会大会
  • Place of Presentation: 岡崎コンファレンスセンター、岡崎市
  • Year and Date: 2014-11-07 – 2014-11-09
• [Presentation] ニューロステロイド研究の進展：ニューロンの生存と発達を制御するニューロステロイドの生合成と作用機構 (2014)
  • Author(s): 筒井和義
  • Organizer: 第22回ステロイドホルモン学会学術集会
  • Place of Presentation: 都道府県会館、東京都
  • Year and Date: 2014-11-03 – 2014-11-03
  • Invited
• [Presentation] メラトニンとニューロステロイドによる自発運動の日周リズム制御 (2014)
  • Author(s): 原口省吾、筒井和義
  • Organizer: 第41回日本神経内分泌学会学術集会
  • Place of Presentation: 都道府県会館、東京都
  • Year and Date: 2014-11-01 – 2014-11-01
  • Invited
• [Presentation] 松果体で合成されたアロプレグナノロンは小脳のPACAP発現を制御する (2014)
  • Author(s): 原口省吾、時田巧磨、田代渓一郎、松田恒平、筒井和義
  • Organizer: 第85回大会日本動物学会
  • Place of Presentation: 東北大学川内北キャンパス、仙台市
  • Year and Date: 2014-09-11 – 2014-09-13
• [Presentation] マウスの松果体におけるニューロステロイド合成酵素の発現 (2014)
  • Author(s): 時田巧磨、原口省吾、筒井和義
  • Organizer: 第85回大会日本動物学会
  • Place of Presentation: 東北大学川内北キャンパス、仙台市
  • Year and Date: 2014-09-11 – 2014-09-13
• [Presentation] ゼブラフィッシュの松果体におけるニューロステロイド合成酵素の発現 (2014)
  • Author(s): 田代渓一郎、原口省吾、筒井和義
  • Organizer: 第85回大会日本動物学会
  • Place of Presentation: 東北大学川内北キャンパス、仙台市
  • Year and Date: 2014-09-11 – 2014-09-13
• [Presentation] ヒトデ生殖腺刺激ホルモンGSSの作用機構に対するGsタンパク質の影響 (2014)
  • Author(s): 三田雅敏、原口省吾、山本和俊、筒井和義
  • Organizer: 第85回大会日本動物学会
  • Place of Presentation: 東北大学川内北キャンパス、仙台市
  • Year and Date: 2014-09-11 – 2014-09-13
• [Presentation] 生殖生理を変化させる社会的刺激受容の神経内分泌機構 (2014)
  • Author(s): 戸張靖子、ソンユリ、産賀崇由、長谷川喜久、筒井和義
  • Organizer: 第85回大会日本動物学会
  • Place of Presentation: 東北大学川内北キャンパス、仙台市
  • Year and Date: 2014-09-11 – 2014-09-13
• [Presentation] マウス脳内においてGnIHがGPR147を介してGnRHおよびアロマターゼを制御する可能性 (2014)
  • Author(s): 成廣美里、産賀崇由、筒井和義
  • Organizer: 第85回大会日本動物学会
  • Place of Presentation: 東北大学川内北キャンパス、仙台市
  • Year and Date: 2014-09-11 – 2014-09-13
• [Presentation] Integrated neuroendocrine control of newt locomotor behavior. (2014)
  • Author(s): S. Haraguchi, H. Vaudry and K. Tsutsui
  • Organizer: The 27th Conference of European Comparative Endocrinologists
  • Place of Presentation: Rennes, France
  • Year and Date: 2014-08-25 – 2014-08-29
  • Invited
• [Presentation] CRH stimulates neurosteroid biosynthesis in the frog brain: Possible implication in the control of the stress response. (2014)
  • Author(s): Jean Luc Do Rego, K. Tsutsui and H. Vaudry
  • Organizer: The 27th Conference of European Comparative Endocrinologists
  • Place of Presentation: Rennes, France
  • Year and Date: 2014-08-25 – 2014-08-29
  • Invited
• [Presentation] Biosynthesis and biological action of pineal allopregnanolone in birds. (2014)
  • Author(s): K. Tsutsui
  • Organizer: The 26th International Ornithological Congress
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2014-08-18 – 2014-08-24
  • Invited
• [Presentation] Gonadotropin-inhibitory hormone inhibits socio-sexual behaviors by increasing neuroestrogen synthesis in the male quail. (2014)
  • Author(s): T. Ubuka, S. Haraguchi, Y. Tobari, M. Narihiro, K. Ishikawa, T. Hayashi, N. Harada and K. Tsutsui
  • Organizer: The 26th International Ornithological Congress
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2014-08-18 – 2014-08-24
• [Presentation] Evolution of GnIH structures and functions in chordates. (2014)
  • Author(s): K. Tsutsui
  • Organizer: The 8th International Congress of Neuroendocrinology
  • Place of Presentation: Sydney, Australia
  • Year and Date: 2014-08-17 – 2014-08-20
  • Invited
• [Presentation] Environmental impacts on male sexual differentiation. (2014)
  • Author(s): T. Ogata
  • Organizer: The 16th International Congress of Endocrinology & the Endocrine Society’s 96th Annual Meeting & Expo
  • Place of Presentation: Chicago, USA
  • Year and Date: 2014-06-21 – 2014-06-24
  • Invited
• [Book] Gonadotropin-inhibitory hormone actions in the brain and pituitary. In: The E-Book: Frontiers Research Topic on Neuropeptide GPCRs in Neuroendocrinology (2014)
  • Author(s): T. Ubuka, Y. L. Son, Y. Tobari and K. Tsutsui
  • Total Pages: pp. 211-224
  • Publisher: Frontiers in Endocrinology