Biomedical studies of insect models for human diseases to understand their pathogenic mechanism and development of a novel screening method to identify candidates of medicines for human diseases using the insect disease models

2012 Fiscal Year Final Research Report

• Project/Area Number: 22241052
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Living organism molecular science
• Research Institution: Kyoto Institute of Technology
• Principal Investigator: MORI Hajime 京都工芸繊維大学, 工芸科学研究科, 教授 (80201812)
• Co-Investigator(Kenkyū-buntansha): YAMAGUCHI Masamitsu 京都工芸繊維大学, 工芸科学研究科, 教授 (00182460) ; TAKETANI Shigeru 京都工芸繊維大学, 工芸科学研究科, 教授 (20121949) ; KAMEI Kaeko 京都工芸繊維大学, 工芸科学研究科, 教授 (00214544) ; KOTANI Eiji 京都工芸繊維大学, 工芸科学研究科, 准教授 (10273541) ; YOSHIDA Hideki 京都工芸繊維大学, 工芸科学研究科, 助教 (30570600) ; INOUE Yoshihiro 京都工芸繊維大学, 昆虫バイオメディカル教育研究センター, 准教授 (90201938)
• Project Period (FY): 2010 – 2012
• Keywords: 昆虫 / 生体機能利用 / 遺伝学 / 蛋白質 / 脳神経疾患

Research Abstract

A neuron-specific depletion of Drosophila orthologue of human responsible gene for ALS resulted in ALS-like phenotypes in Drosophila. It is possible to speculate that ALS may be caused by a reduction of the gene products rather than accumulation of abnormal aggregates. A developmental study of Drosophila diabetes model suggests that activation of the JNK cascade by several types of stress in insulin-producing cells resulted in diabetes-like phenotypes. We constructed polyhedra immobilized BMP-2 growth factor in polyhedral microcrystals from silkworm virus and showed that the implanted polyhedra have activity of bone regeneration on rat calvarial bone.

Research Products

• [Journal Article] Orbit/CLASP is required for germline cyst formation through its developmental control of fusomes and ring canals in Drosophila males (2013)
  • Author(s): Miyauchi, C., Kitazawa, D., Ando, I. and Inoue, Y. H
  • Journal Title: An essential gene for male germline cyst formation Volume: 8(3)
  • DOI: DOI:10.1371/journal.pone.0058220
  • Peer Reviewed
• [Journal Article] Bone regeneration by polyhedral microcrystals from silkworm virus. (2012)
  • Author(s): Matsumoto, G., Ueda, T., Shimoyama, J., Ijiri, H., Omi, Y., Yube, H., Sugita, Y., Kubo, K., Maeda, H., Kinoshita, Y., Arias, D. G.G., Shimabukuro, J., Kotani, E., Kawamata, S., and Mori, H.
  • Journal Title: Sci. Rep. Volume: 2巻 Pages: 935
  • DOI: DOI:10.1038/srep00935
  • Peer Reviewed
• [Journal Article] Fibroblast growth factor-2 (Fgf2) is an important factor that maintains cellular immaturity and contributes to aggressiveness of osteosarcoma (2012)
  • Author(s): Shimizu, T., Ishikawa, T., Iwai, S., Ueki, A., Sugihara, E., Onishi, N., Kuninaka, S., Miyamoto, T., Toyama, Y., Ijiri, H., Mori, H., Matsuzaki, Y., Yaguchi, T., Nishio, H., Kawakami, Y., Ikeda, Y., Saya, H.
  • Journal Title: Mol. Cancer Res Volume: 10巻 Pages: 454-468
  • DOI: DOI:10.1158/1541-7786.MCR-11-0347
  • Peer Reviewed
• [Journal Article] XNP/dATRX interacts with DREF in the chromatin to regulate gene expression. (2012)
  • Author(s): Valadez-Graham, V., Yoshioka, Y., Velazquez, O., Kawamori, A. Vazquez.,M., Neumann, A., Yamaguchi, M. and Zurita, M
  • Journal Title: Nucleic Acids Res Volume: 1巻 Pages: 1460-1474
  • DOI: DOI:10.1093/nar/gkr865
  • Peer Reviewed
• [Journal Article] 、Polycomb repressive complex 2-dependent and ?independent functions of Jarid2 in transcriptional regulation in Drosophila (2012)
  • Author(s): Herz, H.-M., Mohan, M., Garrett, A.-S., Miller, C., Casto, D., Zhang, Y., Seidel, C., Haug, J. S., Florens, L., Washburn, M. P., Yamaguchi, M.Shiekhattar, R. and Shilatifard, A.
  • Journal Title: Mol. Cell. Biol Volume: 132 (9)巻 Pages: 1683-1693
  • DOI: DOI:10.1128/MCB.06503-11
  • Peer Reviewed
• [Journal Article] MLF is a conserved regulator of RUNX transcription factor activity involved in hematopoiesis (2012)
  • Author(s): Bras, S., Martin-Lanneree, S., Gobert, V., Auge, B., Breig, O., Sanial, M., Yamaguchi, M., Haenlin, M., Plessis, A. and Waltzer, L
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 1109 (13)巻 Pages: 4986-4991
  • DOI: DOI:10.1073/pnas.1117317109
  • Peer Reviewed
• [Journal Article] Depletion of glutamine enhances sodium butyrate-induced erythroid differentiation of K562 cells.Depletion of glutamine enhances sodium butyrate-induced erythroid differentiation of K562 cells. (2012)
  • Author(s): Hiep,N. C., Kinohira, S., Furuyama, K and Taketani S
  • Journal Title: J. Biochem Volume: 152(6) Pages: 509-519
  • DOI: DOI:10.1093/jb/mvs097
  • Peer Reviewed
• [Journal Article] COPI-mediated membrane trafficking is required for cytokinesis in Drosophila male meiotic divisions (2012)
  • Author(s): Kitazawa, D., Yamaguchi, M., Mori, H. Inoue, Y. H
  • Journal Title: J. Cell Sci Volume: 125巻 Pages: 3649-3660
  • DOI: DOI:10.1242/?jcs.103317
  • Peer Reviewed
• [Journal Article] A Drosophila model for the screening of bioavailable NADPH oxidase inhibitors and antioxidants (2011)
  • Author(s): Tu Anh, N. T., Nishitani, M., Harada, S., Yamaguchi, M and Kamei, K
  • Journal Title: Mol. Cell. Biochem Volume: 352(1-2)巻 Pages: 91-8
  • DOI: DOI:10.1007/s11010-011-0743-3
  • Peer Reviewed
• [Journal Article] Syntrophin-2 is required for eye development in Drosophila. Exp (2010)
  • Author(s): Nagai, R., Hashimoto, R., Tanaka, Y., Taguchi, O., Sato, M., Matsukage, A and Yamaguchi, M.
  • Journal Title: Cell. Res Volume: 316 Pages: 272-285
  • DOI: DOI:10.1016/j.yexcr.2009.10.009
  • Peer Reviewed
• [Presentation] 鉄シャペロンfrataxin発現のp53による調節とミトコンドリアのヘム代謝の関係 (2012)
  • Author(s): 竹谷 茂
  • Organizer: 第85回日本生化学会
  • Place of Presentation: 福岡市
  • Year and Date: 20121214-15
• [Presentation] Cabezaノックダウンはショウジョウバエ成虫複眼の形態異常を誘導する (2012)
  • Author(s): 島村 真衣、東 裕美子、笹山 博司、徳田 隆 彦、中川 正法、吉田 英樹、山口 政光
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡市
  • Year and Date: 20121211-14
• [Presentation] Polo and KLP3A-Feo complex are required for accumulation of Orbit/CLASP on contractile ring in Drosophila male meiosis. (2012)
  • Author(s): Kitazawa, D., Miyauchi, C. and Inoue, Y. H.
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡市
  • Year and Date: 20121211-14
• [Presentation] カイコ後部絹糸腺における細胞増殖因子固定化多角体の発現 (2012)
  • Author(s): 山本尚人・小谷英治・山本賢二・内野恵郎・瀬筒秀 樹・田村俊樹・森 肇
  • Organizer: 大会-昆虫機能・利用学 術講演会
  • Place of Presentation: 上田市
  • Year and Date: 20121110-11
• [Presentation] カイコTIA-1ホモローグとバキュロウイルス核酸結合タンパク質との相互作用に関する研究 (2012)
  • Author(s): 小谷英治・武藤清佳・森 肇
  • Organizer: 平成24年度日本蚕糸学会合同支部大会-昆虫機能・利用学 術講演会
  • Place of Presentation: 上田市
  • Year and Date: 20121110-11
• [Remarks]
  • URL: http://kit-ibrc.com/research/
• [Remarks]
  • URL: https://sites.google.com/site/konchuukougaku/
• [Remarks]
  • URL: http://www.bio.kit.ac.jp/chromosome/INDEX.html
• [Remarks]
  • URL: https://sites.google.com/site/ibmrcdrosophila/home