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2012 Fiscal Year Final Research Report

Analysis of transcription factory and epigenomic modification in inflammatory stimulation driven transcription wave

Research Project

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Project/Area Number 22310117
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Genome biology
Research InstitutionThe University of Tokyo

Principal Investigator

WADA Youichiro  東京大学, 先端科学技術研究センター, 特任准教授 (10322033)

Co-Investigator(Kenkyū-buntansha) KANKI Yasuharu  東京大学, 先端科学技術研究センター, 特任研究員 (00534869)
KHORO Takahide  東京大学, 医学部付属病院, 特任助教 (40401046)
OHTA Yoshihiro  東京大学, 先端科学技術研究センター, 特任研究員 (80436592)
Project Period (FY) 2010 – 2012
KeywordsRNA ポリメレース / TNFα / ヒストン修飾 / 内皮細胞
Research Abstract

Chronic inflammation of endothelial cell is the first stage ofatherogenesis. We applied an experimental model using endothelial cells with representative inflammatory stimulant, tumor necrosis factor-alpha (TNFα), and to dissect detailedmechanism of induction and reduction of more than 500 genes. To obtain a comprehensive view of a single transcription cycle caused by TNFα in time course manner, we switched onfive long (>100 kbp) genes with TNFα and monitored genomic localization of nascent RNA, RNA Pol II, insulators, and histone modifications. Activation triggers a wave of transcriptionthat sweeps along the genes, and Pol II tends to stall at cohesin/CTCF binding sites. Chromatin conformation capture (3C), both conventional and quantitative data, revealedco-localization of actively transcribed genes. These results suggested that fine-tuning of TNFα responsive genes is achieved by selected number of transcription complexes, whichprovide platform for both transcription and splic ng. By virtue of whole genome chromatininteraction analysis with paired end tag sequencing (ChIA-PET) detailed chromatin structureanalysis was made possible, and time course data on stimulated endothelium sheds light ondynamic chromatin structure change within half an hour.

  • Research Products

    (45 results)

All 2013 2012 2011 2010

All Journal Article (15 results) Presentation (30 results)

  • [Journal Article] TNF alpha signals through specialized factories where responsive coding and miRNA genes are transcribed.2012

    • Author(s)
      Papantonis, A., Kohro, T., Baboo, S., Larkin, J.D., Deng, B., Short, P., Tsutsumi, S., Taylor, S.,Kanki, Y., Kobayashi, M., Li, G., Poh, H., Ruan,X., Aburatani, H., Ruan, Y., Kodama, T., Wada,Y., and Cook, P. R.
    • Journal Title

      The EMBO journal

      Volume: 31 Pages: 4404-4414

    • DOI

      DOI:10.1038/emboj.2012.288.

  • [Journal Article] Distribution of histone3 lysine 4 trimethylation at T3-responsive loci in the heart during reversible changes in gene expression.2012

    • Author(s)
      Pandya, K., Kohro, T., Mimura, I., Kobayashi, M., *Wada, Y. Kodama, T., and Smithies, O.
    • Journal Title

      Gene Expr

      Volume: 15 Pages: 183-198

  • [Journal Article] Path-preference cellular-automaton model for traffic flow through transit points and its application to the transcription process in human cells.2012

    • Author(s)
      Ohta, Y., Nishiyama, A., Wada, Y., Ruan, Y., Kodama, T., Tsuboi, T., Tokihiro, T., and *Ihara, S.
    • Journal Title

      Phys Rev E Stat Nonlin Soft Matter Phys

      Volume: 86 Pages: 021918.

    • DOI

      DOI:10.1103/PhysRevE.86.021918

  • [Journal Article] Dynamic change of chromatin conformation in response to hypoxia enhances the expression of GLUT3 (SLC2A3) by cooperative interaction of hypoxia-inducible factor 1 and KDM3A.2012

    • Author(s)
      Mimura, I., Nangaku, M., Kanki, Y., Tsutsumi, S., Inoue, T., Kohro, T., Yamamoto, S., Fujita, T., Shimamura, T., Suehiro, J., Taguchi, A., Kobayashi, M., Tanimura, K., Inagaki, T., Tanaka, T., Hamakubo, T., Sakai, J., Aburatani, H., Kodama, T., and Wada, Y.
    • Journal Title

      Molecular and Cellular Biology

      Volume: 32 Pages: 3018-3032

    • DOI

      DOI:10.1128/MCB.06643-11.

  • [Journal Article] Alternative splicing of CD44 mRNA by ESRP1 enhances lung colonization of metastatic cancer cell.2012

    • Author(s)
      Yae T, Tsuchihashi K, Ishimoto T, Motohara T, Yoshikawa M, Yoshida GJ, Wada T, Masuko T,Mogushi K, Tanaka H, Osawa T, Kanki Y, et al
    • Journal Title

      Nature Communications

      Volume: 6;3 Pages: 883

    • DOI

      DOI:10.1038/ncomms1892.

  • [Journal Article] A multi-gene transcriptional profiling approach to the discovery of cell signature markers.2011

    • Author(s)
      Wada, Y., Li, D., Merley, A., Zukauskas, A., Aird, W. C., Dvorak, H. F., and *Shih, S. C.
    • Journal Title

      Cytotechnology

      Volume: 63 Pages: 25-33

  • [Journal Article] Pathophysiological response to hypoxia - from the molecular mechanisms of malady to drug discovery: epigenetic regulation of the hypoxic response via hypoxia-inducible factor and histone modifying enzymes.2011

    • Author(s)
      Mimura, I., Tanaka, T., Wada, Y., Kodama, T., and *Nangaku, M.
    • Journal Title

      Journal of pharmacological sciences

      Volume: 115 Pages: 453-458

  • [Journal Article] Epigenetically coordinated GATA2 binding is necessary for endothelium-specific endomucin expression.2011

    • Author(s)
      Kanki, Y., Kohro, T., Jiang, S., Tsutsumi, S., Mimura, I., Suehiro, J., Wada, Y., Ohta, Y., Ihara, S., Iwanari, H., Naito, M., Hamakubo, T., Aburatani, H., Kodama, T., and *Minami, T.
    • Journal Title

      The EMBO journal

      Volume: 30 Pages: 2582-2595

    • DOI

      DOI:10.1038/emboj.2011.173

  • [Journal Article] Mitochondrial DNA polymerase editing mutation, PolgD257A, reduces the diabetic phenotype of Akita male mice by suppressing appetite.2011

    • Author(s)
      Fox, R., Kim, H. S., Reddick, R. L., Kujoth, G. C., Prolla, T. A., Tsutsumi, S., Wada, Y., Smithies, O., and *Maeda, N.
    • Journal Title

      Proceedings of the National Academy ofSciences of the United States of America

      Volume: 108 Pages: 8779-8784

    • DOI

      DOI:10.1073/pnas.1106344108

  • [Journal Article] ChIP-seq reveals cell type-specific binding patterns of BMP-specific Smads and a novel binding motif.2011

    • Author(s)
      Morikawa M, Kanki Y et al.
    • Journal Title

      Nucleic Acids Research

      Volume: 39 Pages: 8712-27

    • DOI

      DOI:10.1093/nar/gkr572

  • [Journal Article] Sev ere dermatitis with loss of epidermal Langerh ans cells in human and mouse zinc deficiency.2011

    • Author(s)
      Kawamura T, Ohta Y, et al.
    • Journal Title

      The Journal of Clinical Investigation

      Volume: 84 Pages: 722-732

    • DOI

      DOI:10.1172/JCI58618

  • [Journal Article] Cellular-automaton model of the cooperative dynamics of RNA polymerase II during transcription in human cells.2011

    • Author(s)
      Ohta Y, et al.
    • Journal Title

      Physical Review E

      Volume: 84 Pages: 041922-2-15

    • DOI

      DOI:10.1103/PhysRevE.84.041922

  • [Journal Article] Proteomic analysis of native hepatocyte nuclear factor-4alpha (HNF4alpha) isoforms, phosphorylation status, and interactive cofactors.2011

    • Author(s)
      Daigo, K, Kawamura T, Ohta Y, et al.
    • Journal Title

      The Journal of Biological Chemistry

      Volume: 286 Pages: 674-686

  • [Journal Article] Genome-wide approaches reveal functional interleukin-4-inducible STAT6 binding to the vascular cell adhesion molecule 1 promoter.2011

    • Author(s)
      Tozawa H, Kanki Y, Wada Y, et al.
    • Journal Title

      Molecular and Cellular Biology

      Volume: vol.31 Pages: 2196-209

    • DOI

      DOI:10.1128/MCB.01430-10

  • [Journal Article] Active RNA polymerases2010

    • Author(s)
      Papantonis, A., Larkin, J. D., Wada, Y., Ohta, Y., Ihara, S., Kodama, T., and Cook, P. R.
    • Journal Title

      mobile or immobile molecular machines PLoS biology 8

      Volume: e1000419.

  • [Presentation] A wave of nascent transcription on activated big genes in human endothelial cells is suggestive to dynamic chromatin movement caused by transcription factories2013

    • Author(s)
      和田洋一郎
    • Organizer
      第90回日本生理学会大会
    • Place of Presentation
      東京
    • Year and Date
      20130327-0329
  • [Presentation] Comprehensive analyses revealed the epigenetic switch during the endothelial cell differentiation2013

    • Author(s)
      神吉康晴
    • Organizer
      第12回日本再生医療学会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      20130321-0323
  • [Presentation] Comprehensive analyses revealed the epigenetic switch during the endothelial cell differentiation2013

    • Author(s)
      神吉康晴
    • Organizer
      第12回日本再生医療学会
    • Place of Presentation
      横浜
    • Year and Date
      20130321-0323
  • [Presentation] Three Dimensional Chromatin Architecture Correlates with Two Opposite Transcription Factors, which contributes the Cell Specificity2013

    • Author(s)
      神吉康晴
    • Organizer
      The 5th HOPE meeting
    • Place of Presentation
      グランドプリンスホテル新高輪
    • Year and Date
      20130225-0302
  • [Presentation] Two transcription factors mediated opposite chromatin conformation correlates with endothelial cell specificity2012

    • Author(s)
      神吉康晴
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      20121211-14
  • [Presentation] Molecular mechanism about sustained VCAM-1 induction by the transcriptome and epigenomic analyses.2012

    • Author(s)
      神吉康晴
    • Organizer
      第20回日本血管生物医学会学術集会 (The 10th Korea-Japan Joint Symposium on Vascular Biology)
    • Place of Presentation
      徳島あわぎんホール
    • Year and Date
      20121205-1207
  • [Presentation] The initiation step about the changes of transcription factor bindings and histone modifications is involved in sustained gene inductions.2012

    • Author(s)
      神吉康晴
    • Organizer
      Keystone Symposia 2013 Aging and Diseases of Aging
    • Place of Presentation
      Sheraton Miyako Hotel Tokyo
    • Year and Date
      20121022-1027
  • [Presentation] Regulation of the Endothelial-Mesenchymal Transition(EndMT)2012

    • Author(s)
      神吉康晴
    • Organizer
      第71回日本癌学会学術総会
    • Place of Presentation
      札幌
    • Year and Date
      20120919-0921
  • [Presentation] 転写過程の超離散モデリングとシミュレーション2012

    • Author(s)
      大田佳宏
    • Organizer
      京都大学数理解析研究所研究集会
    • Place of Presentation
      京都大学益川ホール
    • Year and Date
      20120820-0822
  • [Presentation] Epigenetic Switch on the Differentiation of Mouse Vascular Endothelial Cells2012

    • Author(s)
      神吉康晴
    • Organizer
      International Society for Stem Cell Research 10th Annual Meeting
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      20120613-0616
  • [Presentation] 心血管系エピゲノム研究を通して得られた転写メカニズムの新しい概念2012

    • Author(s)
      和田洋一郎
    • Organizer
      第六回日本エピジェネティクス研究会年会
    • Place of Presentation
      東京
    • Year and Date
      20120514-0515
  • [Presentation] Epigenetically coordinated GATA2 bindings are necessary for the endothelial cell specificity2012

    • Author(s)
      神吉康晴
    • Organizer
      第六回日本エピジェネティクス研究会年会
    • Place of Presentation
      東京
    • Year and Date
      20120514-0515
  • [Presentation] A wave of nascent transcription on activated big genes in human endothelial cells2012

    • Author(s)
      和田洋一郎
    • Organizer
      Keystone Symposia 2012 Chromatin Dynamics
    • Year and Date
      20120117-22
  • [Presentation] Epigenetic Regulation in Endothelial-cell Specific Genes by Transcription factor GATA2 Binding Regions2012

    • Author(s)
      神吉康晴
    • Organizer
      Keystone Symposia 2012 Epigenomics (J3)
    • Place of Presentation
      Keystone Resort, Keystone, Colorado, USA
    • Year and Date
      20120117-1122
  • [Presentation] ChIP-sequence Reveals Functional Interleukin-4-Inducible STAT6 Binding to the Vascular Cell Adhesion Molecule 1 Promoter2012

    • Author(s)
      神吉康晴
    • Organizer
      第12回東京大学生命科学シンポジウム
    • Place of Presentation
      東京大学
    • Year and Date
      2012-06-30
  • [Presentation] Functional Roles of GATA2 in Vascular Biology2011

    • Author(s)
      神吉康晴
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      20111213-1216
  • [Presentation] Identification of novel MEF2C binding site indispensable for KLF4 induction in endothelial cells by statin treatment.2011

    • Author(s)
      前島崇司、和田洋一郎
    • Organizer
      第19回日本血管生物医学会学術集会
    • Place of Presentation
      東京ステーションコンファレンス、東京
    • Year and Date
      20111208-1210
  • [Presentation] Epigenetic Framework in Vascular Endothelial Cells2011

    • Author(s)
      神吉康晴
    • Organizer
      第19回日本血管生物医学会学術集会
    • Place of Presentation
      東京ステーションカンファレンス、東京都
    • Year and Date
      20111208-1210
  • [Presentation] A wave of nascent transcr iption on activated big genes in human endothelial cells2011

    • Author(s)
      和田洋一郎
    • Organizer
      International Symposi um on the Physicochemical Field for Gen etic Activities
    • Place of Presentation
      淡路夢舞台国際会議場(兵庫県)
    • Year and Date
      20111124-1126
  • [Presentation] Epigenetically Coordinate GATA2 Binding Regions are Necessary for the Determination of the Endothelial Cell Specificity2011

    • Author(s)
      神吉康晴
    • Organizer
      The Second Pacific Symposium on Vascular Biology
    • Place of Presentation
      Jeju Island(Shilla Hotel), Korea
    • Year and Date
      20111030-1102
  • [Presentation] Renewal of histone modification during a wave of transcription caused by inflammatory stimulation in endothelial cells.2011

    • Author(s)
      和田洋一郎
    • Organizer
      第5回日本エピジェネティクス研究会
    • Place of Presentation
      KKRホテル、熊本市
    • Year and Date
      20110519-0520
  • [Presentation] Epigenetically Coordinate GATA2 Cistrome is Necessary for the Det ermination of the Endothelial Cell Spec ificity2011

    • Author(s)
      神吉康晴
    • Organizer
      CDB symposium 2011
    • Place of Presentation
      理化学研究所 発生・再生科学総合 研究センター(兵庫県)
    • Year and Date
      20110314-0315
  • [Presentation] High Density Spatial and Temporal Simulation Reveals Transcriptional Dynamics2011

    • Author(s)
      大田佳宏、Yoshihiro
    • Organizer
      The Pacific Symposium on Biocomputing (PSB) 2011
    • Place of Presentation
      Kona, USA.
    • Year and Date
      20110104-0108
  • [Presentation] 疾患と発生を繋ぐエピゲノムー血管疾患の解明に向けてー2011

    • Author(s)
      神吉康晴
    • Organizer
      Genomic Sciences Research Complex(GSC)七夕ミーティング2011
    • Place of Presentation
      理化学研究所横浜研究所、神奈川県
    • Year and Date
      2011-08-25
  • [Presentation] 超高速シーケンサーが明らかにした転写に伴うダイナミックな染色体構造変化と新たな転写装置の概念2011

    • Author(s)
      和田洋一郎
    • Organizer
      第2回ARTセミナー
    • Place of Presentation
      岡山大学
    • Year and Date
      2011-05-25
  • [Presentation] 動脈硬化誘導刺激に応じて血管内皮細胞で複数遺伝子を同時に転写する装置の同定2010

    • Author(s)
      和田洋一郎
    • Organizer
      第33回日本分子生物学会年会、第83回日本生化学会大会合同大会
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      20101207-1210
  • [Presentation] GATA2 occupancies differ f rom each cell type, which is correlated with epigenetic modifications and gene expression profiles in cell-type speci fic manner2010

    • Author(s)
      神吉康晴
    • Organizer
      第33回日本分子生物学会年会、第83回日本生化学会大会合同大会
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      20101207-1210
  • [Presentation] Epigenetically coordinate GATA2 occupancy is necessary for the determination of the endothelial cell specificity2010

    • Author(s)
      神吉康晴
    • Organizer
      第18回日本血管生物医学会学術集会
    • Place of Presentation
      梅田スカイビル
    • Year and Date
      20101201-1203
  • [Presentation] Epigenetically coordinate GATA2 bindings in endothelium is necess ary for the determination of the endoth elial cell specificity2010

    • Author(s)
      神吉康晴
    • Organizer
      2010年度がん若手ワークショップ
    • Place of Presentation
      アートランドホテル蓼科(長野県)
    • Year and Date
      20100901-0904
  • [Presentation] 血管内皮細胞における転写因子GATA2の機能解析2010

    • Author(s)
      神吉康晴
    • Organizer
      第22回高遠シンポジウ ム
    • Place of Presentation
      高遠さくらホテル
    • Year and Date
      20100819-0820

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Published: 2014-08-29  

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