Expression-pattern based studies on gene regulation network in the nemetode C.elegans

2012 Fiscal Year Final Research Report

• Project/Area Number: 22241047
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Genome biology
• Research Institution: National Institute of Genetics
• Principal Investigator: KOHARA Yuji 国立遺伝学研究所, 系統生物研究センター, 特任教員 (70135292)
• Project Period (FY): 2010 – 2012
• Keywords: 遺伝子 / 発現調節 / ゲノム / 発生分化 / mRNA / 3'-UTR / 局在化

Research Abstract

We have found the cis- and trans-elements for the localization of pos-1 mRNA to the posterior side and mex-3 mRNA to the anterior side in the 2 cell stage embryo of C.elegans, and have obtained the clue to understand the mechanisms of mRNA localization. 2) Based on the analysis of β-catenin HMP-2 in C.elegans early embryos, we have proposed that HMP-2 functioned downstream of the Src signaling pathway and contributed to endoderm induction and ABar spindle orientation, in parallel with the Wnt signaling pathway. 3) We identified the regions of promoter activity for gcy-8 and gcy-18 , essential genes for the thermosensory neuron AFD, and found that the transcription factors CEH-11 and TTX-1 functioned in the regulation of the gene expression coordinately

Research Products

• [Journal Article] Convey and H. Niki: Multi-Decadal Survival of an Antarctic Nematode, Plectus Murrayi, in a -20°C Stored Moss Sample. (2012)
  • Author(s): H. Kagoshima, K. Kito, T. Aizu, T. Shin-i, H. Kanda, S. Kobayashi, A. Toyoda, A. Fujiyama,Y. Kohara.P
  • Journal Title: CryoLetters Volume: Volume 33, No 4
  • Peer Reviewed
• [Journal Article] Kampkotter A, Bossinger O.: C. elegans VANG-1 Modulates Life Span via Insulin/IGF-1-Like Signaling (2012)
  • Author(s): Honnen SJ, Buchter C, Schroder V, Hoffmann M, Kohara Y
  • Journal Title: PLoS One
  • Peer Reviewed
• [Journal Article] The beta-catenin HMP-2 functions downstream of Src in parallel with the Wnt pathway in early embryogenesis of C. elegans (2011)
  • Author(s): E. Sumiyoshi, S. Takahashi, H. Obata, A. Sugimoto and Y. Kohara
  • Journal Title: Developmental Biology Volume: 355 Pages: 302-312
  • Peer Reviewed
• [Journal Article] K. C. Gunsalus, J. K. Kim: The Landscape of C. elegans 3'UTRs (2010)
  • Author(s): M. Mangone, A. P. Manoharan, D. Thierry-Mieg, J. Thierry-Mieg, T. Han, S. Mackowiak, E. Mis, C. Zegar, M. R. Gutwein, V. Khivansara, O. Attie, K. Chen, K. Salehi-Ashtiani, M. Vidal, T. T. Harkins, P. Bouffard, Y. Suzuki, S. Sugano, Y. Kohara, N. Rajewsky, F. Piano,
  • Journal Title: Science Volume: 329 Pages: 432-435
  • DOI: 10.1126/science.1191244
  • Peer Reviewed
• [Presentation] The homeobox transcription factors, CEH-14 and TTX-1, regulate AFD thermosensory neuron-specific expression of gcy-8 and gcy-18 in C.elegans (2012)
  • Author(s): 鹿児島浩、小原雄治
  • Organizer: 日本分子生物学会第35回年会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-12
• [Presentation] In situ hybridization analysis of let-7 microRNA. 18th International C.elegans Meeting (2011)
  • Author(s): Y. Andachi, Y. Kohara
  • Organizer: UCLA, Los Angeles
  • Place of Presentation: USA
  • Year and Date: 2011-06-25
• [Presentation] The homeobox transcription factors, CEH-14 and TTX-1, regulate expression of gcy-8 and gcy-18 in C. elegans (2011)
  • Author(s): H. Kagoshima, Y. Kohara
  • Organizer: 18th International C.elegans Meeting
  • Place of Presentation: UCLA, Los Angeles, USA
  • Year and Date: 2011-06-24
• [Presentation] Study on localization mechanisms of maternal mex-3 mRNA. (2011)
  • Author(s): Konno, H., Noguchi, K., & Kohara, Y
  • Organizer: 18th International C.elegans Meeting
  • Place of Presentation: UCLA, Los Angeles, USA
  • Year and Date: 2011-06-23
• [Presentation] Studies on localization mechanisms of the maternal pos-1 mRNA in C (2010)
  • Author(s): K. Noguchi, Y. Kohara
  • Organizer: 第33回日本分子生物学会年
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-08
• [Presentation] 線虫C. elegansの温度感受神経AFDでの発現を制御する調節配列の解析 (2010)
  • Author(s): 鹿児島浩、小原雄治
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-08
• [Remarks] NEXTDB:線虫遺伝子発現データベース
  • URL: http://nematode.lab.nig.ac.jp/