2013 Fiscal Year Final Research Report
Development and innovation of chemical genomics platforms based on small molecule immobilization
| Project/Area Number |
22310132
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Living organism molecular science
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| Research Institution | Tohoku University |
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Principal Investigator |
KANOH NAOKI 東北大学, 薬学研究科(研究院), 准教授 (40317293)
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| Project Period (FY) |
2010-04-01 – 2014-03-31
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| Keywords | ケミカルバイオロジー / 低分子固定化担体 / ケミカルゲノミクス / P450 / 細胞内標的タンパク質 |
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| Research Abstract |
By utilizing small-molecule immobilization methods and thus obtained small molecule-immobilized solid supports, we have developed the following technologies: i.e., (1) a technology to identify cellular binding protein(s) for bioactive small molecules, and (2) a technology platform to identify substrate(s) for P450 enzymes of interest. Among them, the latter was realized by developing a fluorescent turn-on probe that react with NAD(P)+ produced during P450 substrate oxidation.
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