2013 Fiscal Year Final Research Report
Novel strategy to regulate renal channels and transporters
Project/Area Number |
22249032
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Research Category |
Grant-in-Aid for Scientific Research (A)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Kidney internal medicine
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Research Institution | Tokyo Medical and Dental University |
Principal Investigator |
SASAKI SEI 東京医科歯科大学, 医歯(薬)学総合研究科, 教授 (60170677)
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Project Period (FY) |
2010-04-01 – 2014-03-31
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Keywords | 水・電解質代謝学 / 塩分感受性高血圧 / 遺伝子改変マウス |
Research Abstract |
The purpose of our study was to clarify novel regulatory mechanisms of renal transporters and channels, especially AQP water channels, CLC chloride channels, and SLC12A transporters, and to try to develop new strategy to treat diseases caused by the dysregulation of these transporters and channels. We clarified the molecular pathogenesis of renal cyst formation caused by the deletion of AQP11, identified the agents to rescue endoplasmic reticulum (ER)-retained disease-causing mutant Barttin, and isolated some chemical compounds that inhibited WNK-OSR1/SPAK-SLC12A signaling.
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[Journal Article] Development of enzyme-linked immunosorbent assays for urinary thiazide-sensitive Na-Cl cotransporter (NCC) measurement2013
Author(s)
Isobe K, Mori T, Asano T, Kawaguchi H, Nonoyama S, Kumagai N, Kamada F, Morimoto T, Hayashi M, Sohara E, Rai T, Sasaki S, Uchida S
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Journal Title
Am J Physiol Renal Physiol
Volume: 305
Pages: F1374-F1381
Peer Reviewed
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[Journal Article] Impaired KLHL3-mediated ubiquitination of WNK4 causes human hypertension2013
Author(s)
Wakabayashi M, Mori T, Isobe K, Sohara E, Susa K, Araki Y, Chiga M, Kikuchi E, Nomura N, Mori Y, Matsuo H, Murata T, Nomura S, Asano T, Kawaguchi H, Nonoyama S, Rai T, Sasaki S, Uchida S
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Journal Title
Cell Rep
Volume: 3
Pages: 858-868
Peer Reviewed
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[Journal Article] Dietary salt intake regulates WNK3-SPAK-NKCC1 phosphorylation cascade in mouse aorta through angiotensin II2013
Author(s)
Zeniya M, Sohara E, Kita S, Iwamoto T, Susa K, Mori T, Oi K, Chiga M, Takahashi D, Yang SS, Lin SH, Rai T, Sasaki S, Uchida S
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Journal Title
Hypertension
Volume: 62
Pages: 872-878
Peer Reviewed
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[Journal Article] Phosphorylation of Na-Cl cotransporter by OSR1 and SPAK kinases regulates its ubiquitination2012
Author(s)
Hossain Khan MZ, Sohara E, Ohta A, Chiga M, Inoue Y, Isobe K, Wakabayashi M, Oi K, Rai T, Sasaki S, Uchida S
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Journal Title
Biochem. Biophys. Res. Commun
Volume: 425
Pages: 456-461
Peer Reviewed
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[Journal Article] Effect of heterozygous deletion of WNK1 on the WNK-OSR1/ SPAK-NCC/NKCC1/NKCC2 signal cascade in the kidney and blood vessels2012
Author(s)
Susa K, Kita S, Iwamoto T, Yang SS, Lin SH, Ohta A, Sohara E, Rai T, Sasaki S, Alessi DR, Uchida S
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Journal Title
Clin. Exp. Nephrol
Volume: 16
Pages: 530-538
Peer Reviewed
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[Journal Article] Generation and analyses of R8L barttin knockin mouse2011
Author(s)
Nomura N, Tajima M, Sugawara N, Morimoto T, Kondo Y, Ohno M, Uchida K, Mutig K, Bachmann S, Soleimani M, Ohta E, Ohta A, Sohara E, Okado T, Rai T, Jentsch TJ, Sasaki S, Uchida S
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Journal Title
Am. J. Physiol. Renal. Physiol
Volume: 301
Pages: F297-307
Peer Reviewed
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[Journal Article] Acute insulin stimulation induces phosphorylation of the Na-Cl cotransporter in cultured distal mpkDCT cells and mouse kidney2011
Author(s)
Sohara E, Rai T, Yang SS, Ohta A, Naito S, Chiga M, Nomura N, Lin SH, Vandewalle A, Ohta E, Sasaki S, Uchida S
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Journal Title
PLoS One
Volume: 6
Pages: e24277
Peer Reviewed
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[Presentation] Impaired KLHL3-mediated ubiquitination of WNK4 activates OSR1 and SPAK kinases-NaCl cotransporter (NCC) signaling and causes hypertension2013
Author(s)
Wakabayashi M, Mori T, Isobe K, Sohara E, Susa K, Araki Y, Chiga M, Kikuchi E, Nomura N, Mori Y, Rai T, Sasaki S, Uchida S
Organizer
The 46^<st> Annual Meeting of American Society of Nephrology
Place of Presentation
Atlanta
Year and Date
2013-11-09
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[Presentation] WNK3 Regulates Blood Pressure Through the Regulation of Vascular OSR1/SPAK-NKCC1 Phosphorylation Cascade2013
Author(s)
Zeniya M, Sohara E, Kita S, Iwamoto T, Susa K, Mori T, Oi K, Chiga M, Takahashi D, Yang SS, Lin SH, Rai T, Sasaki S, Uchida S
Organizer
The 46^<th> Annual Meeting of American Society of Nephrology
Place of Presentation
Atlanta
Year and Date
2013-11-09
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