2013 Fiscal Year Final Research Report
Construction of a protozoan infection therapeutic vaccine development platform technology using antigen-displaying baculovirus
| Project/Area Number |
22248009
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied biochemistry
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| Research Institution | Shizuoka University |
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Principal Investigator |
PARK Enoch Y. 静岡大学, グリーン科学技術研究所, 教授 (90238246)
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| Project Period (FY) |
2010-04-01 – 2014-03-31
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| Keywords | カイコ / ワクチン / 抗原提示 / バキュロウイルス / バイオテクノロジー |
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| Research Abstract |
In this study, we build the foundation of component vaccine development of neosporosis. Five candidate antigens were successfully expressed and purified in silkworm. Bombyx mori nucleopolyhedrovirus (BmNPV) particles displaying Neospora caninum antigens (NcSAG1, NcSRS2 and NcMIC3) purified from silkworm larvae were constructed to vaccinate mice against N. caninum. Antigen-specific IgG2a was predominantly produced in mice by immunization with NcSAG1-displaying BmNPV particles compared to IgG1, and induction of IFN-gamma was dominant, indicating that antigen-displaying BmNPV particles can elicit a Th1 immune response in mice. Semi-quantitative PCR analysis revealed that immunization with each antigen-displaying BmNPV particle purified from silkworms partially protected mice from cerebral N. caninum infection. These results suggest that antigen-displaying BmNPV particles purified from silkworms can provide an alternative method of a new generation of N. caninum vaccines.
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