The molecular mechanisms of environmental stress responses by redox regulation in plants

2013 Fiscal Year Final Research Report

• Project/Area Number: 22248042
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied molecular and cellular biology
• Research Institution: Kinki University
• Principal Investigator: SHIGEOKA Shigeru 近畿大学, 農学部, 教授 (80140341)
• Co-Investigator(Kenkyū-buntansha): YOSHIMURA Kazuya 中部大学, 応用生物学部, 准教授 (90379561)
• Project Period (FY): 2010-05-31 – 2014-03-31
• Keywords: レドックス / 酸化的シグナル / 環境ストレス応答 / 活性酸素種 / Nudix hydrolase / ヌクレオシド-2リン酸

Research Abstract

To comprehensively understand "the molecular mechanisms of environmental stress responses by redox regulation in plants", the molecular mechanisms of (1) oxidative signaling via ROS produced from chloroplasts and (2) physiological roles of Nudix hydrolases were analyzed. We found that (1) oxidative signaling pathways modulated by the genes whose expressions are specifically regulated by chloroplastic H202 and (2) Nudix hydrolases regulate stress responsive mechanisms via metabolisms of various nucleoside-2 phosphate derivatives.

Research Products

• [Journal Article] Transient expression analysis reveals importance of the VTC2 expression level in light/dark regulation of ascorbate biosynthesis in Arabidopsis (2014)
  • Author(s): Yoshimura, K., Nakane, T., Kume, S., Shiomi, Y., Maruta, T., Ishikawa, T. and Shigeoka, S.
  • Journal Title: Biosci.Biotechnol. Biochem Volume: 78 Pages: 60-66
  • DOI: 10.1080/09168451.2014.877831
  • Peer Reviewed
• [Journal Article] Ferulic acid 5-hydroxylase 1 is essential for expression of anthocyanin biosynthesis-associated genes and anthocyanin accumulation under photooxidative stress in Arabidopsis (2014)
  • Author(s): Maruta, T., Noshi, M., Nakamura, M., Matsuda S., Tamoi, M., Ishikawa, T. and Shigeoka, S.
  • Journal Title: Plant Sci Volume: 219-220 Pages: 61-68
  • DOI: 10.1016/j.plantsci.2014.01.003
  • Peer Reviewed
• [Journal Article] Nudix hydrolase ファミリーによるビタミン補酵素型の代謝制御 (2013)
  • Author(s): 吉村和也、伊藤大輔、丸田隆典、重岡 成
  • Journal Title: ビタミン Volume: 87 Pages: 1-12
  • URL: http://mol.medicalonline.jp/library/journal/download?GoodsID=cr9vitam/2013/008701/001&name=0001-0012j&UserID=163.51.106.144
• [Journal Article] Enzymatic and molecular characterization of Arabidopsis ppGpp pyrophosphohydrolase, AtNUDX26 (2012)
  • Author(s): Ito, D., Kato, T., Maruta, T., Tamoi, M., Yoshimura, K., and Shigeoka, S.
  • Journal Title: Biosci.Biotechnol. Biochem Volume: 76 Pages: 2236-2241
  • DOI: 10.1271/bbb.120523
  • Peer Reviewed
• [Journal Article] Cytosolic ascorbate peroxidase 1 protects organelles against oxidative stress by wounding- and jasmonate-induced H2O2 in Arabidopsis plants (2012)
  • Author(s): Maruta, T., Inoue, T., Noshi, M., Tamoi, M., Yabuta, Y., Yoshimura, K., Ishikawa, T. and Shigeoka, S.
  • Journal Title: Biochimica Biophysica Acta Volume: 1820 Pages: 1901-1907
  • DOI: 10.1016/j.bbagen.2012.08.003
  • Peer Reviewed
• [Journal Article] An Arabidopsis FAD pyrophosphohydrolase, AtNUDX23, is involved in the flavin homeostasis (2012)
  • Author(s): Maruta, T., Yoshimoto, T., Ito, D., Ogawa, T., Tamoi, M., Yoshimura, K.,and Shigeoka, S.
  • Journal Title: Plant Cell Physiology Volume: 53 Pages: 1106-1116
  • DOI: 10.1093/pcp/pcs054
  • Peer Reviewed
• [Journal Article] H2O2-triggered retrograde signaling from chloroplasts to nucleus plays a specific role in the response to stress (2012)
  • Author(s): Maruta, T., Noshi, M., Tanouchi, A., Tamoi, M., Yabuta, Y., Yoshimura, K.,Ishikawa, T., and Shigeoka, S.
  • Journal Title: J. Biol. Chem Volume: 287 Pages: 11717-11729
  • DOI: 10.1074/jbc.M111.292847
  • Peer Reviewed
• [Journal Article] A Comparative analysis of molecular characteristics of Arabidopsis CoA pyrophosphohydrolases, AtNUDX11, 15, and 15a (2012)
  • Author(s): Ito, D., Yoshimura, K., Ishikawa, K., Ogawa, T., Maruta, T.,and Shigeoka S.
  • Journal Title: Biosci.Biotechnol. Biochem Volume: 76 Pages: 139-147
  • DOI: 10.1271/bbb.110636
  • Peer Reviewed
• [Journal Article] Arabidopsis NADPH oxidases, AtrbohD and AtrbohF, are essential for jasmonic acid-induced expression of genes regulated by MYC2 transcription factor (2011)
  • Author(s): Maruta, T., Inoue, T., Tamoi, M., Yabuta, Y., Yoshimura, K., Ishikawa, T. and Shigeoka, S.
  • Journal Title: Plant Science Volume: 180 Pages: 655-660
  • DOI: 10.1016/j.plantsci.2011.01.014
  • Peer Reviewed
• [Journal Article] Understanding oxidative stress and antioxidant functions in order to enhance photosynthesis (2011)
  • Author(s): Foyer, CH. and Shigeoka, S.
  • Journal Title: Plant Physiology Volume: 155 Pages: 93-100
  • DOI: 10.1104/pp.110.166181
  • Peer Reviewed
• [Journal Article] Distinct regulation of Arabidopsis ADP-ribose/NADH pyrophosphohydrolases, AtNUDX6 and 7, inbiotic and abiotic stress responses (2010)
  • Author(s): Ishikawa, K., Yoshimura, K., Ogawa, T. and Shigeoka, S.
  • Journal Title: Plant Signal.Behav Volume: 72 Pages: 839-841
  • DOI: 10.1104/pp.109.140442
  • Peer Reviewed
• [Journal Article] AtNUDX6, an ADP-ribose/NADH pyrophosphohydrolase in Arabidopsis, positively regulates NPR1 dependent salicylic acid signaling (2010)
  • Author(s): Ishikawa, K., Yoshimura, K., Harada, K., Fukusaki, E., Ogawa, T., Tamoi,M. and Shigeoka, S.
  • Journal Title: Plant Physiology Volume: 152 Pages: 2000-2012
  • DOI: 10.1104/pp.110.153569
  • Peer Reviewed
• [Journal Article] Arabidopsis chloroplastic ascorbate peroxidase isoenzymes play a dual Role in photoprotection and gene regulation under photooxidative stress (2010)
  • Author(s): Maruta, T., Tanouchi, A., Tamoi, M., Yabuta, Y., Yoshimura, K.,Ishikawa, T. and Shigeoka, S.
  • Journal Title: Plant Cell Physiology Volume: 51 Pages: 190-200
  • DOI: 10.1093/pcp/pcp177
  • Peer Reviewed
• [Journal Article] 高等植物におけるNudix hydrolase ファミリーの多様な生理機能 -ヌクレオシド2-リン酸類縁体の代謝を介した細胞応答制御 (2010)
  • Author(s): 吉村和也、石川和也、重岡 成
  • Journal Title: 化学と生物 Volume: 48 Pages: 305-308
  • URL: http://www.jstage.jst.go.jp/article/kagakutoseibutsu/48/5/48_5_305/_article/-char/ja/
• [Presentation] 葉緑体型 NADPH 加水分解酵素(AtNUDX19)はストレス / ホルモン応答のバランス制御に関与する (2014)
  • Author(s): 丸田隆典
  • Organizer: 第55回日本植物生理学会年回
  • Place of Presentation: 富山
  • Year and Date: 2014-03-18
• [Presentation] Nudix hydrolase(AtNUDX6 および7)による細胞内NADH 代謝を介した遺伝子発現制御 (2013)
  • Author(s): 村本亘平
  • Organizer: 日本分子生物学会
  • Place of Presentation: 兵庫
  • Year and Date: 2013-12-03
• [Presentation] 葉緑体型NADPH 加水分酵素(AtNUDX19)によるNADPH ステータス変化を介したストレス/ホルモン応答の制御 (2013)
  • Author(s): 辻村昌希
  • Organizer: 日本農芸化学会2013年度大会
  • Place of Presentation: 宮城
  • Year and Date: 2013-03-25
• [Presentation] FAD 加水分解酵素による植物フラビン代謝の制御機構 (2013)
  • Author(s): 吉村和也
  • Organizer: 第54回日本植物生理学会年会シンポジウム
  • Place of Presentation: 岡山
  • Year and Date: 2013-03-21
• [Presentation] レドックス制御を介した環境ストレス応答と分子育種 (2012)
  • Author(s): 重岡 成
  • Organizer: 第30回日本植物細胞分子生物学会(生駒)大会・シンポジウム
  • Place of Presentation: 奈良
  • Year and Date: 2012-08-03
  • Invited
• [Presentation] シロイヌナズナ葉緑体型Nudixhydrolase(AtNUDX19)によるNADPH 代謝を介した強光ストレス応答機構 (2012)
  • Author(s): 吉村和也
  • Organizer: 日本農芸化学会2012年度大会
  • Place of Presentation: 京都
  • Year and Date: 2012-03-23
• [Presentation] H_2O_2 signaling from chloropla sts to nucleus plays a specific role in the response to stress (2011)
  • Author(s): 丸田隆典
  • Organizer: 10^<th> Internatio nal Conference on Reactive Oxygen and a nd Nitrogen Species in Plants
  • Place of Presentation: ハンガリー
  • Year and Date: 2011-07-08
• [Presentation] NADPH加水分解酵素(AtNUDX19)が光環境順応制御に果たす役割 (2011)
  • Author(s): 吉村和也
  • Organizer: 日本ビタミン学会第63回大会
  • Place of Presentation: 広島
  • Year and Date: 2011-06-04
• [Presentation] 葉緑体型NADPH 加水分解酵素(AtNUDX19)の植物ホルモンを介したストレス応答への関与 (2011)
  • Author(s): 吉村和也
  • Organizer: 第52回日本植物生理学会年回
  • Place of Presentation: 宮城
  • Year and Date: 2011-03-21
• [Presentation] シロイヌナズナNudix hydrolase, AtNUDX6, による全身獲得抵抗性の制御 (2010)
  • Author(s): 吉村和也
  • Organizer: 日本植物学会第74回大会
  • Place of Presentation: 愛知
  • Year and Date: 2010-09-10
• [Book] ビタミン総合事典5-2-3 植物におけるパントテン酸の生合成 (2010)
  • Author(s): 吉村和也
  • Total Pages: 272-275
  • Publisher: 朝倉書店
• [Remarks] 近畿大学農学部バイオサイエンス学科植物分子生理学研究室ホームページ
  • URL: http://nara-kindai.unv.jp/02gakka/06bio/syokubun/index.html