Post-transcriptional regulation associated with RNA modifications responsible for higher order biological processes

2014 Fiscal Year Final Research Report

• Project/Area Number: 22227006
• Research Category: Grant-in-Aid for Scientific Research (S)
• Allocation Type: Single-year Grants
• Research Field: Molecular biology
• Research Institution: The University of Tokyo
• Principal Investigator: SUZUKI Tsutomu 東京大学, 工学(系)研究科(研究院), 教授 (20292782)
• Project Period (FY): 2010-05-31 – 2015-03-31
• Keywords: RNA修飾 / RNAエディティング / mRNA / tRNA

Outline of Final Research Achievements

This project aims to study regulatory gene expression associated with various biological processes mediated by post-transcriptional modifications. We established a biochemical method called "inosine chemical erasing (ICE)" to directly identify inosines on RNA strands with high reliability, and applied the ICE method combined with deep sequencing (ICE-seq) to successfully map about 20,000 novel A-to-I RNA editing sites in human brain transcriptome. As novel RNA modifications, we identified 2-agmatinylcytidine (agm2C) and cyclic t6A (ct6A) from tRNAs, and studies their function and biogenesis.

Free Research Field

RNA生化学