Regulatory Basis of Biomotility: Chemo-mechanical Feedback Loop

2015 Fiscal Year Final Research Report

• Project/Area Number: 22227005
• Research Category: Grant-in-Aid for Scientific Research (S)
• Allocation Type: Single-year Grants
• Research Field: Biophysics
• Research Institution: Waseda University
• Principal Investigator: Ishiwata Shin'ichi 早稲田大学, 理工学術院, 教授 (10130866)
• Co-Investigator(Renkei-kenkyūsha): TAKEOKA Shinji 早稲田大学, 理工学術院, 教授 (20222094) ; SUZUKI Madoka 早稲田大学, 重点領域研究機構, 主任研究員(准教授) (40350475) ; MIYAZAKI Makito 早稲田大学, 理工学術院, 助教 (40609236) ; ITABASHI Takeshi 早稲田大学, 理工学術院, 助教 (20434384) ; Sergey V. Mikhailenko 早稲田大学, 理工学術院, 准教授 (10424805) ; SHIMOYAMA Isao 東京大学, 大学院情報理工学系研究科, 教授 (60154332) ; FUKUDA Norio 東京慈恵会医科大学, 細胞生理学教室, 准教授 (30301534) ; SHIMAMOTO Yuta 国立遺伝学研究所, 准教授 (80409656) ; OGUCHI Yusuke 東京大学, 理学系研究科・生物科学専攻・生物化学科, 特任助教 (40599370) ; OYAMA Kotaro 東京慈恵会医科大学, 細胞生理学教室, 学振PD (70632131) ; SHINTANI Seine 東京大学, 理学系研究科・物理学専攻, 学振PD (40650536) ; TAKAGI Jun 国立遺伝学研究所, 博士研究員 (40632196) ; SATO Katsuhiko 北海道大学, 電子科学研究所, 准教授 (90513622)
• Project Period (FY): 2010-04-01 – 2016-03-31
• Keywords: 生体分子モーター / １分子生物学 / 化学力学フィードバック / 筋収縮自励振動 / SPOC / 細胞分裂 / 人工細胞 / 細胞熱力学

Outline of Final Research Achievements

The main purpose of this study is to make clear the existence of Chemo-mechanical feedback loop (CMF loop) in the regulation of elementary processes of bio-motility such as intracellular transport, oscillation and cell division. We have demonstrated several examples of CMF loop such as 1) self-oscillation (SPOC) phenomena in the contractile system of muscle, i.e., "force generation due to myosin cross-bridges (CB), sliding motion of myofilaments, change in the lattice spacing of myofilaments, change in the probability of CB formation", 2) the timing of chromosome segregation within a HeLa cell depends on the direction of externally applied mechanical impulse, and 3) the realization of the spontaneous formation of contractile ring within a cell-sized confined space, showing the importance of boundary condition for the ordered cytoskeletal structure.

Free Research Field

生物物理学