Characteristics of three cytosolic glutamine synthetases and two NADH-glutamate synthase in ammonium assimilation and nitrogen utilization rice

2013 Fiscal Year Final Research Report

• Project/Area Number: 22248005
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant nutrition/Soil science
• Research Institution: Tohoku University
• Principal Investigator: YAMAYA Tomoyuki 東北大学, (連合)農学研究科(研究院), 教授 (30144778)
• Project Period (FY): 2010-04-01 – 2014-03-31
• Keywords: イネ / 窒素利用 / グルタミン代謝 / 逆遺伝学 / メタボローム

Research Abstract

Reverse genetics and spatial expression of genes encoding the three isoenzymes of cytosolic glutamine synthetase (GS1;1, GS1;2, and GS1;3) and two NADH-glutamatesynthases (NADH-GOGAT1 and NADH-GOGAT2) were characterized in rice. OsGS1;2 and OsNADH-GOGAT1 are important in the primary assimilation of ammonium taken up by rice roots. On the other hand, GS1;1 and NADH-GOGAT2 could be important in remobilization of nitrogen during natural senescence. Metabolite profiling data showed a crucial role of GS1;1 in coordinating metabolic balance in rice. Expression of OsGS1:3 was spikelet-specific, indicating that it is probably important in grain ripening and/or germination. Thus, these isoenzymes seem to possess distinct and non-overlapping functions and none was able to compensate for the individual function of another.

Research Products

• [Journal Article] Evidences supporting distinct functions of three cytosolic glutamine synthetases and two NADH-glutamate synthases in rice (2014)
  • Author(s): Yamaya, T. and Kusano M.
  • Journal Title: Journal of Experimental Botany Volume: (in press)
  • Peer Reviewed
• [Journal Article] NADH-dependent glutamate synthase plays a crucial role in assimilating ammonium in Arabidopsis root (2014)
  • Author(s): Konishi, N., Ishiyama, K., Matsuoka, K., Maru, I., Hayakawa, T., Yamaya, T. and Kojima, S.
  • Journal Title: Physiologia Plantarum Volume: (in press)
  • Peer Reviewed
• [Journal Article] Cytosolic glutamine synthetase1 ; 2 is responsible for the primary assimilation of ammonium in rice roots (2013)
  • Author(s): Funayama, K.^$, Kojima, S.^$, Tabuchi-Kobayashi, M., Sawa, Y., Nakayama, Y., Hayakawa, T. and Yamaya, T
  • Journal Title: Plant and Cell Physiology (selected a highlight paper) Volume: 54(6) Pages: 934-943
  • Peer Reviewed
• [Journal Article] Metabolomics data reveal a crucial role of cytosolic glutamine synthetase 1 ; 1 in coordinating metabolic balance in rice (2011)
  • Author(s): Kusano, M., Tabuchi, M., Fukushima, A., Funayama, K., Diaz, C., Kobayashi, M., Hayashi, N., Tsuchiya N. Y., Takahashi, H., Kamata, A., Yamaya, T., and Saito, K
  • Journal Title: Plant Journal Volume: 66 Pages: 456-466
  • Peer Reviewed
• [Journal Article] Disruption of a novel NADH-glutamate synthase2 gene caused marked reduction in spikelet number of rice (2011)
  • Author(s): Tamura, W., Kojima, S., Toyokawa, A., Watanabe, H., Tabuchi-Kobayashi, M., Hayakawa, T. and Yamaya, T.
  • Journal Title: Frontiers in Plant Science (on line journal) Volume: 2 Pages: 57
  • DOI: 10.3389/fpls.2011.00057
  • Peer Reviewed
• [Presentation] シロイヌナズナは2 つの低親和型グルタミン合成酵素を使い分けて根でアンモニウムを同化する (2014)
  • Author(s): 小西範幸,石山敬貴,丸郁美,松岡香矢,早川俊彦,山谷知行,小島創一
  • Organizer: 第55回日本植物生理学会年会
  • Place of Presentation: 富山大学
  • Year and Date: 20140318-20
• [Presentation] Strigolactone-independent reduction of tiller number in rice mutant lacking cytosolic glutamine synthetase1 ; 2 or NADH-glutamate synthase1 (2014)
  • Author(s): Ohashi, M., Ishiyama, K., Hanada, A., Kojima, S., Hayakawa, T., Seto, Y., Yamaguch, Y., Yamaya, T.
  • Organizer: 第55回日本植物生理学会年会
  • Place of Presentation: 富山大学
  • Year and Date: 20140318-20
• [Presentation] The proper use of glutamine produced by cytosolic GS1 ; 1 is critical for metabolic and organelle homeostasis in rice (2014)
  • Author(s): Kusano, M., Fukushima, A., Funayama, K., Tabuchi-Kobayashi, M., Nishizawa, T., Kobayashi, M., Wakazaki, M., Sato, M., Toyooka, M., Osanai-Kondo, K., Utsumi, Y., Seki, S., Kojima, S., Saito, S., Yamaya, T.
  • Organizer: 第55回日本植物生理学会年会
  • Place of Presentation: 富山大学
  • Year and Date: 20140318-20
• [Presentation] イネ維管束ネットワーク上のショ糖転流と顆粒成長モデル (2014)
  • Author(s): 佐竹暁子,関元秀,Francois Feugier,池田真由子,北野英己,Xian-Jun Song,芦苅基行,中村巴瑠花,石山敬貴,山谷知行
  • Organizer: 第55回日本植物生理学会年会シンポジウム「環境変動に対する植物の生存成長戦略:総合研究の新展開」
  • Place of Presentation: 富山大学
  • Year and Date: 2014-03-20
  • Invited
• [Presentation] イネ根におけるアンモニウム態窒素吸収の負の制御の新理解 (2014)
  • Author(s): 早川俊彦、山谷知行
  • Organizer: 第55回日本植物生理学会年会シンポジウム「植物の三大栄養素(N-P-K)の感知と利用の新理解」
  • Place of Presentation: 富山大学
  • Year and Date: 2014-03-18
  • Invited
• [Presentation] Distinct function of GS1 and NADH-GOGAT isoenzymes in rice (2013)
  • Author(s): Yamaya, T.
  • Organizer: Second International Symposium on the Nitrogen Nutrition of Plants
  • Place of Presentation: Puerto Varas, Chile
  • Year and Date: 20131118-22
  • Invited
• [Presentation] The study of two cytosolic glutamine synthetase isoforms of rice using reverse genetic, metabolite and transcript profiling approaches and microscopic analysis (2013)
  • Author(s): Kusano, M., Fukushima, A., Funayama, K., Tabuchi-Kobayashi, M., Nishizawa, T., Kobayashi, M., Waakazaki, M., Sato, M., Toyooka, K., Osanai-Kondo, K., Utsumi, Y., Seki, M., Kojima, S., Yamaya, T., Saito, K.
  • Organizer: Second International Symposium on the Nitrogen Nutrition of Plants
  • Place of Presentation: Puerto Varas, Chile
  • Year and Date: 20131118-22
  • Invited
• [Presentation] Rice novel protein kinase involved in regulation of ammonium uptake into roots under high concentration of external ammonium (2013)
  • Author(s): Hayakawa, T., Obara, M., Taniai, A., Sawa, Y., Ishizawa, J., Kojima, S., Yamaya, T.
  • Organizer: Second International Symposium on the Nitrogen Nutrition of Plants
  • Place of Presentation: Puerto Varas, Chile
  • Year and Date: 20131118-22
• [Presentation] シロイヌナズナサイトゾル型グルタミン合成酵素は根でアンモニウムによって誘導されアンモニウムを同化する (2013)
  • Author(s): 小西範幸、早川俊彦、山谷知行、小島創一
  • Organizer: 日本農芸化学会2013年度大会
  • Place of Presentation: 東北大学
  • Year and Date: 20130325-27
• [Presentation] シロイヌナズナNADH グルタミン酸合成酵素の根におけるアンモニウム同化の重要性 (2013)
  • Author(s): 小西範幸、松岡香矢、丸郁美、早川俊彦、山谷知行、小島創一
  • Organizer: 第54回日本植物生理学会年会
  • Place of Presentation: 岡山大学
  • Year and Date: 20130321-23
• [Presentation] シロイヌナズナ環境型におけるアンモニウム栄養利用の遺伝的な差異 (2013)
  • Author(s): 松岡香矢、佐々木和弘、大岩優喜、丸郁美、早川俊彦、佐藤雅志、山谷知行、小島創一
  • Organizer: 第54回日本植物生理学会年会
  • Place of Presentation: 岡山大学
  • Year and Date: 20130321-23
• [Presentation] Evidence to support a function of glutamine synthetase1 ; 2 in the primary assimilation of ammonium ions in rice roots (2012)
  • Author(s): Funayama, K., Tabuchi-Kobayashi, M., Sawa, Y., Kojima, S., Hayakawa, T. and Yamaya, T.
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都産業大学
  • Year and Date: 20120316-18
• [Presentation] イネ根における新規プロテインキナーゼ(OsACTPK1)のアンモニウム吸収抑制への関与 (2012)
  • Author(s): 谷合彰子、澤勇己、小原実広、吉成晃、小島創一、山谷知行、早川俊彦
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都産業大学
  • Year and Date: 20120316-18
• [Presentation] 酵母スプリットユビキチン法によるアンモニウム輸送担体と細胞質型グルタミン合成酵素の相互作用の解析 (2012)
  • Author(s): 松岡香矢、大岩優喜、早川俊彦、山谷知行、小島創一
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都産業大学
  • Year and Date: 20120316-18
• [Presentation] Reduction of the primary assimilation of ammnonium ions and panicle number in rice mutants lacking cytosolic glutamine synthetase1 ; 2 (2011)
  • Author(s): Funayama, K., Tabuchi-Kobayashi, M., Yawa, Y., Kojima, S., Hayakawa, T., Yamaya, T.
  • Organizer: International Symposium "Strategies of Plants against Global Environmental Change"
  • Place of Presentation: Kurashiki, Okayama, Japan (poster)
  • Year and Date: 20111208-10
• [Presentation] Interplay between ammonium transporter and glutamine synthethase in Arabidopsis (2011)
  • Author(s): Tanaka-Matsuoka, T., Oiwa, Y., Hayakawa, T., Yamaya, T., Kojima, S.
  • Organizer: International Symposium "Strategies of Plants against Global Environmental Change"
  • Place of Presentation: Kurashiki, Okayama, Japan (poster)
  • Year and Date: 20111208-10
• [Presentation] 硫酸イオントランスポーターSULTR2 ; 1 の根における硫黄欠乏応答における3'非転写領域の役割 (2011)
  • Author(s): 丸山明子、井上恵理、高橋晶子、斉藤和季、山谷知行、高橋秀樹
  • Organizer: 日本土壌肥料学会2011年度つくば大会
  • Year and Date: 20110808-19
• [Presentation] Identification and characterization of qRL6.1, a major QTL for root length of seedlings hydroponically grown in rice (2011)
  • Author(s): Obara, M., Fukuta, Y., Sato, T., Yano, M., Yamaya, T.
  • Organizer: The 7^<th> Asian Crop Science Association Conference
  • Place of Presentation: Bogor, Indonesia
  • Year and Date: 2011-09-28
• [Presentation] Oryza glaberrina の挿入置換系統群を用いた、イネ根長のQTL 検出と検証 (2011)
  • Author(s): 小原実広、福田善通、早川俊彦、山谷知行
  • Organizer: 日本土壌肥料学会2011年度つくば大会
  • Place of Presentation: エポカルつくば
  • Year and Date: 2011-08-09
• [Remarks]
  • URL: http://www.agri.tohoku.ac.jp/cellbio/index-j.htm