Studies on signal activating systems induced by an anorexigenicappetitekine, Nesfatin-1

2012 Fiscal Year Final Research Report

• Project/Area Number: 22249038
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Endocrinology
• Research Institution: Gunma University
• Principal Investigator: MORI Masatomo 群馬大学, 大学院・医学系研究科, 教授 (80174382)
• Co-Investigator(Kenkyū-buntansha): OKADA Shuichi 群馬大学, 医学部, 講師 (20260474) ; HASHIMOTO Koshi 群馬大学, 大学院・医学系研究科, 助教 (30396642) ; YADA Toshihiko 自治医科大学, 医学部, 教授 (60166527) ; YAMADA Masanobu 群馬大学, 大学院・医学系研究科, 准教授 (90261833)
• Project Period (FY): 2010 – 2012
• Keywords: Nesfatin-1 / 肥満 / シグナル伝達 / 摂食抑制 / 脂肪細胞

Research Abstract

Nesfatin-1 administration promoted a dose-dependent increase in CRE reporteractivities inmouse NB41A3cells,and Nesfatin-1 bound plasmamembranes derived frommouse hypothalami and NB41A3 cells in a high affinity manner. Using a CRE reporter assay ineachof 49 GPCRs, wetried to identify a receptorspecific to Nesfatin-1, but these efforts resulted in failure. Troglitazone, an activator for PPARγ, possessed a significantstabilizing activity of NUCB2 gene mRNA, and Nesfatin-1 activated EGF signaling tosuppress adipogenesis.

Research Products

• [Journal Article] Glucose and insulin induce Ca^<2+> signaling in nesfatin-1 neurons in the hypothalamic paraventricular nucleus (2012)
  • Author(s): Gantulga D, Maejima Y, Nakata M, Yada T
  • Journal Title: Biochem Biophys Res Commun Volume: 420(4) Pages: 811-815
  • Peer Reviewed
• [Journal Article] Nesfatin-1 Induces the Phosphorylation Levels of cAMP Response Element-Binding Protein for Intracellular Signaling in a Neural Cell Line (2012)
  • Author(s): Ishida E, Hashimoto K, Shimizu H, Okada S, Satoh T, Kato I, Yamada M, Mori M
  • Journal Title: PLoS One Volume: 7 Pages: e50918
  • Peer Reviewed
• [Journal Article] Secreted Nucleobindin-2 inhibits 3T3-L1 adipocyte differentiation (2012)
  • Author(s): Tagaya Y, Osaki A, Miura A, Okada S, Ohshima K, Hashimoto K, Yamada M, Satoh T, Shimizu H, Mori M
  • Journal Title: Protein Pept. Lett Volume: 19 Pages: 997-1004
  • Peer Reviewed
• [Journal Article] Nucleobindin-2 is a positive modulator of EGF-dependent signals leading to enhancement of sell growth and suppression of adipocyte differentiation (2012)
  • Author(s): Tagaya Y, Miura A, Okada S, Ohshima K, Mori M.
  • Journal Title: Endocrinology Volume: 153 Pages: 3308-3319
  • Peer Reviewed
• [Journal Article] Nesfatin-1 enhances glucose-induced insulin secretion bypromoting Ca^<2+> influx through L-type channels in mouse islet b-cells (2011)
  • Author(s): Nakata M, Manaka K, Yamamoto S, Mori M, Yada T
  • Journal Title: Endocr J Volume: 58(4) Pages: 305-313
  • Peer Reviewed
• [Journal Article] Peripheral oxytocin treatment ameliorates obesity by reducing food intake and visceral fat mass (2011)
  • Author(s): Maejima Y, Iwasaki Y, Yamahara Y, Kodaira M, Sedbazar U, Yada T
  • Journal Title: Aging (Albany NY) Volume: 3(12) Pages: 1169-1177
  • Peer Reviewed
• [Journal Article] Troglitazone, a ligand of peroxisome proliferator-activatedreceptor-g, stabilizes NUCB2 (Nesfatin)mRNA by activating the ERK1/2 pathway:isolation and characterization of the human NUCB2 gene (2010)
  • Author(s): Yamada M, Horiguchi K, Umezawa R, Hashimoto K, Satoh T, Ozawa A,Shibusawa N, Monden T, Okada S, Shimizu H, Mori M
  • Journal Title: Endocrinology Volume: 151 Pages: 2494-2503
  • Peer Reviewed
• [Journal Article] Stressor-responsive central nesfatin-1 activates corticotropin-releasing hormone, noradrenaline and serotonin neurons and evokes hypothalamic-pituitary-adrenal axis (2010)
  • Author(s): Yoshida N, Maejima Y, Sedbazar U, Ando A, Kurita H, Damdindorj B, Takano E, Gantulga D, Iwasaki Y, Kurashina T, Onaka T, Dezaki K, Nakata M, Mori M, Yada T
  • Journal Title: Aging (Albany NY) Volume: 2(11) Pages: 775-784
  • Peer Reviewed
• [Presentation] 摂食抑制ペプチドNesfatin-1の細胞内シグナル伝達機構の解析とその特異的受容体の探索 (2013)
  • Author(s): 橋本貢士、森昌朋, 他
  • Organizer: 第86回日本内分泌学会
  • Place of Presentation: 仙台
  • Year and Date: 2013-04-25