2012 Fiscal Year Final Research Report
Development of diagnostic tools for personalized cancer medicine by genomic and epigenomic analyses
| Project/Area Number |
22240090
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Tumor diagnosis
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
INAZAWA Johji 東京医科歯科大学, 難治疾患研究所, 教授 (30193551)
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| Co-Investigator(Kenkyū-buntansha) |
KOZAKI Kenichi 東京医科歯科大学, 難治疾患研究所, 准教授 (50270715)
INOUE Jun 東京医科歯科大学, 難治疾患研究所, 助教 (50568326)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 難治がん / 癌遺伝子 / 癌抑制遺伝子 / ゲノム / エピゲノム / マイクロRNA / DNAメチル化 / 分子標的薬 |
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| Research Abstract |
In order to develop useful diagnostic tools for personalized cancer medicine, we performed genetic and epigenomic analyses in cancer, and identified novel cancer-related genes. By using methylation-based screening of tumor suppressor (TS) -miRNAs in oral squamous cell carcinoma (OSCC), we identified miR-596 and it target gene, LGALS3BP. Epithelial-mesenchymal transition (EMT) is relevant to the mechanism of invasion and metastasis in cancer. From a comprehensive gene expression analysis using colorectal cancer (CRC) cell lines, and following the ontology (GO) analysis, SIX1 was identified as an EMT-related gene in CRC. Furthermore, through DNA methylation and gene expression analyses of components in the Wnt signaling pathway, we identified WNT7A and WNT10A as genes silenced by mesenchymal-specific DNA hypermethylation in oral OSCC. In autophagy LC3 has a physiologically important role. Among LC3 family genes, we identified two transcriptional variants of LC3A, LC3Av1 and LC3Av2. Interestingly, we found that LC3Av1 functions in autophagy and indicated that LC3Av1 may be crucial in various types of cancer.
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[Journal Article] Common variants at 11q12, 10q26 and 3p11.2 are associated with prostate cancer susceptibility in Japanese2012
Author(s)
Akamatsu S, Takata R, Haiman CA, Takahashi A, Inoue T, Kubo M, Furihata M, Kamatani N, Inazawa J, Chen GK, Le Marchand L, Kolonel LN, Katoh T,Yamano Y, Yamakado M, Takahashi H, Yamada H, Egawa S, Fujioka T, Henderson BE, Habuchi T, Ogawa O, Nakamura Y, Nakagawa H
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Journal Title
Nat Genet
Volume: 44
Pages: 426-9
DOI
Peer Reviewed
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[Journal Article] Variation in TP63 is associated with lung adenocarcinoma susceptibility inJapanese and Korean populations2010
Author(s)
Miki D, Kubo M, Takahashi A, Yoon KA, Kim J, Lee GK, Zo JI, Lee JS, Hosono N, Morizono T, Tsunoda T, Kamatani N, Chayama K, Takahashi T, Inazawa J, Nakamura Y, Daigo Y
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Journal Title
NatGenet
Volume: 42
Pages: 893-6
DOI
Peer Reviewed
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[Journal Article] Genome-wide association study identifies five new susceptibility loci for prostate cancer in the Japanese population2010
Author(s)
Takata R, Akamatsu S, Kubo M, Takahashi A, Hosono N, Kawaguchi T, Tsunoda T, Inazawa J, Kamatani N,Ogawa O, Fujioka T, Nakamura Y, Nakagawa H
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Journal Title
Nat Genet
Volume: 42
Pages: 751-4
DOI
Peer Reviewed
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[Journal Article] Frequent silencing of protocadherin 17, a candidate tumour suppressor for esophageal squamous-cell carcinoma2010
Author(s)
Haruki S, Imoto I, Kozaki K, Matsui T, Kawachi H, Komatsu S, Muramatsu T, Shimada Y, Kawano T, Inazawa J
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Journal Title
Carcinogenesis
Volume: 31
Pages: 1027-36
DOI
Peer Reviewed
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