2012 Fiscal Year Final Research Report
Comprehensive identification of caspase-dependent signal transduction pathway using cell-free protein array
| Project/Area Number |
22310127
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
System genome science
|
|---|
| Research Institution | Ehime University |
|---|
Principal Investigator |
SAWASAKI Tatsuya 愛媛大学, 無細胞生命科学工学研究センター, 教授 (50314969)
|
|---|
| Project Period (FY) |
2010 – 2012
|
| Keywords | 蛋白質ネットワーク |
|---|
| Research Abstract |
Using a cell-free protein array constructed by a wheat cell-free protein synthesis system, comprehensive screening of caspase-3-cleaved protein kinases was carried out. Newly 30 protein kinases were found as substrates of caspase-3. Almost of cleavage sites were located on N- or C-terminal region flaking with regulatory domain. Actually protein function of some substrates was regulated by the cleavage of caspse-3.
|
Research Products
(62 results)
-
-
[Journal Article] Novel approach to identifying autoantibodies in rheumatoid synovitis with a biotinylated human autoantigen library and the enzyme-labeled antigen method2013
Author(s)
Mizutani Y, Matsuoka K, Takeda H, Shiogama K, Inada KI, Hayakawa K, Yamada H, Miyazaki T, Sawasaki T, Endo Y, Tsutsumi Y.
-
Journal Title
J Immunol Methods
Volume: 387
Pages: 57-70
Peer Reviewed
-
[Journal Article] Interferon-Induced SCYL2 Limits Release of HIV-1 by Triggering PP2A-Mediated Dephosphorylation of the Viral Protein Vpu.2012
Author(s)
Miyakawa K, Sawasaki T, Matsunaga S, Tokarev A, Quinn G, Kimura H, Nomaguchi M, Adachi A, Yamamoto N, Guatelli J, Ryo A
-
Journal Title
Sci Signal
Volume: 5
Pages: ra73
Peer Reviewed
-
-
-
[Journal Article] The molecular mechanism of apoptosis upon caspase-8 activation: Quantitative experimental validation of a mathematical model2012
Author(s)
Kominami K, Nakabayashi J, Nagai T, Tsujimura Y, Chiba K, Kimura H, Miyawaki A, Sawasaki T, Yokota H, Manabe N, Sakamaki K
-
Journal Title
Biochim Biophys Acta.
Volume: 1823
Pages: 1825-1840
Peer Reviewed
-
[Journal Article] Molecular and enzymatic characterization of XMRV protease by a cell-free proteolytic analysis.2012
Author(s)
Matsunaga S, Sawasaki T, Ode H, Morishita R, Furukawa A, Sakuma R, Sugiura W, Sato H, Katahira M, Takaori-Kondo A, Yamamoto N, Ryo A
-
Journal Title
J Proteomics.
Volume: 75
Pages: 4863-4873
Peer Reviewed
-
-
-
-
-
[Journal Article] Specific In Situ Visualization of Plasma Cells Producing Antibodies Against Porphyromonas Gingivalis in Gingival Radicular Cyst: Application of the Enzyme-Labeled Antigen Method.2011
Author(s)
Tsuge S, Mizutani Y, Matsuoka K, Sawasaki T, Endo Y, Naruishi K, Maeda H, Takashiba S, Shiogama K, Inada KI, Tsutsumi Y.
-
Journal Title
J Histochem Cytochem
Volume: 59
Pages: 673-689
Peer Reviewed
-
-
-
-
-
-
-
[Journal Article] Evaluating the role of rheumatoid factors for the development of rheumatoid arthritis in a mouse model with a newly established ELISA system.2010
Author(s)
Tanaka Y, Komori H, Mori S, Soga Y, Tsubaki T, Terada M, Miyazaki T, Fujino T, Nakamura S, Kanno H, Sawasaki T, Endo Y, Nose M
-
Journal Title
Tohoku J Exp Med.
Volume: 220
Pages: 199-206
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
