2014 Fiscal Year Final Research Report
Functional analysis of mouse noncoding RNAs by a rapid gene disruption method
| Project/Area Number |
22310118
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Genome biology
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| Research Institution | Nara Medical University (2012-2013)
Osaka University (2010-2011) |
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Principal Investigator |
HORIE Kyoji 奈良県立医科大学, 医学部, 教授 (30333446)
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| Project Period (FY) |
2010-04-01 – 2015-03-31
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| Keywords | ES細胞 / ゲノム / 遺伝子 / non-coding RNA / 変異体 |
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| Outline of Final Research Achievements |
Recent researches demonstrated that most regions of the mammalian genome such as human and mouse are transcribed. Furthermore, most of such transcripts are noncoding RNAs. However, speculation of the function of noncoding RNAs from nucleotide sequences is difficult. We recently developed a method to rapidly generate mutant mouse ES cells. In the present study, we generated mutant mouse ES cell lines using this method and conducted systematic functional analysis of noncoding RNAs.
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| Free Research Field |
分子生物学
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